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Letter
Bartonella henselae
and Domestic Cats, Jamaica
Locksley L. McV. Messam,* Rickie W. Kasten,* Megan J. Ritchie,† and
Bruno B. Chomel*
*University of California, Davis, California, USA; and †Jamaica Society
for Prevention of Cruelty to Animals, Kingston, Jamaica
Suggested
citation for this article
To the Editor: Bartonella henselae has been isolated from
domestic cats in most countries where it has been investigated (1),
with the exception of some countries at northern latitudes, such as Norway
(2). The prevalence of both bacteremia and seropositivity
in cats is usually highest in warm and humid tropical countries. The worldwide
distribution of cat scratch disease (CSD), a zoonotic disease caused mainly
by the scratch of a B. henselae–infected cat, follows a similar
pattern. Limited information is available about CSD in either humans or
the feline reservoir in the Caribbean region.
In 1955, 3 febrile children (siblings) admitted to a hospital in Havana,
Cuba, were diagnosed with CSD based on their symptoms and the positive
results of intradermal tests using the Foshay antigen (3).
The results of the bacteriologic examination, however, were negative.
All 3 siblings had previous contact with a female cat and her 4 kittens.
In 2003, Alvarez et al. (4) reported the case of a 13-year-old
Cuban boy who was treated for symptoms compatible with CSD. However, no
other information could be found in the scientific literature regarding
the isolation of this bacterium from domestic cats in the Caribbean or
seropositivity for B. henselae in humans or animals living in that
region.
In the summer of 2003, an employee at a veterinary clinic in Kingston,
Jamaica, was scratched and bitten on the hand by a cat. Fever and an enlarged
axillary lymph node developed in the employee, and CSD was suspected.
To confirm the clinical suspicion, and with the employee's permission,
a serum sample was taken 7 weeks after the incident. Whole blood from
the 62 remaining cats in the cattery was also collected into EDTA-containing
tubes and stored at 4°C before being shipped to California for testing.
The cat involved in the incident was not available for testing. The age
of 63% of the cats ranged from 1 month to >5 years. Forty percent of
the cats were formerly owned and put up for adoption and 16% of the cats
were strays. The cat's origin was not recorded for the remaining 44% of
the cats.
Upon reception at the laboratory, all cat blood samples were frozen at
–70°C. They were subsequently thawed, and aliquot plated onto 5% rabbit
blood–enriched agar and incubated at 37°C in 5% CO2 for
≤4 weeks. The EDTA tube supernatant was serogically tested for B.
henselae (mixed type I and type II antigens) by using a standard indirect
immunofluorescence assay (5). The 62 blood samples were
cultured and 12 (19.3%) cats were bacteremic for B. henselae. None
of the cultures yielded B. clarridgeiae or B. koehlerae.
Of the 12 bacteremic cats, 5 (42%) had positive cultures for B. henselae
type Houston I, and 7 (58%) had positive cultures for B. henselae
type Marseille, based on restriction fragment length polymorphism profile
of the 16S rDNA, by using DdeI enzyme (6). The
median number of CFUs was 385/mL (range 147–25,300). For the 5 cats infected
with B. henselae type Houston I, the median was 259 (range 147–513)
CFU/mL; for the 7 cats infected with B. henselae type Marseille,
the median was 534 (range 174–25,300) CFU/mL. Of the 5 cats that were
bacteremic for B. henselae Houston I, 2 were seronegative. Similarly,
2 of the 7 B. henselae type Marseille–bacteremic cats were seronegative.
These 4 seronegative cats, 4–10 weeks old, were most likely in the early
phase of bacteremia. None of the cats were co-infected with both subtypes.
When a titer of ≥1:64 was used, 37 (60%) cats were seropositive for
B. henselae. Their age ranged from a few weeks to >5 years old
(median 11 months), including 7 cats that were <6 months old. The employee's
B. henselae titer was 1:64.
These results constitute the first report originating from the Caribbean
region of B. henselae isolation from domestic cats, as well as
confirming seropositivity in a human, despite a low titer. Because we
were not able to obtain a blood sample from the suspect animal, we cannot
prove that this cat was the source of the employee's infection. Nevertheless,
this study confirms the existence of both B. henselae types I and
II in Jamaica, even if no specific conclusions can be drawn with regard
to their relative prevalence.
The Caribbean has the highest incidence of HIV/AIDS outside of sub-Saharan
Africa, with Jamaica having a HIV prevalence of 1.2% (range 0.6%–2.2%)
for persons 15–49 years of age (7). As B. henselae
is known to cause bacillary angiomatosis and bacillary peliosis in immunocompromised
persons, knowledge of its presence in the Jamaican cat population is important
for primary prevention. Unfortunately, diagnostic tests for B. henselae
are not currently available on the island.
Acknowledgments
We thank Lenworth
Jackson for facilitating the collection of the human serum sample.
Dr. Messam is a
veterinarian from Jamaica who is currently pursuing a PhD in epidemiology
at the University of California, Davis. His research interests are in
the epidemiology of zoonotic diseases and epidemiologic methods.
References
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CA, et al. Bartonella
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- Joint United Nations Program on HIV/AIDS. UNAIDS 2004 Report on the
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and data, end 2003. p. 15 [cited 25 Jan 2005]. Available from http://www.unaids.org/bangkok2004/GAR2004_pdf/GAR2004_table_countryestimates_en.pdf
Suggested citation
for this article:
Messam LL, Kasten RW,
Ritchie MJ, ChomelBB. Bartonella henselae and domestic cats, Jamaica
[letter]. Emerg Infect Dis [serial on the Internet]. 2005 Jul [date
cited]. Available from http://www.cdc.gov/ncidod/EID/vol11no07/05-0115.htm
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