RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Widow Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes entomelas
FDA Seafood List Common Name: Widow Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19981215gel07
Unique Web Code: wdh01Srr
Tissue Aliquot Code: sebaento I94-001 #07p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	79	9.4	0.0870971	3	0.485875	4.9	3.6	4.55	79.5	3.2	0.0850122
2	5.2	171	20.4	0.102459	4	0.74688	7.6	5.6	5.2	170.8	4.3	0.098059
3	5.85	283	33.8	0.237075	3	1.33794	13.6	10	5.85	283.3	3.2	0.236042
4	6.55	426	50.8	0.145083	8	1.83795	18.7	13.7	6.55	424.2	7.5	0.137491
5	6.85	459	54.8	0.0554297	4	0.359512	3.7	2.7	6.85	459	3.9	0.0518936
6	7.35	527	62.9	0.114354	4	0.689662	7	5.2	7.35	527.1	3.5	0.110276
7	8.15	645	77	0.161936	6	1.52109	15.4	11.4	8.15	644.3	5.5	0.155831
8	8.45	706	84.2	0.1667	6	1.7256	17.5	12.9	8.45	705.4	6	0.161614
9	8.65	759	90.6	0.126569	4	0.861604	8.8	6.4	8.65	758.2	4	0.120301
10	9.3	812	96.9	0.0229864	8	0.279218	2.8	2.1	9.3	810.9	8	0.0197462

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.254	40	4.8	0.224661	12	4.23788	4.5	3.5	4.254	39.1	11.8	0.201973
2	4.709	103	12.3	1.4102	2	6.33885	6.8	5.2	4.709	103.2	2.4	1.46182
3	5.041	149	17.8	0.0965823	9	1.60192	1.7	1.3	5.041	148.1	9.3	0.0974796
4	5.186	169	20.2	0.0481989	2	0.16868	0.2	0.1	5.186	168.8	2.2	0.0427616
5	5.35	197	23.5	0.0149415	3	0.0775146	0.1	0.1	5.35	197.4	2.9	0.0150031
6	5.465	217	25.9	0.0309848	4	0.203799	0.2	0.2	5.465	217.3	4	0.0284342
7	5.626	245	29.2	0.0148132	4	0.100878	0.1	0.1	5.626	244	3.6	0.0156402
8	5.672	253	30.2	0.0236578	3	0.103684	0.1	0.1	5.672	253.3	2.6	0.0224677
9	5.804	276	32.9	0.0183467	2	0.0783468	0.1	0.1	5.804	276	2.3	0.0188561
10	5.855	285	34	0.0121524	4	0.0730639	0.1	0.1	5.855	284.9	3.7	0.0111009
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.904	295	35.2	0.0178859	2	0.0702013	0.1	0.1	5.904	294.5	2.3	0.0170198
12	5.944	303	36.2	0.0297882	4	0.212837	0.2	0.2	5.944	304.4	4.4	0.0270354
13	5.993	313	37.4	2.20752	3	12.7811	13.7	10.5	5.993	314.2	3.5	2.08673
14	6.077	330	39.4	2.2488	4	14.8997	16	12.2	6.077	331	4.1	2.07437
15	6.146	344	41.1	0.3555	6	4.0222	4.3	3.3	6.146	346.1	6.1	0.371326
16	6.17	349	41.6	1.82392	3	8.28429	8.9	6.8	6.17	350	3.1	1.49785
17	6.225	360	43	1.38103	3	5.79697	6.2	4.8	6.225	359.3	2.5	1.30582
18	6.294	374	44.6	0.0998337	3	0.527172	0.6	0.4	6.294	373	3.1	0.0962042
19	6.348	385	45.9	0.365639	6	3.39829	3.6	2.8	6.348	385.1	6.4	0.300302
20	6.461	408	48.7	0.200717	3	0.976499	1	0.8	6.461	408	2.8	0.19921
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.501	416	49.6	0.0967527	3	0.583361	0.6	0.5	6.501	416.8	3.1	0.105978
22	6.54	424	50.6	0.0449794	2	0.204031	0.2	0.2	6.54	423.6	2.5	0.0461095
23	6.668	439	52.4	0.0732961	5	0.560614	0.6	0.5	6.668	438.8	4.7	0.0677117
24	6.777	451	53.8	0.207043	4	1.26324	1.4	1	6.777	451.5	3.6	0.200269
25	7.181	503	60	0.0652606	13	1.37507	1.5	1.1	7.181	504.8	12.5	0.0620107
26	7.38	530	63.2	0.14257	2	0.464292	0.5	0.4	7.38	530.3	2	0.132434
27	7.521	551	65.8	0.276083	10	4.52871	4.9	3.7	7.521	549.9	10.3	0.249193
28	7.629	567	67.7	2.32295	2	9.87842	10.6	8.1	7.629	568.4	2.1	2.67869
29	8.474	712	85	0.0611651	15	1.13672	1.2	0.9	8.474	712.3	14.5	0.0442269
30	8.575	739	88.2	0.174065	18	5.04591	5.4	4.1	8.575	738.5	17.9	0.159209
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.713	764	91.2	0.135531	15	2.88635	3.1	2.4	8.713	764.1	14.5	0.11229
32	9.382	817	97.5	0.234311	4	1.38384	1.5	1.1	9.382	816.3	3.7	0.209663

