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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR650 IGR651 IGR645 IGR649 IGR647 IGR648 IGR653 IGR646 IGR652 mscL, - SMu0743 rpsU, - SMu0742 SMu0747 glnH, - SMu0741 rmlD, - SMu0748 rgpAc, - SMu0749 rpoD, - SMu0746 arcT, - SMu0740 SMu0744 dnaG, - SMu0745 mscL, - SMu0743 rpsU, - SMu0742 SMu0747 glnH, - SMu0741 rmlD, - SMu0748 rgpAc, - SMu0749 rpoD, - SMu0746 arcT, - SMu0740 SMu0744 dnaG, - SMu0745 mscL, - SMu0743 rpsU, - SMu0742 SMu0747 glnH, - SMu0741 rmlD, - SMu0748 rgpAc, - SMu0749 rpoD, - SMu0746 arcT, - SMu0740 SMu0744 dnaG, - SMu0745 rgpBc, - SMu0750 rgpBc, - SMu0750


LANL Gene ID: SMu0745

GenBank Locus Tag: SMU.821

DNA Molecule Name:
1  

GenBank ID:
24379276

Gene Name:
dnaG  

Definition:
DNA primase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
765772

Gene Stop:
767550

Gene Length:
1779

Molecular Weight*:
68101

pI*:
5.50

Net Charge*:
-13.50

EC:
2.7.7.-  

Functional Class:
DNA metabolism; DNA replication, recombination, and repair  

Gene Ontology:
Biological process
  GO:0006260    DNA replication
  GO:0006269    DNA replication, synthesis of RNA primer
  GO:0006304    DNA modification

Molecular function
  GO:0003676    nucleic acid binding
  GO:0003677    DNA binding
  GO:0003896    DNA primase activity
  GO:0008270    zinc ion binding


Pathway: pathway table
DNA polymerase
Ubiquinone biosynthesis

Secondary Evidence:
Araya-Kojima,T., Ishibashi,N., Shimamura,S., Tanaka,K. and
Takahashi,H.
Identification and molecular analysis of Lactococcus lactis rpoD
operon
Biosci. Biotechnol. Biochem. 59 (1), 73-77 (1995)
PubMed: 7765979

Versalovic,J. and Lupski,J.R.
The Haemophilus influenzae dnaG sequence and conserved bacterial
primase motifs
Gene 136 (1-2), 281-286 (1993)
PubMed: 8294018

Versalovic,J., Koeuth,T., Britton,R., Geszvain,K. and Lupski,J.R.
Conservation and evolution of the rpsU-dnaG-rpoD macromolecular
synthesis operon in bacteria
Mol. Microbiol. 8 (2), 343-355 (1993)
PubMed: 8316085



Comment:
For other 'dna' genes, see SMu0001 (dnaA); SMu0002 (dnaN); SMu0074
(dnaK);SMu075 (dnaJ); SMu1091 (dnaE); SMu1745 (dnaI); SMu1746
(dnaB);SMu1437 (dnaX),(dnaH) and SMu1940 (dnaC).

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [gi:1709769].





View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Several matches in gapped BLAST to DNA primase: residues 13-591 are 61% similar to the protein in S.pyogenes (15674826|). Residues 15-591 are 51% similar to the protein from S.pneumoniae
(15900941|).






The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG1427 (0.0).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379276|ref|NP_721231.1|  DNA primase [Streptococcus mut...  1123   0.0  
 gi|77406974|ref|ZP_00783991.1|  DNA primase [Streptococcus a...   753   0.0  
 gi|76787887|ref|YP_330070.1|  DNA primase [Streptococcus aga...   751   0.0  
 gi|77412515|ref|ZP_00788813.1|  DNA primase [Streptococcus a...   749   0.0  
 gi|116628188|ref|YP_820807.1|  DNA primase (bacterial type) ...   749   0.0  
 gi|55821466|ref|YP_139908.1|  DNA primase [Streptococcus the...   749   0.0  
 gi|22537573|ref|NP_688424.1|  DNA primase [Streptococcus aga...   748   0.0  
 gi|55823394|ref|YP_141835.1|  DNA primase [Streptococcus the...   748   0.0  
 gi|77409565|ref|ZP_00786247.1|  DNA primase [Streptococcus a...   744   0.0  
 gi|94994082|ref|YP_602180.1|  DNA primase [Streptococcus pyo...   735   0.0


