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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap tRNA-Gly-2 tRNA-Gly-1 tRNA-Val-1 tRNA-Thr-1 tRNA-Asp-1 tRNA-Phe-1 tRNA-Ile-1 tRNA-Met-1 tRNA-Met-2 tRNA-Met-3 tRNA-Pro-1 tRNA-Arg-1 tRNA-Lys-1 tRNA-Leu-1 tRNA-Leu-2 tRNA-Ser-2 tRNA-Ser-1 rRNA-5S-1 IGR17 IGR15 IGR21 IGR16 IGR18 IGR20 IGR13 acpP,ACP, - SMu0023 mreD,rodB, - SMu0017 comA, - SMu0024 purC, - SMu0025 mreC, - SMu0016 prsA,kprS, - SMu0019 plsX,ylpD, - SMu0022 aspC, - SMu0020 gbpB,pcsB,sagA, - SMu0018 acpP,ACP, - SMu0023 mreD,rodB, - SMu0017 comA, - SMu0024 purC, - SMu0025 mreC, - SMu0016 prsA,kprS, - SMu0019 plsX,ylpD, - SMu0022 aspC, - SMu0020 gbpB,pcsB,sagA, - SMu0018 Type: tandem, Name: SMu-TR-1 - 1 Type: tandem, Name: SMu-TR-1 - 1 acpP,ACP, - SMu0023 mreD,rodB, - SMu0017 purC, - SMu0025 mreC, - SMu0016 prsA,kprS, - SMu0019 plsX,ylpD, - SMu0022 aspC, - SMu0020 gbpB,pcsB,sagA, - SMu0018 recO, - SMu0021 recO, - SMu0021 comA, - SMu0024


LANL Gene ID: SMu0019

GenBank Locus Tag: SMU.23

DNA Molecule Name:
1  

GenBank ID:
24378551

Gene Name:
prsA  kprS  

Definition:
phosphoribosyl pyrophosphate synthetase (PRPP synthetase)

Cellular Location:
Cytoplasm, Membrane [Evidence]

Gene Start:
26502

Gene Stop:
27470

Gene Length:
969

Molecular Weight*:
35160

pI*:
5.90

Net Charge*:
-4.44

EC:
2.7.6.1  

Functional Class:
Energy metabolism; Pentose phosphate pathway  
Purines, pyrimidines, nucleosides, and nucleotides; Purine ribonucleotide biosynthesis  

Gene Ontology:
Biological process
  GO:0009116    nucleoside metabolic process
  GO:0009156    ribonucleoside monophosphate biosynthetic process
  GO:0009165    nucleotide biosynthetic process
  GO:0044249    cellular biosynthetic process

Molecular function
  GO:0000287    magnesium ion binding
  GO:0004749    ribose phosphate diphosphokinase activity
  GO:0016740    transferase activity
  GO:0016978    lipoate-protein ligase B activity


Pathway: pathway table
Pentose phosphate cycle
Purine metabolism

Secondary Evidence:
Drouault S, Anba J, Bonneau S, Bolotin A, Ehrlich SD, Renault P.
The peptidyl-prolyl isomerase motif is lacking in PmpA, the PrsA-like protein involved in the secretion machinery of Lactococcus lactis.
Appl Environ Microbiol. 2002 Aug;68(8):3932-42.
PMID: 12147493

Nilsson,D., Hove-Jensen,B. and Arnvig,K.
Primary structure of the tms and prs genes of Bacillus subtilis
Mol. Gen. Genet. 218 (3), 565-571 (1989)
PubMed: 2555671

Eriksen,T.A., Kadziola,A., Bentsen,A.K., Harlow,K.W. and Larsen,S.
Structural basis for the function of Bacillus subtilis
phosphoribosyl-pyrophosphate synthetase
Nat. Struct. Biol. 7 (4), 303-308 (2000)
PubMed: 10742175



Comment:
For other 'prs' genes see SMu0958 (prsA),(prs).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Several matches in gapped BLAST to PRPP sequences, for instance residues 1-317 are 91% similar to the enzyme in S.pyogenes (gi15674264) and 87% similar to the enzyme in S.pneumoniae (gi15902072).

See Spy0020.





