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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR922 IGR919 IGR921 IGR925 IGR917 IGR918 IGR920 IGR923 IGR924 SMu1070 psaA, - SMu1068 ccdA, - SMu1071 SMu1073 SMu1072 cysD, - SMu1074 pcrA, - SMu1075 psaR,yjaD, - SMu1069 dagA, - SMu1076 SMu1070 psaA, - SMu1068 ccdA, - SMu1071 SMu1073 SMu1072 cysD, - SMu1074 pcrA, - SMu1075 psaR,yjaD, - SMu1069 dagA, - SMu1076 SMu1070 psaA, - SMu1068 ccdA, - SMu1071 SMu1073 SMu1072 cysD, - SMu1074 pcrA, - SMu1075 psaR,yjaD, - SMu1069 dagA, - SMu1076


LANL Gene ID: SMu1074

GenBank Locus Tag: SMU.1173c

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
cysD  

Definition:
O-acetylhomoserine sulfhydrylase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1113230

Gene Stop:
1111917

Gene Length:
1314

Molecular Weight*:
47448

pI*:
5.20

Net Charge*:
-12.17

EC:
4.2.99.10  

Functional Class:
Amino acid biosynthesis; Serine family  

Gene Ontology:
Biological process
  GO:0006355    regulation of transcription, DNA-dependent
  GO:0006520    amino acid metabolic process

Molecular function
  GO:0003700    transcription factor activity
  GO:0003824    catalytic activity
  GO:0016765    transferase activity, transferring alkyl or aryl (other than methyl) groups
  GO:0030170    pyridoxal phosphate binding


Pathway: pathway table
Cysteine metabolism
Methionine metabolism

Comment:
For other 'cys' genes see SMu0141 (cysS) and SMu0449 (cysK).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Several matches in gapped BLAST to O-acetylhomoserine sulfhydrylase : residues 60-437 are 74% similar to the enzyme in S.pneumoniae (15903138). Residues 11-436 are 59% similar to the protein from Clostridium acetobutylicum (15896038).





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG1098 (7e-04).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379603|ref|NP_721558.1|  putative O-acetylhomoserine su...   851   0.0  
 gi|157075321|gb|ABV10004.1|  O-acetylhomoserine (thiol)-lyas...   671   0.0  
 gi|125717698|ref|YP_001034831.1|  O-acetylhomoserine sulfhyd...   671   0.0  
 gi|116627784|ref|YP_820403.1|  O-acetylhomoserine sulfhydryl...   653   0.0  
 gi|55821020|ref|YP_139462.1|  O-acetylhomoserine sulfhydryla...   652   0.0  
 gi|146318687|ref|YP_001198399.1|  O-acetylhomoserine sulfhyd...   622   e-176
 gi|125974346|ref|YP_001038256.1|  O-acetylhomoserine/O-acety...   576   e-162
 gi|109648582|ref|ZP_01372481.1|  O-acetylhomoserine/O-acetyl...   544   e-153
 gi|89896938|ref|YP_520425.1|  hypothetical protein DSY4192 [...   543   e-153
 gi|148993824|ref|ZP_01823226.1|  O-acetylhomoserine aminocar...   541   e-152


InterPro Summary:  InterProScan

InterPro
IPR000277
Family
Cys/Met metabolism pyridoxal-phosphate-dependent enzymes
PTHR11808 [106-238]T 0.0 PTHR11808 Cys_Met_Meta_PP[273-437]T 0.0 PTHR11808 Cys_Met_Meta_PP Cys_Met_Meta_PP
PF01053 [19-435]T 0.0 PF01053 Cys_Met_Meta_PP Cys_Met_Meta_PP
PS00868 [211-225]T 8.0E-5 PS00868 CYS_MET_METAB_PP CYS_MET_METAB_PP
InterPro
IPR002197
Domain
Helix-turn-helix, Fis-type
PR01590 [102-119]T 2.4 PR01590 HTHFIS[119-139]T 2.4 PR01590 HTHFIS HTHFIS
InterPro
IPR006235
Family
O-acetylhomoserine/O-acetylserine sulfhydrylase
PTHR11808:SF12 [106-238]T 0.0 PTHR11808:SF12 OAH_OAS_sulfhy[273-437]T 0.0 PTHR11808:SF12 OAH_OAS_sulfhy OAH_OAS_sulfhy
TIGR01326 [17-435]T 0.0 TIGR01326 OAH_OAS_sulfhy OAH_OAS_sulfhy
InterPro
IPR015421
Domain
Pyridoxal phosphate-dependent transferase, major region, subdomain 1
G3DSA:3.40.640.10 [9-301]T 3.899998617952181E-91 G3DSA:3.40.640.10 PyrdxlP-dep_Trfase_major_sub1 PyrdxlP-dep_Trfase_major_sub1
InterPro
IPR015422
Domain
Pyridoxal phosphate-dependent transferase, major region, subdomain 2
G3DSA:3.90.1150.10 [302-435]T 3.2999907750333804E-39 G3DSA:3.90.1150.10 PyrdxlP-dep_Trfase_major_sub2 PyrdxlP-dep_Trfase_major_sub2
InterPro
IPR015424
Domain
Pyridoxal phosphate-dependent transferase, major region
SSF53383 [7-437]T 0.0 SSF53383 PyrdxlP-dep_Trfase_major PyrdxlP-dep_Trfase_major
noIPR
unintegrated
unintegrated
PIRSF001434 [20-437]T 0.0 PIRSF001434 CGS CGS


COGS Summary:  COGS Search
BeTs to 7 clades of COG0626
COG name: Cystathionine beta-lyases/cystathionine gamma-synthases
Functional Class: E
The phylogenetic pattern of COG0626 is ---kY-VcEBRHuj-------
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
***** IPB000277 (Cys/Met metabolism pyridoxal-phosphate-dependent enzymes) with a combined E-value of 2.3e-83.
    IPB000277A    64-85
    IPB000277B    157-190
    IPB000277C    194-229
    IPB000277D    283-323
    IPB000277E    380-396
    IPB000277F    413-430


 ProDom Summary:  Protein Domain Search
Residues 19-435 are 57% similar to a (AMINOTRANSFERASE PHOSPHATE PYRIDOXAL COMPLETE PROTEOME) protein domain (PD000087 which is seen in Q9CJC4_LACLA.