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	79	9.5	0.0942215	4	0.652584	6.2	4.7	4.55	79.1	4	0.0923826
2	5.2	167	20	0.113145	5	0.973893	9.3	7	5.2	168.2	4.9	0.111341
3	5.85	281	33.7	0.240148	3	1.33334	12.8	9.5	5.85	281.1	3.2	0.235821
4	6.55	422	50.6	0.175093	7	1.98635	19	14.2	6.55	420.8	7	0.160056
5	6.85	455	54.6	0.0743512	4	0.535227	5.1	3.8	6.85	454.9	4.5	0.0674259
6	7.35	520	62.4	0.133103	3	0.801645	7.7	5.7	7.35	519.6	3.4	0.133291
7	8.15	640	76.7	0.145577	6	1.37751	13.2	9.9	8.15	640.2	5.6	0.139947
8	8.45	701	84.1	0.166425	6	1.72392	16.5	12.3	8.45	700.5	6.1	0.160697
9	8.65	755	90.5	0.13677	4	0.90414	8.7	6.5	8.65	754	4	0.128
10	9.3	805	96.5	0.0187005	6	0.153447	1.5	1.1	9.3	804.5	5.6	0.0155394

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.314	50	6	0.112713	11	2.10688	4.2	3.4	4.314	50.9	11.4	0.104161
2	4.55	83	10	0.0389013	15	0.724006	1.5	1.2	4.55	77.1	14.9	0.02748
3	4.764	113	13.6	0.10577	9	1.74464	3.5	2.8	4.764	109.9	8.8	0.111662
4	4.979	143	17.2	0.120838	15	3.22707	6.5	5.1	4.979	140.1	15.5	0.117549
5	5.2	174	20.9	0.0328525	3	0.122373	0.2	0.2	5.2	174.3	2.9	0.0237898
6	5.631	245	29.5	0.0146103	2	0.0501217	0.1	0.1	5.631	245.1	2.1	0.0134042
7	5.85	281	33.8	0.0803805	5	0.653523	1.3	1	5.85	280.1	5.1	0.0729284
8	5.983	307	36.9	2.37203	2	9.12165	18.3	14.5	5.983	306.7	2	2.54898
9	6.049	320	38.5	2.36503	2	11.1058	22.3	17.7	6.049	321.1	2.4	2.57414
10	6.151	340	40.9	0.434067	6	3.71285	7.5	5.9	6.151	339.1	5.7	0.370005
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.203	350	42.1	1.10667	3	5.07127	10.2	8.1	6.203	349.8	2.6	1.08654
12	6.279	365	43.9	0.0394223	3	0.177886	0.4	0.3	6.279	363.3	3	0.0329788
13	6.325	374	45	0.122219	7	1.37636	2.8	2.2	6.325	375.2	6.9	0.112934
14	6.453	399	48	0.0950148	3	0.562302	1.1	0.9	6.453	399.3	3.4	0.0944012
15	6.514	411	49.5	0.056304	5	0.478255	1	0.8	6.514	410.5	5.2	0.0520876
16	6.545	417	50.2	0.0567641	3	0.272791	0.5	0.4	6.545	418	3	0.0514035
17	6.686	433	52.1	0.022096	4	0.123076	0.2	0.2	6.686	432.3	3.6	0.0194974
18	6.814	447	53.8	0.106743	6	0.982767	2	1.6	6.814	447.9	5.7	0.0979659
19	7.21	499	60	0.031282	8	0.45325	0.9	0.7	7.21	498.7	8.2	0.0311215
20	7.386	523	62.9	0.0519226	5	0.392317	0.8	0.6	7.386	521.5	4.5	0.0489115
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.53	544	65.5	0.202052	7	2.31605	4.7	3.7	7.53	543.9	6.9	0.188688
22	7.645	561	67.5	0.810315	2	2.75395	5.5	4.4	7.645	560.4	1.9	0.827399
23	8.15	635	76.4	0.0309532	3	0.158211	0.3	0.3	8.15	635.8	3	0.029939
24	8.45	703	84.6	0.0522798	5	0.469333	0.9	0.7	8.45	702.7	5.3	0.0504195
25	8.544	727	87.5	0.0151383	5	0.13347	0.3	0.2	8.544	726.9	5	0.015008
26	8.65	754	90.7	0.0746686	8	0.83986	1.7	1.3	8.65	753.8	7.7	0.0613123
27	9.366	808	97.2	0.108392	4	0.652231	1.3	1	9.366	807.5	3.6	0.101102

