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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR1100 IGR1097 IGR1099 IGR1098 IGR1103 IGR1101 IGR1096 IGR1104 IGR1102 SMu1272 irvA, - SMu1274 SMu1269 SMu1268 SMu1270 pckA, - SMu1266 gbpC, - SMu1273 lepA, - SMu1271 SMu1267 SMu1275 SMu1272 irvA, - SMu1274 SMu1269 SMu1268 SMu1270 pckA, - SMu1266 gbpC, - SMu1273 lepA, - SMu1271 SMu1267 SMu1275 SMu1272 irvA, - SMu1274 SMu1268 SMu1270 pckA, - SMu1266 gbpC, - SMu1273 lepA, - SMu1271 SMu1269 SMu1267 SMu1275


LANL Gene ID: SMu1271

GenBank Locus Tag: SMU.1394c

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
lepA  

Definition:
GTP-binding protein LepA

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1323552

Gene Stop:
1321717

Gene Length:
1836

Molecular Weight*:
68349

pI*:
5.00

Net Charge*:
-16.94

EC:
 

Functional Class:
Cellular processes; Cell division  

Gene Ontology:
Biological process
  GO:0006413    translational initiation

Cellular component
  GO:0005622    intracellular

Molecular function
  GO:0003743    translation initiation factor activity
  GO:0003924    GTPase activity
  GO:0005525    GTP binding


Pathway: pathway table

Comment:
For other 'lep' genes see SMu0862 (lepA) and SMu1704 (lepC).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Several matches in gapped BLAST to GTP-binding proteins:residues 12-175 are 34% similar to the previously published tetracycline resistance protein in S.mutans (gi135623).

Residues 1-610 are 90% similar to GTP-binding protein in S.pyogenes (gi15675045) and 89% similar to gi15901063 from S.pneumoniae (gi15901063).







The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG0906 (0.0).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379799|ref|NP_721754.1|  GTP-binding protein LepA [Stre...  1180   0.0  
 gi|22537069|ref|NP_687920.1|  GTP-binding protein LepA [Stre...  1110   0.0  
 gi|77409570|ref|ZP_00786251.1|  GTP-binding protein LepA [St...  1109   0.0  
 gi|55820950|ref|YP_139392.1|  GTP-binding protein LepA [Stre...  1102   0.0  
 gi|55822871|ref|YP_141312.1|  GTP-binding protein LepA [Stre...  1102   0.0  
 gi|94992347|ref|YP_600446.1|  GTP-binding protein lepA [Stre...  1096   0.0  
 gi|15675045|ref|NP_269219.1|  GTP-binding protein LepA [Stre...  1095   0.0  
 gi|71903418|ref|YP_280221.1|  GTP-binding protein LepA [Stre...  1095   0.0  
 gi|157076361|gb|ABV11044.1|  GTP-binding protein LepA [Strep...  1093   0.0  
 gi|21910273|ref|NP_664541.1|  GTP-binding protein LepA [Stre...  1092   0.0