Paralogs:  Local Blast Search
SMu1074 is paralogously related (blast p-value < 1e-3) to SMu1527, a predicted cystathionine gamma-synthase and SMu0226, a predicted NifS protein homolog, class-V aminotransferase.


Pfam Summary:  Pfam Search
Residues 19 to 435 (E-value = 1e-198) place SMu1074 in the Cys_Met_Meta_PP family which is described as Cys/Met metabolism PLP-dependent enzyme (PF01053)
Residues 49 to 393 (E-value = 8.4e-06) place SMu1074 in the DegT_DnrJ_EryC1 family which is described as DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
92  
108

Top PDB Hits:
pdb1QGNA Chain A, Cystathionine Gamma-Synthase From Nicotiana... 201 1e-052
pdb1CS1A Chain A, Cystathionine Gamma-Synthase (Cgs) From Esc... 188 1e-048
pdb1CL2A Chain A, Cystathionine Beta-Lyase (Cbl) From Escheri... 131 2e-031

Gene Protein Sequence:
MELINNKRRASMTREFSFETLQLHAGQSVDPTTKSRAVPIYQTTSYVFND
AQDAEDSFALRTPGNIYTRITNPTTAVFEERMAALEGGVGALATASGMAA
VTYIALALAHAGDHIVSAATVYGGTFNLLKETLPRYGITTSFVDVANFAE
IEAAITDKTKFIIAETLGNPLGNIADLEKLAEIAHRHAIPLVIDNTFGTP
YLLNVFSYGVDIAVHSATKFIGGHGTSIGGVIVDSGNFDWEKSGKFPQFV
EPDPSYHDISYTRDIGKAAFVTAVRTQLLRDTGACLSPFNAFLLLQGLET
LSLRVERHVENAKKIAYYLENHPKVTKVNYASLPSSPYYDLAQKYLPKGA
SSIFTFNVAGSAKAAREVIDSLEIFSDLANVADAKSLVVHPATTTHGQMT
EEDLRACGIEPEQIRVSIGLENADDLIEDLRLALEKI

Gene Nucleotide Sequence:  Sequence Viewer
ATGGAGCTAATTAATAATAAAAGGAGAGCTTCCATGACTCGAGAATTTTC
TTTTGAAACTTTACAATTACATGCGGGACAAAGTGTTGATCCTACAACAA
AATCGCGTGCAGTACCAATCTATCAGACGACTTCCTATGTGTTTAATGAT
GCACAAGATGCTGAAGATTCTTTTGCACTTCGTACACCCGGCAATATTTA
TACGCGGATCACTAATCCGACTACAGCCGTTTTTGAAGAACGGATGGCCG
CTCTTGAAGGTGGTGTCGGTGCACTGGCAACAGCTTCTGGTATGGCAGCA
GTAACTTATATTGCCTTGGCTCTTGCTCATGCAGGTGATCATATTGTGTC
AGCAGCGACAGTTTACGGTGGCACTTTTAATCTTCTTAAGGAAACTTTAC
CTCGCTATGGCATTACTACAAGTTTTGTTGATGTTGCTAATTTCGCTGAA
ATTGAAGCGGCTATTACAGACAAGACTAAGTTTATTATCGCTGAAACGTT
AGGAAATCCTCTTGGAAATATCGCTGATCTTGAAAAATTAGCTGAGATTG
CCCATCGACATGCTATTCCCTTGGTTATTGATAATACCTTTGGTACTCCT
TATTTGCTTAATGTCTTCTCTTACGGTGTTGATATTGCTGTTCATTCTGC
CACTAAATTTATCGGTGGACATGGGACATCTATTGGCGGTGTCATTGTTG
ATTCTGGAAACTTTGATTGGGAAAAATCTGGAAAATTCCCACAATTTGTA
GAACCAGATCCTTCCTATCATGACATTAGTTATACACGTGATATTGGAAA
AGCAGCTTTTGTAACTGCGGTGCGTACGCAACTGCTGCGTGATACAGGCG
CCTGCCTTTCACCTTTCAATGCCTTTCTTTTGCTACAAGGTCTAGAAACC
TTATCACTTCGTGTTGAGCGTCATGTGGAAAATGCTAAGAAAATTGCGTA
CTATCTGGAAAATCATCCTAAAGTCACAAAAGTTAATTATGCTAGTTTGC
CATCAAGTCCTTATTATGACTTGGCTCAAAAATACTTGCCAAAAGGAGCT
AGTTCTATCTTTACTTTTAATGTTGCAGGCAGTGCGAAAGCCGCTCGCGA
GGTCATTGACAGTCTTGAAATCTTTTCTGATTTGGCGAATGTTGCTGATG
CCAAATCACTAGTTGTTCATCCGGCAACAACCACTCATGGTCAAATGACT
GAAGAAGATCTACGAGCTTGCGGTATTGAACCTGAGCAAATCCGTGTTTC
TATTGGTTTGGAAAATGCTGATGACTTAATCGAAGATTTGCGCCTAGCAC
TTGAAAAAATATAA

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