InterPro Summary:  InterProScan

InterPro
IPR002694
Domain
Zinc finger, CHC2-type
PD002988 [13-86]T 6e-37 PD002988 Q8DUS6_STRMU_Q8DUS6; Q8DUS6_STRMU_Q8DUS6;
PF01807 [2-99]T 2.7e-48 PF01807 zf-CHC2 zf-CHC2
SM00400 [33-87]T 6.7e-31 SM00400 ZnF_CHCC ZnF_CHCC
InterPro
IPR006154
Domain
Toprim subdomain
SM00493 [260-331]T 6.8e-17 SM00493 TOPRIM TOPRIM
InterPro
IPR006171
Domain
TOPRIM
PF01751 [260-337]T 6e-18 PF01751 Toprim Toprim
InterPro
IPR006295
Domain
DNA primase
TIGR01391 [1-415]T 7.8e-143 TIGR01391 dnaG: DNA primase dnaG: DNA primase
InterPro
IPR006647
Domain
Toprim, primase
PD002276 [120-346]T 2e-127 PD002276 Q8DUS6_STRMU_Q8DUS6; Q8DUS6_STRMU_Q8DUS6;
InterPro
IPR010916
Domain
TonB box, N-terminal
PS00430 [1-88]? NA PS00430 TONB_DEPENDENT_REC_1 TONB_DEPENDENT_REC_1
InterPro
IPR013173
Domain
DNA primase DnaG, DnaB-binding
SM00766 [464-584]T 2.2e+02 SM00766 no description no description
InterPro
IPR013237
Domain
Phage P4 alpha, zinc-binding
SM00778 [32-68]T 23 SM00778 no description no description
InterPro
IPR013264
Domain
DNA primase catalytic core, N-terminal
G3DSA:3.90.980.10 [111-241]T 8.7e-33 G3DSA:3.90.980.10 no description no description
PF08275 [123-253]T 5.4e-50 PF08275 Toprim_N Toprim_N
noIPR
unintegrated
unintegrated
G3DSA:3.40.1360.10 [242-363]T 7.9e-37 G3DSA:3.40.1360.10 no description no description
G3DSA:3.90.580.10 [2-100]T 5.9e-35 G3DSA:3.90.580.10 no description no description


COGS Summary:  COGS Search
BeTs to 15 clades of COG0358
COG name: DNA primase (bacterial type)
Functional Class: L
The phylogenetic pattern of COG0358 is amtk-qvcebrhujGPolinx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
***** IPB002694 (CHC2 zinc finger) with a combined E-value of 1.4e-100.
    IPB002694A    37-82
    IPB002694B    139-159
    IPB002694C    193-217
    IPB002694D    229-248
    IPB002694E    263-300
    IPB002694F    302-315
    IPB002694G    336-346


 ProDom Summary:  Protein Domain Search
Residues 212-345 are 61% similar to a (DNA PRIMASE 2.7.7.- TRANSFERASE) protein domain (PD002276 which is seen in Q9CI14_LACLA.

Residues 93-172 are 38% similar to a (DNA PRIMASE REPLICATION METAL-BINDING) protein domain (PD003861 which is seen in Q9KD44_BACHD.

Residues 109-209 are 28% similar to a (DNA TRANSFERASE POLYMERASE PRIMASE) protein domain (PD290405 which is seen in PRIM_CLOAB.

Residues 367-590 are 25% similar to a (DNA PRIMASE 2.7.7.- TRANSFERASE) protein domain (PD119307 which is seen in Q9CI14_LACLA.

Residues 94-161 are 47% similar to a (DNA 2.7.7.- PRIMASE METAL-BINDING) protein domain (PD203954 which is seen in Q9CI14_LACLA.

Residues 33-88 are 57% similar to a (DNA PRIMASE 2.7.7.- TRANSFERASE) protein domain (PD002988 which is seen in Q9CI14_LACLA.

Residues 163-210 are 47% similar to a (DNA PRIMASE 2.7.7.- TRANSFERASE) protein domain (PD410978 which is seen in Q9CI14_LACLA.


Paralogs:  Local Blast Search
SMu0745 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.


Pfam Summary:  Pfam Search
Residues 2 to 99 (E-value = 9.5e-49) place SMu0745 in the zf-CHC2 family which is described as CHC2 zinc finger (PF01807)
Residues 260 to 337 (E-value = 8.4e-18) place SMu0745 in the Toprim family which is described as Toprim domain (PF01751)

 Top PDB Hits:
pdb|1DD9|A Chain A, Structure Of The Dnag Catalytic Core >gi|77... 152 1e-037
pdb|1EQN|A Chain A, E.Coli Primase Catalytic Core >gi|9256899|p... 144 2e-035
pdb|1D0Q|A Chain A, Structure Of The Zinc-Binding Domain Of Bac... 106 6e-024