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.266	41	4.9	0.191508	11	3.41589	4.7	3.7	4.266	40.6	11	0.175969
2	4.654	97	11.6	0.212573	5	1.69664	2.3	1.8	4.654	97.6	4.8	0.201019
3	4.986	145	17.4	0.0267165	6	0.243814	0.3	0.3	4.986	144.7	6.2	0.0220529
4	5.103	162	19.4	0.0391735	3	0.18157	0.3	0.2	5.103	162.7	2.5	0.0405405
5	5.41	210	25.2	0.0226043	3	0.121241	0.2	0.1	5.41	210.7	3.2	0.0216918
6	5.565	235	28.2	0.0116479	3	0.0571373	0.1	0.1	5.565	234.4	2.7	0.0119933
7	5.609	242	29.1	0.0179791	2	0.0669821	0.1	0.1	5.609	241.9	1.9	0.0193998
8	5.745	264	31.7	0.0285894	4	0.178539	0.2	0.2	5.745	263.5	3.7	0.0274012
9	5.788	271	32.5	0.0249879	4	0.174394	0.2	0.2	5.788	271.2	4	0.0249072
10	5.844	280	33.6	0.0169099	4	0.112879	0.2	0.1	5.844	280.5	3.8	0.016611
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.901	291	34.9	0.0269135	3	0.129735	0.2	0.1	5.901	291	2.8	0.0260392
12	5.957	302	36.3	2.17438	3	11.1273	15.4	12	5.957	302.1	3.1	2.04504
13	6.054	321	38.5	2.35293	3	13.3296	18.4	14.4	6.054	320.9	3.2	2.36466
14	6.126	335	40.2	0.1783	8	2.26986	3.1	2.4	6.126	337.7	7.6	0.16776
15	6.162	342	41.1	1.05717	2	3.62197	5	3.9	6.162	342	2.2	0.928909
16	6.208	351	42.1	2.28674	2	6.82772	9.4	7.4	6.208	351.2	1.7	2.33209
17	6.274	364	43.7	0.0918935	4	0.567862	0.8	0.6	6.274	363.7	3.5	0.0904153
18	6.32	373	44.8	0.239619	4	1.32773	1.8	1.4	6.32	373.1	3.8	0.197586
19	6.376	384	46.1	0.0618887	8	1.10107	1.5	1.2	6.376	379.6	7.7	0.0803154
20	6.443	397	47.7	0.142228	3	0.644534	0.9	0.7	6.443	397.1	2.7	0.133721
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.494	407	48.9	0.0582934	3	0.322805	0.4	0.3	6.494	407.3	3.1	0.0578457
22	6.535	415	49.8	0.0384026	3	0.207806	0.3	0.2	6.535	415.5	3	0.0388723
23	6.686	433	52	0.0728831	4	0.440579	0.6	0.5	6.686	432.2	4.1	0.061031
24	6.795	445	53.4	0.172536	4	1.07393	1.5	1.2	6.795	444.9	3.7	0.163611
25	7.172	495	59.4	0.0623531	13	1.39457	1.9	1.5	7.172	499.1	12.9	0.0609385
26	7.368	522	62.7	0.111578	2	0.379527	0.5	0.4	7.368	522.2	2.1	0.10082
27	7.509	543	65.2	0.302441	8	3.90334	5.4	4.2	7.509	543.6	8	0.275136
28	7.616	559	67.1	2.3362	2	9.00329	12.4	9.7	7.616	559.4	2	2.55652
29	8.452	705	84.6	0.0457274	18	1.10617	1.5	1.2	8.452	705.4	17.8	0.0349843
30	8.56	733	88	0.134781	16	3.0804	4.3	3.3	8.56	731	15.6	0.111467
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.644	755	90.6	0.122994	18	3.51154	4.9	3.8	8.644	756.5	17.6	0.112725
32	9.38	811	97.4	0.12863	4	0.769896	1.1	0.8	9.38	810.3	3.6	0.121403

