Volume 11, Number 10, October 2005

Botulinum Neurotoxin Detection and Differentiation by Mass Spectrometry

John R. Barr,* Hercules Moura,* Anne E. Boyer,* Adrian R. Woolfitt,* Suzanne R. Kalb,* Antonis Pavlopoulos,* Lisa G. McWilliams,† Jurgen G. Schmidt,‡ Rodolfo A. Martinez,‡ and David L. Ashley*
*Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †Battelle Memorial Institute, Atlanta, Georgia, USA; and ‡Los Alamos National Laboratory, Los Alamos, New Mexico, USA

Appendix Figure. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) spectrum of botulinum neurotoxin (BoNT) reactions. The top panel shows the MALDI-TOF-MS spectrum of the mixture of substrate peptides with no toxin present. The second panel shows this same mixture of substrate peptides and BoNT-A. The third, fourth and fifth panels show the mixture of substrate peptides with BoNT-B, -E, and –F, respectively. The insert mass spectrum for BoNT-E distinguishes the BoNT-dependent N-terminal product from the BoNT-A substrate peptide.

EID Home | Top of Page | Ahead-of-Print | Past Issues | Suggested Citation | EID Search | Contact Us | Accessibility | Privacy Policy Notice | CDC Home | CDC Search | Health Topics A-Z

This page last reviewed August 24, 2005

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention