RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Skipjack Tuna ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Katsuwonus pelamis
FDA Seafood List Common Name: Skipjack Tuna
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970430gel01
Unique Web Code: skg02Srr
Tissue Aliquot Code: katspela E96-002 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	80	3.6	0.349755	25	14.5665	18.7	15	3.5	83.8	25.5	0.322794
2	4.55	397	17.7	0.365129	8	4.73236	6.1	4.9	4.55	396.9	8.1	0.331415
3	5.2	571	25.5	0.521495	8	7.09106	9.1	7.3	5.2	570.7	7.9	0.504719
4	5.85	878	39.2	0.793797	6	7.95856	10.2	8.2	5.85	878.8	5.5	0.81381
5	6.55	1210	54.1	0.493547	9	7.32826	9.4	7.6	6.55	1209.6	8.9	0.463658
6	6.85	1297	58	0.367262	10	6.25886	8.1	6.5	6.85	1296.7	10.1	0.34974
7	7.35	1474	65.9	0.480316	10	8.46891	10.9	8.7	7.35	1472.3	10.1	0.473822
8	8.15	1764	78.8	0.115491	17	3.565	4.6	3.7	8.15	1763.3	17.5	0.115191
9	8.45	1883	84.1	0.189603	12	3.82857	4.9	3.9	8.45	1882.7	11.8	0.182612
10	8.65	1956	87.4	0.194971	9	2.95296	3.8	3	8.65	1955.5	8.9	0.187679
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	8.991	2087	93.3	0.0976976	16	2.72601	3.5	2.8	8.991	2090.6	15.7	0.0981706
12	9.3	2206	98.6	0.706372	7	8.25026	10.6	8.5	9.3	2205.7	6.8	0.680297

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.163	549	24.6	0.232894	9	3.23344	6.1	2.5	5.163	549.5	8.6	0.212354
2	5.591	742	33.2	0.0933722	10	1.57913	3	1.2	5.591	742.4	10	0.0894833
3	6.563	1202	53.8	0.2203	9	2.81714	5.3	2.2	6.563	1199.3	8.6	0.1846
4	6.61	1217	54.5	0.72991	3	3.97793	7.6	3.1	6.61	1217.3	3.1	0.733683
5	6.682	1240	55.5	0.208243	5	1.72198	3.3	1.3	6.682	1240.4	5.3	0.182693
6	6.77	1268	56.7	0.132955	7	1.72171	3.3	1.3	6.77	1268	7.2	0.134197
7	6.839	1290	57.7	0.559015	7	6.8715	13	5.4	6.839	1291.1	7.3	0.531154
8	7.034	1356	60.7	0.673207	7	7.92435	15	6.2	7.034	1358.8	6.9	0.645855
9	7.273	1437	64.3	0.0930243	8	1.26133	2.4	1	7.273	1437.9	8.2	0.0862989
10	7.466	1505	67.3	0.221006	10	3.80856	7.2	3	7.466	1504.1	10.2	0.21003
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.596	1552	69.4	0.528673	10	8.45178	16.1	6.6	7.596	1551.2	10	0.475932
12	7.943	1678	75.1	0.559113	9	9.28873	17.6	7.2	7.943	1677.8	9.3	0.56589

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	65	2.9	0.427131	20	14.2382	17.2	11.4	3.5	68.2	19.9	0.404351
2	4.55	382	17.2	0.497835	7	5.84385	7.1	4.7	4.55	382.6	7.1	0.462317
3	5.2	560	25.1	0.670693	9	9.70889	11.8	7.8	5.2	559.6	8.6	0.636558
4	5.85	861	38.7	0.796273	6	7.9767	9.7	6.4	5.85	861.2	5.5	0.813799
5	6.55	1199	53.8	0.571853	9	8.75054	10.6	7	6.55	1198.5	9.3	0.530176
6	6.795	1284	57.7	0.379792	10	6.47115	7.8	5.2	6.795	1283	10.1	0.360539
7	6.85	1303	58.5	0.0845959	6	0.928195	1.1	0.7	6.85	1302.6	6.5	0.0806109
8	7.35	1465	65.8	0.551862	11	10.065	12.2	8	7.35	1461.9	10.8	0.525146
9	8.15	1755	78.8	0.170153	18	5.40055	6.5	4.3	8.15	1756.6	18.2	0.167399
10	8.45	1871	84	0.277593	12	5.79258	7	4.6	8.45	1871	12	0.27198
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	8.65	1940	87.1	0.268368	8	3.82167	4.6	3.1	8.65	1940	8.2	0.263327
12	9.3	2192	98.4	0.335562	6	3.57413	4.3	2.9	9.3	2191.9	6.2	0.326694