InterPro Summary:  InterProScan

InterPro
IPR000178
Family
Initiation factor 2
PD186100 [168-232]T 0.0 PD186100 IF2 IF2
InterPro
IPR000640
Domain
Translation elongation factor EFG/EF2, C-terminal
G3DSA:3.30.70.240 [410-500]T 5.39999384454116E-19 G3DSA:3.30.70.240 Transl_elong_EFG/EF2_C Transl_elong_EFG/EF2_C
PF00679 [407-496]T 2.5000090991620002E-36 PF00679 EFG_C EFG_C
InterPro
IPR000795
Domain
Protein synthesis factor, GTP-binding
PR00315 [15-28]T 2.1E-15 PR00315 ELONGATNFCT[58-66]T 2.1E-15 PR00315 ELONGATNFCT[83-93]T 2.1E-15 PR00315 ELONGATNFCT[99-110]T 2.1E-15 PR00315 ELONGATNFCT[135-144]T 2.1E-15 PR00315 ELONGATNFCT ELONGATNFCT
PF00009 [11-193]T 3.8999986179521805E-68 PF00009 GTP_EFTU GTP_EFTU
PS00301 [51-66]T 8.0E-5 PS00301 EFACTOR_GTP EFACTOR_GTP
InterPro
IPR004161
Domain
Translation elongation factor EFTu/EF1A, domain 2
PF03144 [214-284]T 1.19999985703101E-4 PF03144 GTP_EFTU_D2 GTP_EFTU_D2
InterPro
IPR005225
Domain
Small GTP-binding protein domain
TIGR00231 [11-187]T 2.9997133313710203E-15 TIGR00231 small_GTP small_GTP
InterPro
IPR006297
Family
GTP-binding protein LepA
TIGR01393 [11-605]T 0.0 TIGR01393 lepA lepA
InterPro
IPR009000
Domain
Translation elongation and initiation factors/Ribosomal, beta-barrel
SSF50447 [192-291]T 1.6E-23 SSF50447 Translat_factor Translat_factor
InterPro
IPR009022
Domain
Elongation factor G, III and V
SSF54980 [299-379]T 2.2E-22 SSF54980 EFG_III_V[410-533]T 8.8E-31 SSF54980 EFG_III_V EFG_III_V
InterPro
IPR013842
Domain
GTP-binding protein LepA, C-terminal
PF06421 [497-605]T 2.0000139277203602E-79 PF06421 LepA_C LepA_C
noIPR
unintegrated
unintegrated
G3DSA:3.30.70.870 [298-379]T 1.49999977583351E-5 G3DSA:3.30.70.870 G3DSA:3.30.70.870 G3DSA:3.30.70.870
G3DSA:3.40.50.300 [8-216]T 8.20001307135003E-60 G3DSA:3.40.50.300 G3DSA:3.40.50.300 G3DSA:3.40.50.300
PTHR23115 [9-317]T 0.0 PTHR23115 PTHR23115 PTHR23115
PTHR23115:SF40 [9-317]T 0.0 PTHR23115:SF40 PTHR23115:SF40 PTHR23115:SF40
SSF52540 [8-215]T 5.2E-60 SSF52540 SSF52540 SSF52540


COGS Summary:  COGS Search
BeTs to 13 clades of COG0481
COG name: Membrane GTPase LepA
Functional Class: N
The phylogenetic pattern of COG0481 is ----yqvcebrhujgpolinx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
***** IPB000640 (Elongation factor G, C-terminus) with a combined E-value of 2.1e-49.
    IPB000640A    13-38
    IPB000640B    50-72
    IPB000640C    82-109
    IPB000640D    116-144
    IPB000640E    181-192
    IPB000640F    404-438
***** IPB000795 (GTP-binding elongation factor) with a combined E-value of 9.7e-29.
    IPB000795A    15-30
    IPB000795B    85-116
    IPB000795C    120-144
***** IPB000178 (Initiation factor 2) with a combined E-value of 2.6e-16.
    IPB000178A    12-50
    IPB000178B    82-114
    IPB000178C    115-154


 ProDom Summary:  Protein Domain Search
Residues 427-486 are 81% similar to a (GTP-BINDING ELONGATION FACTOR BIOSYNTHESIS PROTEOME) protein domain (PD011419 which is seen in LEPA_LACLA.

Residues 188-298 are 81% similar to a (GTP-BINDING PROTEOME COMPLETE FACTOR) protein domain (PD004660 which is seen in LEPA_LACLA.

Residues 488-602 are 90% similar to a (GTP-BINDING PROTEOME COMPLETE MEMBRANE) protein domain (PD004661 which is seen in LEPA_LACLA.

Residues 25-183 are 34% similar to a (GTP-BINDING ADP-RIBOSYLATION MULTIGENE FAMILY ALPHA) protein domain (PD076602 which is seen in IF2_THEMA.

Residues 435-480 are 47% similar to a (GTP-BINDING III ZK1236.1 CHROMOSOME) protein domain (PD383064 which is seen in YO81_CAEEL.

