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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap tRNA-Ser-5 IGR333 IGR335 IGR338 IGR339 IGR337 IGR334 IGR332 IGR340 IGR336 IGR341 SMu0373 SMu0381 rbfA, - SMu0383 SMu0378 trmB, - SMu0377 SMu0374 SMu0376 SMu0375 nusA, - SMu0379 infB, - SMu0382 SMu0373 SMu0381 rbfA, - SMu0383 SMu0378 trmB, - SMu0377 SMu0374 SMu0376 SMu0375 nusA, - SMu0379 infB, - SMu0382 Type: tandem, Name: SMu-TR-6 - 6 SMu0373 rbfA, - SMu0383 SMu0378 trmB, - SMu0377 SMu0374 SMu0376 SMu0375 nusA, - SMu0379 infB, - SMu0382 SMu0380 SMu0381 SMu0380


LANL Gene ID: SMu0379

GenBank Locus Tag: SMU.418

DNA Molecule Name:
1  

GenBank ID:
24378911

Gene Name:
nusA  

Definition:
transcription termination-antitermination factor NusA; elongation factor

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
390889

Gene Stop:
392082

Gene Length:
1194

Molecular Weight*:
45449

pI*:
4.60

Net Charge*:
-26.99

EC:
 

Functional Class:
Transcription; Transcription factors  

Gene Ontology:
Biological process
  GO:0031554    regulation of transcription termination

Molecular function
  GO:0003700    transcription factor activity
  GO:0003723    RNA binding
  GO:0005515    protein binding
  GO:0030528    transcription regulator activity


Pathway: pathway table

Secondary Evidence:
Gopal B, Haire LF, Gamblin SJ, Dodson EJ, Lane AN, Papavinasasundaram KG, Colston MJ, Dodson G.
Crystal Structure of the Transcription Elongation/Anti-termination Factor NusA from Mycobacterium tuberculosis at 1.7 A Resolution.
J Mol Biol. 2001 Dec 14;314(5):1087-95.
PMID: 11743725

Artsimovitch I, Svetlov V, Anthony L, Burgess RR, Landick R.
RNA polymerases from Bacillus subtilis and Escherichia coli differ in recognition of regulatory signals in vitro.
J Bacteriol. 2000 Nov;182(21):6027-35.
PMID: 11029421

Comment:
For other 'nus'genes see SMu1769 (nusG) and SMu1680 (nusB).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Matches in gapped BLAST to NusA proteins. Residues 1-384 are 81% similar to the predicted nusA from S.pyogenes (>gi15675574). Residues 1-378 are 78% similar to gi15902522 from S.pneumoniae.





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG0378 (1e-171).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24378911|ref|NP_720866.1|  transcription elongation facto...   685   0.0  
 gi|94989044|ref|YP_597145.1|  N utilization substance protei...   578   e-163
 gi|50914806|ref|YP_060778.1|  transcription elongation facto...   577   e-163
 gi|94991042|ref|YP_599142.1|  N utilization substance protei...   577   e-163
 gi|28895281|ref|NP_801631.1|  transcription elongation facto...   577   e-163
 gi|19746626|ref|NP_607762.1|  transcription elongation facto...   576   e-163
 gi|15675574|ref|NP_269748.1|  transcription elongation facto...   576   e-162
 gi|21911034|ref|NP_665302.1|  transcription elongation facto...   573   e-162
 gi|55820434|ref|YP_138876.1|  transcription elongation facto...   564   e-159
 gi|25010487|ref|NP_734882.1|  transcription elongation facto...   561   e-158


InterPro Summary:  InterProScan

InterPro
IPR003029
Domain
RNA binding S1
PF00575 [131-197]T 2.80000004689696 PF00575 S1 S1
SM00316 [133-199]T 0.044000000000000296 SM00316 S1 S1
InterPro
IPR004087
Domain
KH
SM00322 [229-306]T 1.0 SM00322 KH[315-393]T 0.025000000000000102 SM00322 KH KH
InterPro
IPR004088
Domain
KH, type 1
PF00013 [318-367]T 54.9999942632762 PF00013 KH_1 KH_1
PS50084 [316-357]T 0.0 PS50084 KH_TYPE_1 KH_TYPE_1
InterPro
IPR009019
Domain
KH, prokaryotic type
G3DSA:3.30.300.20 [213-305]T 1.2000011745814003E-22 G3DSA:3.30.300.20 KH_prok[306-358]T 8.60000664726984E-14 G3DSA:3.30.300.20 KH_prok KH_prok
SSF54814 [199-291]T 6.6E-23 SSF54814 KH_prok[292-358]T 7.4E-20 SSF54814 KH_prok KH_prok
InterPro
IPR010213
Domain
Transcription termination factor NusA
TIGR01953 [4-357]T 0.0 TIGR01953 NusA NusA
InterPro
IPR012340
Domain
Nucleic acid-binding, OB-fold
G3DSA:2.40.50.140 [150-212]T 5.799996538547801E-18 G3DSA:2.40.50.140 OB_NA_bd_sub OB_NA_bd_sub
InterPro
IPR013735
Domain
NusA N-terminal
PF08529 [4-125]T 4.3000017064587895E-57 PF08529 NusA_N NusA_N
noIPR
unintegrated
unintegrated
G3DSA:3.30.1480.10 [1-134]T 4.39999001237694E-40 G3DSA:3.30.1480.10 G3DSA:3.30.1480.10 G3DSA:3.30.1480.10
PTHR22648 [1-390]T 1.69998802370796E-103 PTHR22648 PTHR22648 PTHR22648
SSF50249 [131-198]T 1.4E-17 SSF50249 Nucleic_acid_OB Nucleic_acid_OB
SSF69705 [1-126]T 3.9E-38 SSF69705 SSF69705 SSF69705


