RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Windowpane ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Scophthalmus aquosus
FDA Seafood List Common Name: Windowpane
FDA Seafood List Market Name: Flounder
Unique IEF Gel Code: 19950831gel08
Unique Web Code: wph01Srr
Tissue Aliquot Code: scopaquo H94-003 #04m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	105	13.2	0.0243284	9	0.342426	16.6	9.8	4.55	107.1	9.2	0.0210876
2	5.2	199	25.1	0.0176463	6	0.181034	8.8	5.2	5.2	199.3	6.4	0.0158394
3	5.85	310	39	0.0384258	5	0.338888	16.5	9.7	5.85	309.8	5	0.0380551
4	6.55	414	52.1	0.023785	12	0.546404	26.6	15.6	6.55	409.8	11.8	0.026047
5	6.85	453	57.1	0.0092587	7	0.0873944	4.2	2.5	6.85	452.7	6.5	0.00754035
6	7.35	514	64.7	0.0103867	8	0.123871	6	3.5	7.35	515.8	8	0.00873572
7	8.15	638	80.4	0.0192009	7	0.24138	11.7	6.9	8.15	636.3	7.2	0.0187902
8	8.45	706	88.9	0.0210653	6	0.196483	9.5	5.6	8.45	704.8	6	0.0185333

PeakCounter: 8

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.945	21	2.7	0.0193791	9	0.283542	1.4	1.1	3.945	23.1	9.3	0.0171417
2	4.225	61	7.7	0.0152919	4	0.105059	0.5	0.4	4.225	59.5	4.3	0.0137926
3	4.316	74	9.3	0.200223	4	1.04862	5.1	3.9	4.316	74.3	3.9	0.151591
4	4.428	90	11.4	0.0956003	15	2.69429	13.1	10.1	4.428	86.2	14.9	0.101918
5	4.651	122	15.4	0.181382	5	1.53308	7.5	5.7	4.651	122.5	5.1	0.169482
6	5.374	230	29	0.354026	5	3.33534	16.2	12.5	5.374	230	5.3	0.358185
7	5.445	242	30.6	0.171541	4	1.23798	6	4.6	5.445	241.9	4	0.172943
8	5.628	273	34.5	0.260915	4	2.06881	10.1	7.7	5.628	272.4	4.5	0.260187
9	5.693	284	35.9	0.104637	6	1.14607	5.6	4.3	5.693	283.5	6.3	0.102855
10	5.788	300	37.9	0.0362733	5	0.299289	1.5	1.1	5.788	300.5	4.7	0.0356362
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.847	310	39.1	0.0348579	5	0.329352	1.6	1.2	5.847	310.9	5.3	0.0349105
12	5.965	328	41.4	0.0303601	4	0.198832	1	0.7	5.965	328.1	3.8	0.0298013
13	6.07	344	43.4	0.0566968	2	0.0902983	0.4	0.3	6.07	341.3	1.8	0.0288347
14	6.11	350	44.2	0.150093	6	1.54129	7.5	5.8	6.11	350.6	6	0.144476
15	6.182	361	45.6	0.0701987	5	0.55539	2.7	2.1	6.182	361.3	5	0.0632003
16	6.267	374	47.2	0.406842	3	2.25417	11	8.4	6.267	373.9	3.1	0.410355
17	6.405	395	49.9	0.183063	3	1.02095	5	3.8	6.405	394.7	3.4	0.17171
18	7.367	515	65	0.0648307	9	0.821612	4	3.1	7.367	515.6	8.7	0.0532205

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	110	13.9	0.0225794	6	0.217717	12.6	8.2	4.55	110.2	5.7	0.0216626
2	5.2	202	25.6	0.0144249	6	0.134614	7.8	5.1	5.2	202.1	5.8	0.0129839
3	5.85	311	39.4	0.0314953	5	0.268019	15.5	10.1	5.85	310.8	5	0.0300923
4	6.55	420	53.2	0.0277103	13	0.590795	34.2	22.2	6.55	414.5	13.1	0.0253519
5	7.35	511	64.7	0.00614306	7	0.0632976	3.7	2.4	7.35	510.5	6.9	0.00514138
6	8.15	623	78.9	0.0165972	7	0.208599	12.1	7.8	8.15	623.6	7.3	0.0161029
7	8.45	694	87.8	0.0195204	8	0.245703	14.2	9.2	8.45	693.3	8.5	0.0163708

