RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Sheepshead ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Archosargus probatocephalus
FDA Seafood List Common Name: Sheepshead
FDA Seafood List Market Name: Sheepshead
Unique IEF Gel Code: 19970205gel06
Unique Web Code: shh01Srr
Tissue Aliquot Code: archprob K96-005 #04m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	84	10.1	0.0755538	4	0.471278	5	3.6	4.55	84.2	3.6	0.072851
2	5.2	182	21.9	0.0840555	4	0.629315	6.6	4.8	5.2	182.1	4.4	0.0807511
3	5.85	298	35.9	0.0600906	3	0.309959	3.3	2.4	5.85	298	2.9	0.0600459
4	6.55	430	51.8	0.158312	6	1.62344	17.1	12.5	6.55	430	6	0.1532
5	6.85	462	55.7	0.0738933	5	0.593931	6.3	4.6	6.85	463	4.8	0.0699449
6	7.35	529	63.7	0.122817	4	0.832118	8.8	6.4	7.35	528.8	3.9	0.12098
7	8.15	658	79.3	0.138373	7	1.66279	17.6	12.8	8.15	658.5	6.9	0.135866
8	8.45	725	87.3	0.172732	7	2.0355	21.5	15.6	8.45	724.7	6.9	0.167626
9	8.65	773	93.1	0.148796	4	1.03933	11	8	8.65	773.1	4	0.146593
10	9.3	821	98.9	0.0519801	3	0.271881	2.9	2.1	9.3	820.8	2.9	0.0523712

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.213	31	3.7	0.256811	7	3.27538	6.8	5.2	4.213	30.4	7.2	0.256976
2	4.32	47	5.7	0.349121	5	3.06252	6.4	4.9	4.32	46.7	5	0.346175
3	4.513	76	9.2	0.0581074	7	0.661841	1.4	1.1	4.513	76	6.7	0.0555028
4	4.94	140	16.9	0.0255254	6	0.21254	0.4	0.3	4.94	140.1	5.9	0.0204902
5	5.107	165	19.9	0.0498302	5	0.396804	0.8	0.6	5.107	164.8	4.9	0.0452824
6	5.255	189	22.8	0.0846989	3	0.470011	1	0.8	5.255	188.8	3.2	0.08412
7	5.337	204	24.6	0.546229	4	3.70106	7.7	5.9	5.337	204.7	4.3	0.481595
8	5.43	221	26.6	0.02543	3	0.125666	0.3	0.2	5.43	222.2	2.9	0.0247083
9	5.474	229	27.6	0.0452056	2	0.206271	0.4	0.3	5.474	229.3	2.5	0.0466888
10	5.562	245	29.5	0.050231	2	0.0857296	0.2	0.1	5.562	245.2	1.7	0.0287075
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.606	253	30.5	0.0559024	7	0.653544	1.4	1	5.606	251.5	7.4	0.049549
12	5.732	276	33.3	0.503823	3	2.79541	5.8	4.5	5.732	275.8	3.3	0.479784
13	5.82	292	35.2	0.245927	4	1.5476	3.2	2.5	5.82	291.4	3.8	0.227098
14	5.879	303	36.5	0.124745	3	0.723736	1.5	1.2	5.879	302.5	3.3	0.124172
15	5.947	316	38.1	0.091644	3	0.40407	0.8	0.6	5.947	315.3	2.9	0.0794717
16	5.974	321	38.7	0.165243	3	0.89894	1.9	1.4	5.974	320.7	3	0.16685
17	6.268	377	45.4	0.0895153	8	1.12742	2.3	1.8	6.268	375.8	8.2	0.0773709
18	6.395	401	48.3	0.13573	5	1.12864	2.3	1.8	6.395	400.7	4.5	0.141083
19	6.432	408	49.2	0.344843	3	1.73366	3.6	2.8	6.432	408.6	2.8	0.352398
20	6.511	423	51	0.0900852	4	0.651192	1.4	1	6.511	423.5	4.2	0.0878055
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.547	430	51.8	0.257592	2	0.948153	2	1.5	6.547	429.6	2.1	0.25024
22	6.644	441	53.1	0.525694	2	2.20317	4.6	3.5	6.644	440.8	2.5	0.502606
23	6.769	455	54.8	0.191312	10	2.97526	6.2	4.8	6.769	455.5	10.2	0.164183
24	6.935	475	57.2	0.540612	4	3.62052	7.5	5.8	6.935	475.2	3.7	0.549828
25	7.065	492	59.3	0.53794	3	2.87759	6	4.6	7.065	492.3	3	0.544422
26	7.235	514	61.9	0.0582238	9	0.725425	1.5	1.2	7.235	511.7	9	0.0453772
27	7.312	524	63.1	0.0239719	1	0.0146685	0	0	7.312	524.5	1.3	0.006213
28	7.387	535	64.5	0.0389979	6	0.332416	0.7	0.5	7.387	535.8	6	0.0311461
29	7.469	548	66	0.39208	3	2.31309	4.8	3.7	7.469	548	3.4	0.388107
30	8.74	780	94	0.275761	18	8.32296	17.3	13.3	8.74	781.3	18	0.260879

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	79	9.5	0.0506168	4	0.34368	4.4	3.2	4.55	78.8	3.9	0.0492655
2	5.2	176	21.2	0.06215	5	0.505397	6.5	4.6	5.2	176.1	4.9	0.0586537
3	5.85	297	35.8	0.0443509	3	0.238596	3.1	2.2	5.85	297.3	3.1	0.0430932
4	6.55	431	51.9	0.119057	6	1.2609	16.2	11.6	6.55	430.7	6.4	0.110726
5	6.85	466	56.1	0.0290458	6	0.287718	3.7	2.6	6.85	467	6.2	0.0261097
6	7.35	529	63.7	0.0733206	4	0.49722	6.4	4.6	7.35	528.9	4	0.0699064
7	8.15	656	79	0.119204	7	1.45536	18.7	13.3	8.15	655.4	7.2	0.114453
8	8.45	726	87.5	0.119303	7	1.40718	18.1	12.9	8.45	725.1	6.8	0.117111
9	8.65	774	93.3	0.11844	4	0.843162	10.8	7.7	8.65	773.5	4.2	0.113207
10	9.3	820	98.8	0.0930813	7	0.948765	12.2	8.7	9.3	817.9	6.7	0.079506

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.225	33	4	0.258953	6	2.65844	6.9	5.5	4.225	32.8	5.9	0.253045
2	4.313	47	5.7	0.317291	5	2.79777	7.2	5.8	4.313	46.6	5.1	0.311069
3	4.444	68	8.2	0.0228763	7	0.339797	0.9	0.7	4.444	72	6.9	0.0278866
4	4.55	85	10.2	0.0405547	3	0.189822	0.5	0.4	4.55	84.7	3	0.0352256
5	4.9	141	17	0.0158519	5	0.102953	0.3	0.2	4.9	142	4.6	0.0127169
6	5	157	18.9	0.0215865	6	0.189158	0.5	0.4	5	156.1	5.5	0.0192381
7	5.069	168	20.2	0.0183284	2	0.0629628	0.2	0.1	5.069	167.9	2	0.0173885
8	5.2	189	22.7	0.0767101	5	0.748955	1.9	1.6	5.2	187.5	5.5	0.0768371
9	5.285	204	24.5	0.504949	4	3.43312	8.9	7.1	5.285	205.5	4.2	0.459673
10	5.381	221	26.6	0.018414	2	0.0790412	0.2	0.2	5.381	221.9	2.2	0.0199613
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.426	229	27.6	0.0297053	3	0.161753	0.4	0.3	5.426	228.7	2.9	0.0316008
12	5.522	246	29.6	0.0407142	2	0.0557214	0.1	0.1	5.522	245.6	2	0.0158666
13	5.55	251	30.2	0.0467911	8	0.584668	1.5	1.2	5.55	251.2	7.9	0.0415338
14	5.686	275	33.1	0.488679	3	2.77974	7.2	5.8	5.686	275.3	3.4	0.462368
15	5.777	291	35	0.268126	5	2.24141	5.8	4.6	5.777	290.6	5.5	0.230474
16	5.85	304	36.6	0.0952756	4	0.635069	1.6	1.3	5.85	304	4	0.0895215
17	5.899	313	37.7	0.108933	4	0.905366	2.3	1.9	5.899	314.4	4.4	0.116929
18	5.932	319	38.4	0.173023	3	0.718983	1.9	1.5	5.932	319.5	3	0.137439
19	6.232	374	45	0.0755843	7	0.878883	2.3	1.8	6.232	372.8	7.3	0.0680477
20	6.379	401	48.3	0.147687	4	1.01842	2.6	2.1	6.379	399.8	4.1	0.140379
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.417	408	49.1	0.328909	3	1.52907	4	3.2	6.417	407.9	2.6	0.326423
22	6.488	421	50.7	0.0752865	5	0.686687	1.8	1.4	6.488	423	4.6	0.0837753
23	6.532	429	51.6	0.245178	2	0.825115	2.1	1.7	6.532	428.6	2	0.236686
24	6.609	439	52.8	0.454796	2	1.81404	4.7	3.8	6.609	439.6	2.2	0.474048
25	6.724	452	54.4	0.144165	5	1.24236	3.2	2.6	6.724	452.2	5.1	0.13623
26	6.812	462	55.6	0.0327783	3	0.1873	0.5	0.4	6.812	463.2	2.7	0.0387848
27	6.919	475	57.2	0.548671	3	2.60615	6.7	5.4	6.919	474.6	2.7	0.554806
28	7.071	494	59.4	0.517147	2	2.37174	6.1	4.9	7.071	493.7	2.5	0.536521
29	7.238	515	62	0.0528798	5	0.39552	1	0.8	7.238	514	4.5	0.0495186
30	7.35	529	63.7	0.0909937	7	1.09358	2.8	2.3	7.35	530	7.2	0.086025
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.46	547	65.8	0.30817	3	1.73131	4.5	3.6	7.46	547	3.2	0.301968
32	8.15	660	79.4	0.0176404	6	0.29164	0.8	0.6	8.15	656.4	5.8	0.0282947
33	8.45	726	87.4	0.0523714	5	0.459597	1.2	1	8.45	726.8	4.8	0.0543576
34	8.65	775	93.3	0.137096	13	2.80875	7.3	5.8	8.65	777.6	12.5	0.126773

