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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR298 IGR299 IGR301 SMu0334 SMu0333 SMu0332 SMu0335 gltD, - SMu0331 gltA,gltB, - SMu0330 SMu0334 SMu0333 SMu0332 SMu0335 gltD, - SMu0331 gltA,gltB, - SMu0330 SMu0334 SMu0333 SMu0332 SMu0335 gltD, - SMu0331 gltA,gltB, - SMu0330 SMu0336 SMu0336


LANL Gene ID: SMu0331

GenBank Locus Tag: SMU.366

DNA Molecule Name:
1  

GenBank ID:
24378863

Gene Name:
gltD  

Definition:
glutamate synthase, small subunit

Cellular Location:
Cytoplasm, Extracellular [Evidence]

Gene Start:
347579

Gene Stop:
349015

Gene Length:
1437

Molecular Weight*:
53070

pI*:
5.10

Net Charge*:
-11.17

EC:
1.4.1.13  

Functional Class:
Amino acid biosynthesis; Glutamate family  

Gene Ontology:
Biological process
  GO:0006118    electron transport
  GO:0006537    glutamate biosynthetic process
  GO:0008152    metabolic process

Cellular component
  GO:0016020    membrane

Molecular function
  GO:0016491    oxidoreductase activity
  GO:0016639    oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor
  GO:0050660    FAD binding
  GO:0051536    iron-sulfur cluster binding


Pathway: pathway table
Glutamate metabolism
Nitrogen metabolism

Secondary Evidence:
Bohannon,D.E. and Sonenshein,A.L.
Positive regulation of glutamate biosynthesis in Bacillus subtilis
J. Bacteriol. 171 (9), 4718-4727 (1989)
PubMed: 2548995

Goss TJ, Perez-Matos A, Bender RA.
Roles of glutamate synthase, gltBD, and gltF in nitrogen metabolism of Escherichia coli and Klebsiella aerogenes.
J Bacteriol. 2001 Nov;183(22):6607-19.
PMID: 11673431

Dincturk HB.
Glutamate synthase: an archaeal horizontal gene transfer?
J Biosci. 2001 Mar;26(1):13-4. No abstract available.
PMID: 11255509


Comment:
For the large subunit, see SMu0330.

For other 'glt' genes see SMu0741 (gltS); SMu0296 (gltX) and SMu0330 (gltA),(gltB).





View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Matches in gapped BLAST to glutamate synthase small subunits. Residues 1-477 are 67% similar to gi15673267 from L.lactis. Residues 1-473 are 50% similar to gi5305491 from C.acetobutylicum. See also the similarity to SMu0762.





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG1834 (4e-06).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24378863|ref|NP_720818.1|  NADPH-dependent glutamate synt...   987   0.0  
 gi|15673267|ref|NP_267441.1|  glutamate synthase small subun...   683   0.0  
 gi|116512128|ref|YP_809344.1|  NADPH-dependent glutamate syn...   680   0.0  
 gi|125624014|ref|YP_001032497.1|  glutamate synthase, small ...   678   0.0  
 gi|116495986|ref|YP_807720.1|  NADPH-dependent glutamate syn...   636   0.0  
 gi|116617929|ref|YP_818300.1|  NADPH-dependent glutamate syn...   534   e-150
 gi|153813411|ref|ZP_01966079.1|  hypothetical protein RUMOBE...   489   e-136
 gi|18476144|gb|AAK62675.1|  putative glutamate synthase [Ent...   484   e-135
 gi|154503129|ref|ZP_02040189.1|  hypothetical protein RUMGNA...   476   e-132
 gi|119476526|ref|ZP_01616877.1|  glutamate synthase, NADH/NA...   473   e-131


InterPro Summary:  InterProScan

InterPro
IPR000759
Family
Adrenodoxin reductase
PR00419 [157-179]T 3.6E-24 PR00419 ADXRDTASE[180-193]T 3.6E-24 PR00419 ADXRDTASE[222-232]T 3.6E-24 PR00419 ADXRDTASE[297-311]T 3.6E-24 PR00419 ADXRDTASE ADXRDTASE
InterPro
IPR001100
Family
Pyridine nucleotide-disulphide oxidoreductase, class I
PR00411 [157-179]T 1.2E-9 PR00411 PNDRDTASEI[296-321]T 1.2E-9 PR00411 PNDRDTASEI[441-448]T 1.2E-9 PR00411 PNDRDTASEI PNDRDTASEI
InterPro
IPR001327
Domain
Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region
PD000139 [248-318]T 0.0 PD000139 FAD_pyr_redox FAD_pyr_redox
PF00070 [157-190]T 6.30000254573025E-5 PF00070 Pyr_redox Pyr_redox
InterPro
IPR003042
Family
Aromatic-ring hydroxylase
PR00420 [157-179]T 5.2E-7 PR00420 RNGMNOXGNASE[438-453]T 5.2E-7 PR00420 RNGMNOXGNASE RNGMNOXGNASE
InterPro
IPR006005
Domain
Glutamate synthase, NADH/NADPH, small subunit 1
TIGR01317 [3-475]T 0.0 TIGR01317 GOGAT_sm_gam GOGAT_sm_gam
InterPro
IPR009051
Domain
Alpha-helical ferredoxin
SSF46548 [4-166]T 8.3E-37 SSF46548 Helical_ferredxn Helical_ferredxn
InterPro
IPR012285
Domain
Fumarate reductase, C-terminal
G3DSA:1.10.1060.10 [33-142]T 8.70001261213413E-26 G3DSA:1.10.1060.10 Fum_reductase_C Fum_reductase_C
InterPro
IPR013027
Domain
FAD-dependent pyridine nucleotide-disulphide oxidoreductase
PR00368 [157-179]T 1.1E-11 PR00368 FADPNR[296-321]T 1.1E-11 PR00368 FADPNR[441-448]T 1.1E-11 PR00368 FADPNR FADPNR
PF07992 [157-452]T 3.49999466863949E-22 PF07992 Pyr_redox_2 Pyr_redox_2
noIPR
unintegrated
unintegrated
G3DSA:3.40.50.720 [152-315]T 9.00000407957216E-48 G3DSA:3.40.50.720 G3DSA:3.40.50.720 G3DSA:3.40.50.720
PTHR11938 [6-473]T 0.0 PTHR11938 PTHR11938 PTHR11938
PTHR11938:SF9 [6-473]T 0.0 PTHR11938:SF9 PTHR11938:SF9 PTHR11938:SF9


COGS Summary:  COGS Search
BeTs to 10 clades of COG0493
COG name: NADPH-dependent glutamate synthase beta chain and related oxidoreductases
Functional Class: E,R
The phylogenetic pattern of COG0493 is ---KYqVcEbR------l--x
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
***** PR00419 (Adrenodoxin reductase family signature) with a combined E-value of 2.9e-26.
    PR00419A    157-179
    PR00419B    180-193
    PR00419C    222-232
    PR00419D    297-311
    PR00419E    334-347
***** PR00368 (FAD-dependent pyridine nucleotide reductase signature) with a combined E-value of 2.5e-16.
    PR00368A    157-179
    PR00368B    244-253
    PR00368C    296-321
    PR00368E    441-448
***** IPB000171 (Bacterial-type phytoene dehydrogenase) with a combined E-value of 3.6e-12.
    IPB000171A    159-189
***** IPB000103 (Pyridine nucleotide-disulphide oxidoreductase class-II) with a combined E-value of 4e-08.
    IPB000103A    157-177
    IPB000103C    262-310
    IPB000103E    436-473
***** PR00420 (Aromatic-ring hydroxylase (flavoprotein monooxygenase) signature) with a combined E-value of 6.2e-07.
    PR00420A    157-179
    PR00420C    438-453


 ProDom Summary:  Protein Domain Search
No significant hits to the ProDom database.

Paralogs:  Local Blast Search
SMu0331 is paralogously related (blast p-value < 1e-3) to SMu0762, a predicted glutathione reductase, and to SMu0116, a predicted dihydrolipoamide dehydrogenase, to SMu0732, an ABC element, and to SMu0162, an oxidoreductase.