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG0018 (1e-152).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24378551|ref|NP_720506.1|  ribose-phosphate pyrophosphoki...   590   e-167
 gi|50913364|ref|YP_059336.1|  ribose-phosphate pyrophosphoki...   537   e-151
 gi|15674264|ref|NP_268437.1|  ribose-phosphate pyrophosphoki...   536   e-151
 gi|94987649|ref|YP_595750.1|  ribose-phosphate pyrophosphoki...   536   e-151
 gi|56808894|ref|ZP_00366604.1|  COG0462: Phosphoribosylpyrop...   536   e-151
 gi|71902684|ref|YP_279487.1|  ribose-phosphate pyrophosphoki...   533   e-150
 gi|157074741|gb|ABV09424.1|  ribose-phosphate diphosphokinas...   527   e-148
 gi|55822016|ref|YP_140457.1|  ribose-phosphate pyrophosphoki...   523   e-147
 gi|116626993|ref|YP_819612.1|  Phosphoribosylpyrophosphate s...   523   e-147
 gi|55820126|ref|YP_138568.1|  ribose-phosphate pyrophosphoki...   523   e-147


InterPro Summary:  InterProScan

InterPro
IPR000836
Domain
Phosphoribosyltransferase
PF00156 [141-274]T 1.10000150671643E-31 PF00156 Pribosyltran Pribosyltran
InterPro
IPR000842
Family
Phosphoribosyl pyrophosphate synthetase
PS00114 [130-145]T 8.0E-5 PS00114 PRPP_SYNTHETASE PRPP_SYNTHETASE
InterPro
IPR002375
Family
Purine/pyrimidine phosphoribosyl transferase
PS00103 [220-232]F 0.0 PS00103 PUR_PYR_PR_TRANSFER PUR_PYR_PR_TRANSFER
InterPro
IPR005946
Family
Ribose-phosphate pyrophosphokinase
TIGR01251 [6-317]T 3.24208057410435E-125 TIGR01251 ribP_PPkin ribP_PPkin
noIPR
unintegrated
unintegrated
G3DSA:3.40.50.2020 [4-172]T 3.39999972437687E-67 G3DSA:3.40.50.2020 G3DSA:3.40.50.2020 G3DSA:3.40.50.2020
PTHR10210 [1-321]T 0.0 PTHR10210 PTHR10210 PTHR10210
PTHR10210:SF14 [1-321]T 0.0 PTHR10210:SF14 PTHR10210:SF14 PTHR10210:SF14
SSF53271 [71-310]T 5.8E-64 SSF53271 SSF53271 SSF53271


COGS Summary:  COGS Search
BeTs to 15 clades of COG0462
COG name: Phosphoribosylpyrophosphate synthetase
Functional Class: F,E
The phylogenetic pattern of COG0462 is AmtkYqvcebrhujgpol---
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
***** IPB000842 (Phosphoribosyl pyrophosphate synthetase) with a combined E-value of 5.6e-121.
    IPB000842A    36-78
    IPB000842B    106-151
    IPB000842C    166-193
    IPB000842D    196-250
    IPB000842E    253-280
    IPB000842F    293-316
***** IPB002375 (Purine/pyrimidine phosphoribosyl transferase) with a combined E-value of 5.8e-06.
    IPB002375B    217-232


 ProDom Summary:  Protein Domain Search
Residues 36-218 are 30% similar to a (PROTEOME PHOSPHORIBOSYL TRANSFERASE) protein domain (PD102473 which is seen in O51494_BORBU.

Residues 6-218 are 69% similar to a (TRANSFERASE PYROPHOSPHOKINASE RIBOSE-PHOSPHATE) protein domain (PD001439 which is seen in O33924_CORAM.

Residues 8-202 are 29% similar to a (TRANSFERASE PHOSPHORIBOSYL SYNTHASE) protein domain (PD306086 which is seen in Q9XGA1_SPIOL.

Residues 5-218 are 42% similar to a (TRANSFERASE PHOSPHORIBOSYLPYROPHOSPHATE) protein domain (PD035825 which is seen in Q9VT33_DROME.