PeakCounter: 34

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.192	31	3.7	0.269152	6	2.698	6.9	5.4	4.192	31.2	5.9	0.25794
2	4.285	45	5.4	0.342184	5	2.90312	7.5	5.8	4.285	45.4	4.9	0.33769
3	4.437	68	8.2	0.0512658	12	0.874088	2.2	1.8	4.437	69.4	12	0.0412202
4	4.882	135	16.2	0.0252986	9	0.294534	0.8	0.6	4.882	134.9	8.6	0.0192612
5	5.041	159	19.1	0.0315593	3	0.177864	0.5	0.4	5.041	158.8	3.5	0.0290265
6	5.187	181	21.8	0.0686932	3	0.308175	0.8	0.6	5.187	181	2.7	0.0644384
7	5.278	197	23.7	0.505785	5	3.92151	10.1	7.9	5.278	198.2	5	0.444412
8	5.384	216	26	0.0257286	3	0.115719	0.3	0.2	5.384	216.8	2.6	0.0250315
9	5.445	227	27.3	0.0387763	3	0.212599	0.5	0.4	5.445	226.4	3.4	0.0357968
10	5.535	243	29.2	0.0494589	3	0.232959	0.6	0.5	5.535	242.9	3	0.0443395
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.568	249	30	0.0438426	4	0.288096	0.7	0.6	5.568	248.8	3.9	0.0419468
12	5.685	270	32.5	0.473216	3	2.61016	6.7	5.3	5.685	269.7	3.1	0.474578
13	5.758	283	34.1	0.22948	4	1.42554	3.7	2.9	5.758	282.3	3.8	0.213616
14	5.808	292	35.1	0.120495	3	0.580503	1.5	1.2	5.808	292	2.7	0.119652
15	5.863	302	36.3	0.0903004	3	0.448948	1.2	0.9	5.863	302.9	2.6	0.0968144
16	5.895	308	37.1	0.179141	3	0.924948	2.4	1.9	5.895	308.1	3	0.175887
17	6.215	369	44.4	0.0947627	7	0.953352	2.4	1.9	6.215	368.4	7	0.0773636
18	6.352	395	47.5	0.1173	3	0.611631	1.6	1.2	6.352	394.1	3.2	0.108707
19	6.368	398	47.9	0.135755	2	0.468534	1.2	0.9	6.368	398.6	2.2	0.118976
20	6.41	406	48.9	0.37059	3	1.86505	4.8	3.8	6.41	406.2	2.8	0.37681
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.489	421	50.7	0.0922769	4	0.69459	1.8	1.4	6.489	421.7	4.2	0.0926317
22	6.52	427	51.4	0.258954	2	0.922823	2.4	1.9	6.52	427.5	2	0.258124
23	6.598	438	52.7	0.525615	2	2.13399	5.5	4.3	6.598	438.3	2.4	0.5105
24	6.717	451	54.3	0.166865	5	1.38974	3.6	2.8	6.717	450.7	5.1	0.152451
25	6.798	460	55.4	0.0353494	2	0.106968	0.3	0.2	6.798	460.4	1.9	0.0318648
26	6.893	471	56.7	0.549567	3	2.75491	7.1	5.5	6.893	471.3	2.8	0.55697
27	7.048	490	59	0.538039	3	2.58056	6.6	5.2	7.048	490	2.7	0.54337
28	7.22	511	61.5	0.0738514	7	0.774184	2	1.6	7.22	509.1	6.8	0.0639588
29	7.301	521	62.7	0.035068	3	0.163129	0.4	0.3	7.301	521.2	2.7	0.0335098
30	7.375	531	63.9	0.0287168	2	0.127283	0.3	0.3	7.375	530.7	2.5	0.0292667
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.442	542	65.2	0.367358	3	2.05968	5.3	4.1	7.442	542	3.1	0.369219
32	8.671	774	93.1	0.114637	18	3.30115	8.5	6.6	8.671	769.7	18	0.103473