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	79	9.5	0.111361	4	0.720767	6.3	4.7	4.55	78.9	3.7	0.110866
2	5.2	176	21.1	0.128359	5	1.16622	10.1	7.5	5.2	175.7	5.1	0.129768
3	5.85	279	33.4	0.250847	3	1.30694	11.4	8.4	5.85	278.6	3	0.246277
4	6.55	416	49.8	0.248833	5	2.16398	18.8	14	6.55	415.3	5.4	0.224058
5	6.85	449	53.8	0.0763435	5	0.541976	4.7	3.5	6.85	449.6	4.5	0.0675747
6	7.35	519	62.2	0.170276	3	0.995264	8.6	6.4	7.35	518.5	3.3	0.170054
7	8.15	640	76.6	0.14561	5	1.37343	11.9	8.9	8.15	640	5.5	0.141967
8	8.45	704	84.3	0.195485	6	1.9379	16.8	12.5	8.45	703.4	5.9	0.185743
9	8.65	757	90.7	0.155064	4	0.984223	8.6	6.4	8.65	757	3.7	0.150431
10	9.3	805	96.4	0.0287501	7	0.317514	2.8	2	9.3	802.8	7.5	0.0238976

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 121

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 4.900 5.200 7.600 5.850 13.600 6.550 18.700 6.850 3.700 7.350 7.000 8.150 15.400 8.450 17.500 8.650 8.800 9.300 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.254 4.500 4.709 6.800 5.041 1.700 5.186 0.200 5.350 0.100 5.465 0.200 5.626 0.100 5.672 0.100 5.804 0.100 5.855 0.100 5.904 0.100 5.944 0.200 5.993 13.700 6.077 16.000 6.146 4.300 6.170 8.900 6.225 6.200 6.294 0.600 6.348 3.600 6.461 1.000 6.501 0.600 6.540 0.200 6.668 0.600 6.777 1.400 7.181 1.500 7.380 0.500 7.521 4.900 7.629 10.600 8.474 1.200 8.575 5.400 8.713 3.100 9.382 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 32 Return to Lane Navigator
4.550 6.200 5.200 9.300 5.850 12.800 6.550 19.000 6.850 5.100 7.350 7.700 8.150 13.200 8.450 16.500 8.650 8.700 9.300 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.314 4.200 4.550 1.500 4.764 3.500 4.979 6.500 5.200 0.200 5.631 0.100 5.850 1.300 5.983 18.300 6.049 22.300 6.151 7.500 6.203 10.200 6.279 0.400 6.325 2.800 6.453 1.100 6.514 1.000 6.545 0.500 6.686 0.200 6.814 2.000 7.210 0.900 7.386 0.800 7.530 4.700 7.645 5.500 8.150 0.300 8.450 0.900 8.544 0.300 8.650 1.700 9.366 1.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 27 Return to Lane Navigator
4.266 4.700 4.654 2.300 4.986 0.300 5.103 0.300 5.410 0.200 5.565 0.100 5.609 0.100 5.745 0.200 5.788 0.200 5.844 0.200 5.901 0.200 5.957 15.400 6.054 18.400 6.126 3.100 6.162 5.000 6.208 9.400 6.274 0.800 6.320 1.800 6.376 1.500 6.443 0.900 6.494 0.400 6.535 0.300 6.686 0.600 6.795 1.500 7.172 1.900 7.368 0.500 7.509 5.400 7.616 12.400 8.452 1.500 8.560 4.300 8.644 4.900 9.380 1.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 32 Return to Lane Navigator
4.550 6.300 5.200 10.100 5.850 11.400 6.550 18.800 6.850 4.700 7.350 8.600 8.150 11.900 8.450 16.800 8.650 8.600 9.300 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Widow Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Widow Rockfish ---- Go to Display Gel Lane Navigator.