PeakCounter: 7

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.885	25	3.2	0.0133088	7	0.154956	1.2	0.9	3.885	26	7.4	0.0118225
2	4.318	83	10.5	0.0821568	5	0.71781	5.4	4.2	4.318	82.6	5.2	0.0778063
3	4.423	97	12.3	0.0259701	10	0.496311	3.7	2.9	4.423	95.4	10.4	0.0268106
4	4.55	114	14.5	0.0160297	3	0.0856101	0.6	0.5	4.55	113.3	3.2	0.0150133
5	4.655	128	16.2	0.0895308	4	0.703051	5.3	4.1	4.655	128.8	4.4	0.0910736
6	4.864	156	19.8	0.00831567	6	0.077816	0.6	0.5	4.864	157	5.7	0.00767056
7	5.2	201	25.5	0.012383	7	0.135849	1	0.8	5.2	203.5	7.4	0.0103466
8	5.439	241	30.6	0.214877	7	2.60725	19.6	15.2	5.439	241.8	6.7	0.218443
9	5.517	254	32.2	0.0784408	4	0.44234	3.3	2.6	5.517	253.9	3.6	0.0692709
10	5.69	283	35.9	0.16591	5	1.42391	10.7	8.3	5.69	282.7	5	0.160033
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.762	295	37.4	0.0804392	6	0.875851	6.6	5.1	5.762	294.4	6.1	0.0811185
12	5.872	313	39.7	0.0816596	6	0.878084	6.6	5.1	5.872	313.3	6.4	0.077227
13	5.944	324	41.1	0.0292647	5	0.221112	1.7	1.3	5.944	323.5	4.6	0.0271802
14	6.056	341	43.3	0.0180437	3	0.0997953	0.7	0.6	6.056	340.5	3.3	0.0170381
15	6.195	362	45.9	0.112127	7	1.23466	9.3	7.2	6.195	361.8	7.4	0.0942054
16	6.32	381	48.4	0.25706	3	1.53252	11.5	8.9	6.32	380.8	3.4	0.25279
17	6.445	400	50.8	0.127759	3	0.549868	4.1	3.2	6.445	399.8	3.2	0.0975561
18	6.55	416	52.8	0.0182988	16	0.796487	6	4.6	6.55	406.7	15.9	0.0282505
19	7.35	512	65	0.0388534	4	0.281484	2.1	1.6	7.35	511.4	4.3	0.0373592

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.93	29	3.7	0.0197193	10	0.30357	1.9	1.4	3.93	30.5	9.7	0.0176668
2	4.227	69	8.8	0.0312447	7	0.374332	2.3	1.7	4.227	66.4	7.2	0.0295172
3	4.331	83	10.5	0.145895	4	0.869423	5.4	3.9	4.331	83.9	3.8	0.129009
4	4.45	99	12.6	0.0684576	11	1.3142	8.2	5.9	4.45	96.1	10.7	0.0694896
5	4.598	119	15.1	0.0197346	4	0.10799	0.7	0.5	4.598	118.1	3.6	0.016915
6	4.687	131	16.6	0.138471	3	0.807639	5	3.6	4.687	131.2	3.2	0.140716
7	5.446	241	30.6	0.376999	4	2.95313	18.5	13.2	5.446	240.3	4.4	0.37702
8	5.506	251	31.9	0.185009	3	0.952266	6	4.3	5.506	250.6	2.9	0.185698
9	5.56	260	33	0.0239204	5	0.196795	1.2	0.9	5.56	260.6	4.9	0.0225211
10	5.662	277	35.2	0.301628	3	1.80053	11.3	8.1	5.662	277.3	3.4	0.296821
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.728	288	36.6	0.114191	6	1.11412	7	5	5.728	287.4	5.6	0.11205
12	5.818	303	38.5	0.043567	3	0.213791	1.3	1	5.818	303.5	2.9	0.0415491
13	5.88	313	39.8	0.0356556	5	0.284242	1.8	1.3	5.88	314.1	4.7	0.0342777
14	5.997	331	42.1	0.0320927	3	0.182762	1.1	0.8	5.997	331.5	3.2	0.0320692
15	6.114	349	44.3	0.0690218	2	0.234768	1.5	1.1	6.114	347.4	2.3	0.0574927
16	6.147	354	45	0.136751	4	0.789425	4.9	3.5	6.147	354	3.9	0.115183
17	6.179	359	45.6	0.0672261	1	0.112524	0.7	0.5	6.179	358.9	1.4	0.0449027
18	6.296	377	47.9	0.397596	3	1.95147	12.2	8.7	6.296	376.5	2.7	0.413707
19	6.407	394	50.1	0.174683	3	0.915791	5.7	4.1	6.407	394.3	3.3	0.158716
20	7.317	505	64.2	0.0612111	6	0.516398	3.2	2.3	7.317	505.1	5.7	0.0514312