Residues 280-317 are 63% similar to a (PROTEOME COMPLETE KINASE RIBOSE-PHOSPHATE) protein domain (PD378133 which is seen in Q9CHB8_LACLA.

Residues 221-303 are 27% similar to a (PROTEOME COMPLETE PYROPHOSPHOKINASE) protein domain (PD413639 which is seen in O29666_ARCFU.

Residues 221-279 are 44% similar to a (PYROPHOSPHOKINASE RIBOSE-PHOSPHATE) protein domain (PD375317 which is seen in KPRS_SALTY.

Residues 220-279 are 65% similar to a (TRANSFERASE SYNTHETASE PHOSPHORIBOSYL PYROPHOSPHOKINASE) protein domain (PD016748 which is seen in Q9CHB8_LACLA.


Paralogs:  Local Blast Search
This protein is highly similar to SMu0958, also a predicted PRPP. Residues 5-318 are 53% similar to SMu0958.

SMu0019 is paralogously related (blast p-value < 1e-3) to SMu0958.


Pfam Summary:  Pfam Search
Residues 141 to 274 (E-value = 9.9e-35) place SMu0019 in the Pribosyltran family which is described as Phosphoribosyl transferase domain (PF00156)

 Top PDB Hits:
pdb1DKRB Chain B, Crystal Structures Of Bacillus Subtilis Pho... 391 7e-110

Gene Protein Sequence:
MSYSNLKLFALSSNKELAEKVAKTIGISLGKSTVRQFSDGEIQVNIEESI
RGNHVFILQSTSSPVNDNLMEILIMVDALKRASAETVSVVMPYYGYARQD
RKARSREPITSKLVANMLAVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGA
PLIADYFVRRGMCGSDYVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRNV
NKMNTSEVMNIIGNVSGKTCILIDDMIDTAGTIAHAADALAEAGATAVYA
SCTHPVLSGSALDNIQNSAIEKLVVLDTIDLPEEKLIDKIEQISITDLIS
EAIIRIHEKRPLSPLFEFTTFN

Gene Nucleotide Sequence:  Sequence Viewer
ATGTCTTATTCTAATTTAAAATTGTTTGCTTTATCTTCAAACAAAGAATT
AGCAGAGAAAGTGGCTAAGACTATTGGTATTAGTCTTGGAAAATCAACTG
TTCGTCAATTTTCTGATGGAGAAATTCAAGTTAATATTGAAGAATCTATT
CGCGGAAACCATGTTTTTATTTTACAGTCAACAAGTTCACCTGTAAATGA
TAACTTAATGGAAATTTTGATTATGGTTGATGCTCTTAAACGTGCTAGTG
CTGAGACTGTCAGTGTTGTCATGCCTTATTATGGTTATGCTCGTCAAGAT
CGAAAAGCACGTTCGCGTGAACCCATCACCTCCAAGTTAGTTGCTAATAT
GCTTGCTGTGGCGGGAGTTGATCGTCTTTTGACTGTTGACTTGCATGCAG
CACAAATTCAAGGTTTTTTTGATATTCCTGTTGATCATTTGATGGGAGCC
CCTTTAATTGCGGATTATTTTGTTCGTCGTGGTATGTGTGGCAGCGACTA
TGTCGTTGTTAGTCCTGACCATGGAGGCGTGACACGTGCGCGTAAATTAG
CCCAATTTCTTCAAACTCCGATAGCAATTATTGATAAACGACGAAATGTT
AACAAAATGAATACAAGTGAAGTCATGAATATCATTGGCAATGTTTCTGG
GAAAACGTGTATTTTAATTGATGATATGATTGATACTGCAGGAACAATTG
CTCATGCAGCTGACGCCCTTGCTGAAGCAGGTGCTACTGCAGTTTATGCT
TCATGTACACATCCAGTTCTGTCTGGTTCTGCTTTAGATAATATTCAAAA
TTCGGCTATTGAAAAATTAGTTGTTTTGGATACAATTGACCTTCCTGAAG
AAAAATTGATAGATAAAATTGAACAAATTTCAATTACTGATTTGATTTCT
GAGGCTATTATTCGTATTCATGAAAAACGACCGCTATCACCTTTATTTGA
ATTTACAACTTTTAACTAG

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