Gene Protein Sequence:
MVVDKEKIKEIKDSINIVDVIGEVVSLSRSGRNYLGLCPFHKEKTPSFNV
IEDRQFFHCFGCGRSGDVFKFVEDYRNISFLESVQVLAERSGINVTLQDK
SQEVKKEHPHQKLFNINGDANKFYQAVLKTTKVGETARQYLYERGLTDEL
ITYFKIGLAPNEYDYLYQAMLKKYDEDTIINSGLFNLSDSNSIYDAFRNR
IMFPLTDEFGHIIGFSGRIWTEEDTSKKLAKYKNTRSTIIFNKSYELYHL
DKARTAAKKEHELYLMEGFMDVIAAYRAGIPNAVASMGTALTPEHVKHLR
KFTKKVILTYDGDKAGQNAIAKSLNLLSNMAVEIVRIPGQMDPDEFIKEN
SEEELEKLLKQARISSAEFWIEHLKPENIDNLQAEIDYVEKIAKVIAASP
SITAQNTYINKVAEFLPDFDYLQVEQAVNNERLQMRSQISEQESANQQVL
IDLPVTKQLTALTKAESQLFHRLLHHDYLLNEFRNRAHFKFTTPELQELY
QILKTNGEINAYDLSQLSNQLQETYYRVLEENLPEEIAEDEIRQIEDQID
KCLQRQEMRKQSKAIRESSNHGDVDTAVEGLAALIAQKRNME

Gene Nucleotide Sequence:  Sequence Viewer
CTGGTTGTTGATAAAGAGAAGATTAAAGAAATCAAAGACAGTATCAACAT
TGTTGATGTTATTGGTGAAGTTGTTAGTCTTTCACGCTCTGGTCGTAATT
ATTTAGGACTTTGTCCTTTTCACAAAGAAAAGACCCCTTCTTTTAATGTT
ATTGAAGATCGTCAATTTTTTCATTGCTTTGGTTGCGGCCGCTCAGGAGA
TGTTTTTAAATTTGTTGAGGATTATCGTAACATTAGCTTTCTGGAAAGCG
TTCAGGTTTTAGCTGAGCGTTCAGGTATTAATGTTACTCTTCAAGATAAG
AGTCAAGAAGTTAAAAAGGAACATCCACATCAAAAACTTTTTAATATCAA
TGGAGATGCGAACAAATTCTATCAAGCTGTTTTGAAGACAACGAAAGTTG
GAGAAACTGCACGGCAGTATCTTTATGAGAGAGGTTTGACAGATGAACTT
ATCACTTATTTTAAGATAGGTTTGGCTCCCAATGAGTATGATTATCTGTA
TCAGGCCATGCTCAAAAAATATGATGAAGATACAATTATTAATTCAGGTT
TGTTTAATCTATCAGATAGCAATTCCATTTATGATGCTTTTCGTAATCGG
ATCATGTTTCCTTTGACTGATGAATTTGGACACATTATAGGTTTTTCGGG
ACGGATTTGGACAGAAGAAGATACTTCTAAAAAATTAGCAAAGTATAAGA
ATACCCGCTCAACAATCATTTTTAATAAATCTTACGAACTTTACCATTTG
GATAAGGCTAGGACTGCAGCTAAAAAAGAACATGAACTTTATCTTATGGA
AGGCTTTATGGATGTTATTGCAGCCTATCGTGCGGGTATTCCTAATGCTG
TTGCTTCTATGGGGACAGCGCTAACACCAGAACATGTTAAGCATTTAAGA
AAATTTACTAAAAAAGTTATTTTGACCTATGATGGTGATAAAGCCGGACA
AAATGCTATTGCTAAATCATTGAACTTGCTGTCTAATATGGCGGTGGAAA
TTGTTAGAATTCCCGGACAGATGGATCCAGATGAATTTATCAAGGAGAAT
TCAGAAGAGGAATTAGAAAAGCTGCTAAAACAAGCAAGGATTAGCAGTGC
TGAGTTTTGGATTGAACATCTAAAACCTGAAAATATTGATAATTTGCAAG
CGGAAATTGACTATGTCGAAAAGATAGCTAAGGTTATTGCTGCTTCACCG
TCTATAACAGCTCAGAATACCTATATTAATAAAGTAGCTGAGTTTCTACC
AGATTTTGATTATTTACAGGTTGAACAGGCAGTTAATAATGAGCGACTGC
AAATGCGTTCACAAATCAGCGAACAAGAGTCAGCCAATCAGCAGGTTTTA
ATAGATTTGCCGGTAACTAAACAATTAACCGCTTTAACTAAAGCCGAGAG
TCAATTATTCCACCGCTTACTCCACCACGATTACCTCTTGAATGAATTTC
GTAATCGTGCTCATTTTAAATTTACAACGCCAGAATTACAAGAACTTTAT
CAAATTCTCAAAACTAACGGTGAGATTAATGCTTATGATTTGTCTCAACT
TTCCAATCAGCTTCAGGAAACTTATTATCGTGTTTTAGAAGAAAATCTTC
CTGAAGAAATTGCTGAAGATGAAATTAGGCAGATTGAAGATCAAATTGAT
AAGTGTTTGCAGCGACAAGAAATGCGCAAACAAAGCAAAGCTATTCGAGA
GAGTAGCAATCATGGTGATGTAGATACTGCAGTGGAGGGATTAGCAGCCC
TGATCGCTCAAAAAAGAAATATGGAATAG

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