Residues 16-143 are 36% similar to a (C215.12 GTP-BINDING CHROMOSOME II) protein domain (PD196535 which is seen in O94316_SCHPO.

Residues 99-185 are 96% similar to a (GTP-BINDING FACTOR INITIATION COMPLETE PROTEOME) protein domain (PD200055 which is seen in LEPA_LACLA.

Residues 303-425 are 74% similar to a (GTP-BINDING PROTEOME COMPLETE MEMBRANE) protein domain (PD117013 which is seen in LEPA_LACLA.

Residues 99-160 are 35% similar to a (FACTOR RELEASE GTP-BINDING CHAIN) protein domain (PD013442 which is seen in Q9RU41_DEIRA.

Residues 56-93 are 97% similar to a (FACTOR GTP-BINDING BIOSYNTHESIS INITIATION ELONGATION) protein domain (PD336744 which is seen in LEPA_LACLA.

Residues 427-486 are 48% similar to a (GTP-BINDING GUF1 MEMBRANE COMPLETE) protein domain (PD379297 which is seen in LEPA_ECOLI.

Residues 11-54 are 97% similar to a (GTP-BINDING FACTOR ELONGATION BIOSYNTHESIS RESISTANCE) protein domain (PD000122 which is seen in LEPA_LACLA.


Paralogs:  Local Blast Search
SMu1271 is paralogously related (blast p-value < 1e-3) to SMu0496, a predicted GTP-binding protein; SMu0554,a predicted peptide chain release factor 3;translation elongation and release factor; SMu0324,a predicted translation elongation factor G; SMu0382,a predicted translation initiation factor and SMu0651, a predicted translation elongation factor EF-Tu.


Pfam Summary:  Pfam Search
Residues 11 to 193 (E-value = 4.7e-71) place SMu1271 in the GTP_EFTU family which is described as Elongation factor Tu GTP binding domain (PF00009)
Residues 214 to 284 (E-value = 1.3e-06) place SMu1271 in the GTP_EFTU_D2 family which is described as Elongation factor Tu domain 2 (PF03144)
Residues 407 to 496 (E-value = 4.3e-40) place SMu1271 in the EFG_C family which is described as Elongation factor G C-terminus (PF00679)
Residues 497 to 605 (E-value = 1.7e-82) place SMu1271 in the LepA_C family which is described as GTP-binding protein LepA C-terminus (PF06421)

 Top PDB Hits:
pdb1FNMA Chain A, Structure Of Thermus Thermophilus Ef-G H573a 90 9e-019
pdb1ELO Elongation Factor G Without Nucleotide >gi1633250... 90 9e-019
pdb2EFGA Chain A, Translational Elongation Factor G Complexed... 90 9e-019

Gene Protein Sequence:
MTIEQLKNRQEKIRNFSIIAHIDHGKSTLADRILEQTETVSSREMQAQLL
DSMDLERERGITIKLNAIELNYKAKNGETYIFHLIDTPGHVDFTYEVSRS
LAACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPE
RVRTEIEDVIGLDASEAVLASAKAGIGIEEILEQIVEKVPAPKGDVEAPL
KALIFDSVYDAYRGVILQIRVMDGMVKPGDKIELMSNGKTFDVTEVGIFT
PKAVNRDFLATGDVGYLAASIKTVADTRVGDTVTLADCPAEAPLHGYKQL
NPMVFAGIYPIDSSKYNDMREALEKLQLNDASLHFEPETSQALGFGFRCG
FLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVYTTDGERLEVSNPSEFP
DPTKVDSIEEPYVKAQIMVPQEYVGAVMELSQRKRGDFVTMDYIDNNRVN
VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDISEYRKSKLVKMDILLNGD
KVDALSFIVHKEFAYERGKLIVEKLKKIIPRQQFEVPIQAAIGQKIVARS
DIKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLS
VLSMDDDDKKK