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	83	3.7	0.399482	18	11.8184	8.3	6	3.5	85.2	17.7	0.376141
2	4.55	414	18.5	0.328797	12	6.18709	4.4	3.1	4.55	412.5	11.8	0.295213
3	5.073	558	24.9	0.207462	18	5.97428	4.2	3	5.073	556.4	18.1	0.186536
4	5.2	593	26.5	0.38015	11	7.29367	5.1	3.7	5.2	591.1	11	0.375368
5	5.552	751	33.5	0.109657	11	1.90342	1.3	1	5.552	749.9	10.6	0.101181
6	5.85	885	39.5	0.645857	6	7.10635	5	3.6	5.85	884.9	6.3	0.638037
7	6.55	1215	54.2	0.41548	9	6.37864	4.5	3.2	6.55	1213.8	9.1	0.395851
8	6.607	1231	54.9	0.191115	6	1.79597	1.3	0.9	6.607	1231	6.3	0.161197
9	6.671	1249	55.7	0.760237	3	4.42107	3.1	2.2	6.671	1248.5	3.4	0.7245
10	6.757	1273	56.8	0.164826	4	0.96548	0.7	0.5	6.757	1272.9	3.8	0.144427
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.85	1299	58	0.253502	8	3.3123	2.3	1.7	6.85	1298.3	7.7	0.243406
12	6.918	1323	59	0.506629	7	5.75972	4.1	2.9	6.918	1323.3	6.7	0.485027
13	7.114	1392	62.1	0.566435	9	8.37347	5.9	4.2	7.114	1394.2	8.8	0.536325
14	7.35	1475	65.8	0.342748	9	5.14063	3.6	2.6	7.35	1473.7	8.8	0.330916
15	7.526	1539	68.7	0.195725	13	4.19749	3	2.1	7.526	1537.5	12.6	0.18842
16	7.658	1587	70.8	0.481565	12	8.56609	6	4.3	7.658	1586.7	11.6	0.417694
17	8.015	1717	76.6	0.410754	10	7.24261	5.1	3.7	8.015	1717	10.1	0.406496
18	8.15	1766	78.8	0.122525	13	2.81467	2	1.4	8.15	1766.5	12.8	0.123764
19	8.45	1890	84.3	0.30663	36	16.9996	12	8.6	8.45	1894.2	36	0.266421
20	8.65	1959	87.4	0.364692	36	21.0186	14.8	10.6	8.65	1953	36	0.329409
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.3	2211	98.7	0.403157	6	4.50428	3.2	2.3	9.3	2210.8	6.2	0.407915