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	110	14	0.0348256	5	0.304208	13.3	8.8	4.55	109.9	5.1	0.0338092
2	5.2	198	25.2	0.020536	7	0.235957	10.3	6.8	5.2	196.7	7	0.0191133
3	5.85	306	39	0.0412062	5	0.316977	13.9	9.2	5.85	305.8	4.7	0.0380954
4	6.55	415	52.9	0.0299266	13	0.642229	28.2	18.6	6.55	410.5	12.9	0.0281497
5	6.85	447	56.9	0.0106233	6	0.0955603	4.2	2.8	6.85	448.8	5.8	0.0092791
6	7.35	505	64.3	0.0134791	6	0.140751	6.2	4.1	7.35	504.3	6.2	0.0128428
7	8.15	630	80.3	0.0213697	9	0.316474	13.9	9.1	8.15	629.2	9	0.0197538
8	8.45	702	89.4	0.0195026	8	0.228513	10	6.6	8.45	700.4	7.7	0.0166823

PeakCounter: 8

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 80

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 16.600 5.200 8.800 5.850 16.500 6.550 26.600 6.850 4.200 7.350 6.000 8.150 11.700 8.450 9.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 8 Return to Lane Navigator
3.945 1.400 4.225 0.500 4.316 5.100 4.428 13.100 4.651 7.500 5.374 16.200 5.445 6.000 5.628 10.100 5.693 5.600 5.788 1.500 5.847 1.600 5.965 1.000 6.070 0.400 6.110 7.500 6.182 2.700 6.267 11.000 6.405 5.000 7.367 4.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 18 Return to Lane Navigator
4.550 12.600 5.200 7.800 5.850 15.500 6.550 34.200 7.350 3.700 8.150 12.100 8.450 14.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 7 Return to Lane Navigator
3.885 1.200 4.318 5.400 4.423 3.700 4.550 0.600 4.655 5.300 4.864 0.600 5.200 1.000 5.439 19.600 5.517 3.300 5.690 10.700 5.762 6.600 5.872 6.600 5.944 1.700 6.056 0.700 6.195 9.300 6.320 11.500 6.445 4.100 6.550 6.000 7.350 2.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 19 Return to Lane Navigator
3.930 1.900 4.227 2.300 4.331 5.400 4.450 8.200 4.598 0.700 4.687 5.000 5.446 18.500 5.506 6.000 5.560 1.200 5.662 11.300 5.728 7.000 5.818 1.300 5.880 1.800 5.997 1.100 6.114 1.500 6.147 4.900 6.179 0.700 6.296 12.200 6.407 5.700 7.317 3.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator
4.550 13.300 5.200 10.300 5.850 13.900 6.550 28.200 6.850 4.200 7.350 6.200 8.150 13.900 8.450 10.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 8 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Windowpane ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Windowpane ---- Go to Display Gel Lane Navigator.