Gene Nucleotide Sequence:  Sequence Viewer
ATGACTATAGAACAACTTAAAAATCGTCAGGAGAAAATTCGTAATTTCTC
TATTATTGCCCATATAGACCATGGGAAGTCAACGCTGGCAGATCGTATTT
TGGAACAGACAGAAACTGTTTCTAGCAGAGAAATGCAAGCTCAGCTTTTG
GACAGCATGGACTTAGAACGTGAGCGTGGTATTACCATTAAATTAAATGC
GATTGAGCTTAATTATAAGGCTAAAAATGGTGAAACTTATATTTTTCACT
TGATTGACACGCCAGGACATGTTGACTTTACTTATGAAGTGTCACGTTCT
TTGGCAGCCTGTGAAGGTGCCATTTTGGTGGTAGATGCTGCACAAGGTAT
TGAAGCGCAGACCTTAGCCAATGTTTATTTGGCTTTAGATAATGATCTGG
AAATTTTACCTGTTATCAATAAAATTGACTTGCCAGCAGCTGATCCTGAA
CGTGTGCGCACTGAGATTGAAGATGTCATTGGACTGGATGCTAGTGAAGC
TGTTTTGGCTTCCGCCAAAGCAGGCATCGGGATTGAAGAGATTTTGGAGC
AAATTGTGGAAAAAGTACCAGCTCCAAAGGGGGATGTTGAAGCCCCACTC
AAAGCTCTTATTTTTGATTCTGTCTATGATGCTTATCGCGGCGTTATTCT
GCAAATTCGGGTCATGGATGGTATGGTCAAACCCGGAGATAAAATTGAAC
TCATGAGCAATGGCAAGACTTTTGACGTGACAGAAGTAGGAATTTTCACT
CCTAAGGCTGTTAATCGTGACTTTTTGGCAACGGGTGATGTAGGTTATTT
AGCTGCTTCAATCAAGACAGTGGCAGACACTCGTGTTGGTGATACAGTGA
CTCTGGCGGACTGCCCTGCCGAAGCACCTTTGCATGGTTATAAGCAGTTG
AATCCCATGGTTTTTGCAGGAATTTATCCTATTGATTCCAGCAAATATAA
TGACATGCGTGAAGCCCTAGAGAAATTACAGCTTAATGATGCCAGTCTTC
ACTTTGAACCTGAGACTTCTCAAGCCCTTGGCTTTGGTTTTCGTTGTGGC
TTCTTAGGTCTTTTGCATATGGATGTCATTCAAGAGCGATTGGAGCGTGA
GTTTAATATTGATCTCATTATGACAGCGCCATCTGTAGTTTACCATGTTT
ATACCACAGATGGGGAGAGGCTTGAGGTTTCTAATCCATCTGAATTTCCT
GATCCAACTAAGGTTGATAGTATTGAGGAACCTTACGTTAAAGCCCAGAT
TATGGTGCCGCAAGAGTATGTGGGAGCTGTCATGGAATTATCACAACGTA
AGCGCGGCGACTTTGTGACTATGGATTACATTGATAATAACCGAGTCAAT
GTTATTTACCAGATCCCTTTGGCTGAAATTGTTTTTGATTTTTTTGATAA
ACTAAAATCCTCTACCCGTGGTTATGCTAGTTTTGATTATGATATTTCTG
AGTATCGCAAGTCTAAGCTGGTAAAAATGGATATTTTGCTCAATGGTGAT
AAAGTTGATGCGCTTAGCTTCATTGTCCATAAAGAGTTTGCCTATGAAAG
GGGGAAATTAATTGTTGAAAAGTTGAAGAAGATCATTCCGCGGCAACAAT
TCGAAGTTCCCATTCAAGCAGCGATCGGTCAAAAAATTGTGGCGCGTTCT
GATATTAAGGCCTTGCGGAAAAATGTTCTTGCTAAATGTTATGGCGGAGA
TGTTTCCAGAAAGCGTAAACTTTTGGAAAAACAAAAGGCTGGTAAAAAGC
GGATGAAATCAATTGGTTCAGTTGAGGTCCCTCAGGAAGCCTTTCTCAGC
GTTCTGTCCATGGATGATGACGATAAGAAAAAATAG

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