COGS Summary:  COGS Search
BeTs to 16 clades of COG0195
COG name: Transcription terminator NusA
Functional Class: K
The phylogenetic pattern of COG0195 is amTK-qvcebrhujgpolinx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
No significant hits to the Blocks database.

 ProDom Summary:  Protein Domain Search
No significant hits to the ProDom database.

Paralogs:  Local Blast Search
SMu0379 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.


Pfam Summary:  Pfam Search
Residues 320 to 364 (E-value = 5e-05) place SMu0379 in the KH family which is described as KH domain (PF00013)

 Top PDB Hits:
No significant hits to the NCBI PDB database.

Gene Protein Sequence:
MSQEMLEAFRILEEEKHINKDDIIDAVKESLKSAYKRRYGQAESAEIEFD
EKSGDFKVFTVREVVEEVFDSRLEISLKDALAISTAYELGDKIRFEESPK
EFGRVAAQSAKQTIMEKMRRQMREVTFNEYKEHEREIMTGTVERFDNRFI
YVNLGTIEAQLSRQDQVPGETFASHDRIEVYVYKVENNPKGVNVFVSRSH
PEFIKRIMEQEIPEVFDGTVEIMSVAREAGDRTKVAVRSHNPNVDAIGTI
VGRKGANIKKVTSKFHPKRFDQKTGLWVPMEENVDVIEWVDDPAEFIYNA
VAPAEVDQVLFDEEDDKHATVVVPDNKLSLAIGRRGQNVRLAAHLTGYRI
DIKSASEFEEMEAEWAAQDETQEATYEEAPAEEVSELSQEFAAEDAK

Gene Nucleotide Sequence:  Sequence Viewer
ATGAGCCAAGAAATGCTAGAAGCCTTCCGTATTCTGGAAGAGGAAAAACA
CATTAACAAAGATGATATTATTGATGCGGTTAAAGAATCATTAAAGTCAG
CTTATAAACGTCGCTATGGGCAGGCAGAAAGTGCTGAGATTGAATTTGAC
GAAAAGTCAGGAGACTTTAAAGTCTTCACTGTTCGTGAAGTTGTTGAAGA
AGTTTTTGACAGCCGCCTTGAGATCAGCTTGAAGGACGCCCTTGCGATCA
GTACAGCCTATGAACTTGGAGATAAAATTCGTTTTGAAGAGTCTCCCAAG
GAATTTGGTCGCGTGGCTGCCCAGTCAGCTAAACAAACGATCATGGAAAA
AATGCGCCGTCAAATGCGTGAAGTCACTTTTAATGAGTATAAAGAGCATG
AAAGAGAAATCATGACAGGGACTGTTGAGCGTTTTGATAATCGCTTTATC
TATGTTAATTTAGGAACGATAGAAGCGCAGCTGTCACGTCAAGATCAAGT
TCCGGGTGAAACGTTTGCTTCTCATGATCGCATTGAAGTTTATGTTTATA
AAGTAGAAAACAATCCTAAGGGCGTCAATGTCTTCGTTAGTCGCAGTCAT
CCTGAATTTATCAAGCGTATTATGGAACAGGAAATTCCTGAAGTTTTTGA
TGGCACTGTTGAAATTATGAGCGTAGCACGTGAAGCTGGTGATCGAACGA
AGGTTGCGGTTCGCAGTCATAATCCTAATGTTGATGCTATTGGAACTATA
GTTGGCCGCAAAGGTGCCAACATTAAGAAAGTGACCAGTAAATTCCATCC
TAAACGTTTTGATCAAAAAACTGGACTTTGGGTTCCAATGGAAGAGAATG
TTGATGTTATTGAGTGGGTTGATGATCCAGCAGAATTCATTTATAATGCT
GTTGCTCCTGCTGAGGTTGATCAAGTTCTCTTTGATGAAGAAGATGACAA
ACATGCAACAGTTGTTGTACCAGACAATAAGCTGTCACTTGCTATTGGCC
GTCGTGGTCAAAATGTCCGTTTAGCCGCTCATTTGACTGGCTATCGTATT
GATATTAAGTCTGCTAGTGAATTTGAAGAAATGGAAGCCGAGTGGGCAGC
ACAAGATGAAACCCAAGAGGCTACTTATGAAGAGGCTCCTGCTGAGGAAG
TTTCTGAACTCTCTCAAGAATTTGCAGCAGAAGATGCAAAATAG

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