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.091	550	24.6	0.190316	10	3.10357	6.8	2.6	5.091	550.8	10	0.174613
2	5.561	747	33.4	0.0595169	10	1.01433	2.2	0.8	5.561	747.1	9.5	0.0601227
3	6.554	1214	54.3	0.128523	8	1.47393	3.2	1.2	6.554	1212	7.5	0.110253
4	6.61	1230	55	0.723644	3	3.76441	8.2	3.1	6.61	1229.9	2.9	0.721526
5	6.691	1253	56	0.112122	5	0.953983	2.1	0.8	6.691	1253.5	5.4	0.100592
6	6.794	1282	57.3	0.0719901	7	0.910796	2	0.8	6.794	1283.3	7.2	0.0711022
7	6.864	1303	58.3	0.567988	6	6.02978	13.2	5	6.864	1303	6	0.56871
8	7.092	1385	61.9	0.622977	9	9.54665	20.9	8	7.092	1385.2	8.6	0.622787
9	7.278	1452	64.9	0.0432938	9	0.795516	1.7	0.7	7.278	1447.3	8.5	0.0526151
10	7.47	1522	68.1	0.171973	12	3.34298	7.3	2.8	7.47	1519.4	12.1	0.155419
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.593	1567	70.1	0.453919	9	6.77575	14.8	5.6	7.593	1566.8	9.4	0.405475
12	7.944	1695	75.8	0.495053	9	7.98212	17.5	6.7	7.944	1694.5	8.9	0.504182

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	64	2.9	0.475209	27	20.4266	21.1	14.6	3.5	67.1	26.7	0.432015
2	4.55	381	17.1	0.555927	7	6.33507	6.5	4.5	4.55	381.1	6.9	0.521498
3	5.2	560	25.2	0.708529	8	10.0899	10.4	7.2	5.2	559.2	8.3	0.683335
4	5.85	869	39.1	0.766428	6	7.96166	8.2	5.7	5.85	869	5.7	0.786926
5	6.55	1204	54.1	0.577161	9	8.92139	9.2	6.4	6.55	1203.2	9.4	0.534085
6	6.85	1290	58	0.355742	11	6.42777	6.6	4.6	6.85	1289.6	11	0.329935
7	7.35	1474	66.3	0.551275	11	9.97738	10.3	7.1	7.35	1471.4	10.7	0.525433
8	8.15	1768	79.5	0.218331	18	6.85769	7.1	4.9	8.15	1767.2	18.1	0.213441
9	8.45	1879	84.5	0.424535	13	9.70045	10	6.9	8.45	1879.8	13.4	0.408823
10	8.65	1949	87.6	0.3464	10	5.79249	6	4.1	8.65	1948	9.8	0.334782
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2191	98.5	0.426637	6	4.37087	4.5	3.1	9.3	2191	6.3	0.393123

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 80

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 18.700 4.550 6.100 5.200 9.100 5.850 10.200 6.550 9.400 6.850 8.100 7.350 10.900 8.150 4.600 8.450 4.900 8.650 3.800 8.991 3.500 9.300 10.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 12 Return to Lane Navigator
5.163 6.100 5.591 3.000 6.563 5.300 6.610 7.600 6.682 3.300 6.770 3.300 6.839 13.000 7.034 15.000 7.273 2.400 7.466 7.200 7.596 16.100 7.943 17.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 12 Return to Lane Navigator
3.500 17.200 4.550 7.100 5.200 11.800 5.850 9.700 6.550 10.600 6.795 7.800 6.850 1.100 7.350 12.200 8.150 6.500 8.450 7.000 8.650 4.600 9.300 4.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 12 Return to Lane Navigator
3.500 8.300 4.550 4.400 5.073 4.200 5.200 5.100 5.552 1.300 5.850 5.000 6.550 4.500 6.607 1.300 6.671 3.100 6.757 0.700 6.850 2.300 6.918 4.100 7.114 5.900 7.350 3.600 7.526 3.000 7.658 6.000 8.015 5.100 8.150 2.000 8.450 12.000 8.650 14.800 9.300 3.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 21 Return to Lane Navigator
5.091 6.800 5.561 2.200 6.554 3.200 6.610 8.200 6.691 2.100 6.794 2.000 6.864 13.200 7.092 20.900 7.278 1.700 7.470 7.300 7.593 14.800 7.944 17.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 12 Return to Lane Navigator
3.500 21.100 4.550 6.500 5.200 10.400 5.850 8.200 6.550 9.200 6.850 6.600 7.350 10.300 8.150 7.100 8.450 10.000 8.650 6.000 9.300 4.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Skipjack Tuna ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Skipjack Tuna ---- Go to Display Gel Lane Navigator.