Pfam Summary:  Pfam Search
Residues 157 to 456 (E-value = 7e-21) place SMu0331 in the Pyr_redox family which is described as Pyridine nucleotide-disulphide oxidoreductase (PF00070)

 Top PDB Hits:
pdb1E1LA Chain A, Structure Of Adrenodoxin Reductase In Compl... 68 3e-012

Gene Protein Sequence:
MADPFGFLKYERKDNPYRPVNERIKDFEELQTTLSVEERQKQAARCMNCG
IPFCHEGTFYGGGRAVSGCPNDNLIPEWNDLIYKGDFRRAFERLTRTNPL
PEMTGRVCPAPCEKACTEGLNGSGVTIHDNERFIIDNAFEEGWVADSGRP
IERTGFKVAVVGSGPAGLSAAWRLNQLGHSVTVFERSDRFGGLLMYGIPN
MKLDKKIVQRRIDTMASIGINFVANTEIGRDITAEELLEKFDRVILATGA
SVPRDLDIPGRDLKGIRFAVDFLTETTKNLLDSDTHELPPLLEGKHVLVI
GGGDTGNDCIGTAVRLGAASVRQLEITPQLPEKRLPTNPWPQYPMINRTG
YGQEEADFVQQTDLTDYITSTVEFLGENGQVTAVKTIKVGPGFKAIEGTE
EVIKADLVLLAMGFTGAEKALFDQFRVECVYDDYSTRNEKVFVAGDARRG
PSLVIWGIREGRKTAEKIDQNLRMMVTE

Gene Nucleotide Sequence:  Sequence Viewer
ATGGCAGATCCGTTTGGATTTTTAAAGTATGAGCGCAAGGACAATCCCTA
TCGTCCTGTTAATGAGCGTATCAAAGATTTTGAAGAATTGCAGACAACTT
TATCAGTTGAGGAACGTCAAAAGCAAGCTGCTCGCTGTATGAATTGTGGG
ATCCCTTTCTGTCATGAGGGAACTTTCTATGGTGGCGGTCGTGCTGTTTC
AGGTTGTCCCAATGACAATTTAATCCCTGAATGGAATGATTTGATTTATA
AGGGCGATTTCAGACGTGCTTTTGAACGCTTGACACGAACTAACCCTTTA
CCAGAAATGACAGGTCGTGTCTGCCCGGCACCTTGTGAAAAGGCCTGTAC
AGAAGGCTTAAATGGTTCGGGTGTTACCATTCATGATAATGAACGTTTTA
TTATTGATAATGCATTTGAAGAAGGCTGGGTAGCTGATTCTGGTCGCCCT
ATTGAGCGTACAGGTTTTAAAGTAGCTGTCGTAGGTTCAGGTCCTGCTGG
CCTGTCAGCTGCTTGGCGTCTTAACCAGTTGGGTCACAGTGTAACTGTGT
TTGAGCGTTCTGATCGCTTTGGTGGTTTGCTCATGTATGGTATTCCCAAT
ATGAAATTGGATAAGAAAATTGTGCAACGCCGTATTGATACGATGGCTTC
AATTGGTATTAATTTTGTTGCCAATACAGAAATTGGTCGTGATATTACAG
CAGAAGAATTATTAGAAAAATTTGACCGTGTAATCTTGGCAACTGGAGCT
AGCGTTCCTCGTGACTTGGATATTCCAGGCCGTGACTTAAAAGGCATTCG
ATTTGCTGTTGATTTCTTAACAGAAACGACTAAAAATCTTTTGGACAGTG
ACACACACGAATTACCGCCCTTGCTTGAAGGAAAACATGTCTTAGTTATT
GGCGGTGGCGATACTGGAAATGACTGTATCGGTACGGCTGTTCGTTTGGG
AGCTGCTAGTGTTAGACAATTAGAAATTACGCCGCAACTGCCTGAAAAGC
GTCTGCCAACAAATCCATGGCCACAATATCCTATGATTAATCGTACAGGC
TATGGACAGGAAGAAGCCGATTTTGTTCAACAGACTGATTTGACGGACTA
TATCACGTCAACAGTAGAATTTCTTGGTGAAAATGGCCAAGTGACTGCGG
TGAAGACAATTAAGGTTGGACCTGGTTTCAAAGCCATTGAAGGAACTGAA
GAGGTAATTAAGGCAGATCTTGTTCTTCTTGCTATGGGCTTCACAGGTGC
TGAAAAAGCGCTCTTTGACCAATTCCGAGTTGAATGTGTTTATGATGACT
ATTCAACTCGAAATGAGAAGGTTTTTGTTGCAGGTGATGCACGCCGTGGT
CCTAGTCTTGTTATTTGGGGAATTCGAGAAGGACGCAAAACAGCAGAGAA
AATTGATCAAAACCTCAGAATGATGGTTACAGAGTGA

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