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	85	10.2	0.0521699	3	0.308371	3.4	2.6	4.55	84.9	3.3	0.052897
2	5.2	177	21.3	0.0577094	6	0.576828	6.4	4.8	5.2	178.1	5.9	0.0556184
3	5.85	295	35.5	0.03747	4	0.263109	2.9	2.2	5.85	294.5	4.3	0.0348262
4	6.55	433	52.1	0.145019	6	1.43128	15.9	12	6.55	432.8	5.8	0.139027
5	6.85	465	56	0.0459152	5	0.367459	4.1	3.1	6.85	465.2	4.6	0.0448375
6	7.35	525	63.2	0.0940418	4	0.70461	7.8	5.9	7.35	525.2	4.3	0.0917852
7	8.15	654	78.7	0.129403	8	1.72881	19.2	14.5	8.15	653.7	7.6	0.127591
8	8.45	722	86.9	0.14336	7	1.64478	18.3	13.8	8.45	721.8	6.6	0.140726
9	8.65	770	92.7	0.115826	5	0.975378	10.8	8.2	8.65	769.3	4.8	0.113732
10	9.3	818	98.4	0.068551	9	1.00294	11.1	8.4	9.3	815.2	8.9	0.0637451

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 126

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.000 5.200 6.600 5.850 3.300 6.550 17.100 6.850 6.300 7.350 8.800 8.150 17.600 8.450 21.500 8.650 11.000 9.300 2.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.213 6.800 4.320 6.400 4.513 1.400 4.940 0.400 5.107 0.800 5.255 1.000 5.337 7.700 5.430 0.300 5.474 0.400 5.562 0.200 5.606 1.400 5.732 5.800 5.820 3.200 5.879 1.500 5.947 0.800 5.974 1.900 6.268 2.300 6.395 2.300 6.432 3.600 6.511 1.400 6.547 2.000 6.644 4.600 6.769 6.200 6.935 7.500 7.065 6.000 7.235 1.500 7.312 0 7.387 0.700 7.469 4.800 8.740 17.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 30 Return to Lane Navigator
4.550 4.400 5.200 6.500 5.850 3.100 6.550 16.200 6.850 3.700 7.350 6.400 8.150 18.700 8.450 18.100 8.650 10.800 9.300 12.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.225 6.900 4.313 7.200 4.444 0.900 4.550 0.500 4.900 0.300 5.000 0.500 5.069 0.200 5.200 1.900 5.285 8.900 5.381 0.200 5.426 0.400 5.522 0.100 5.550 1.500 5.686 7.200 5.777 5.800 5.850 1.600 5.899 2.300 5.932 1.900 6.232 2.300 6.379 2.600 6.417 4.000 6.488 1.800 6.532 2.100 6.609 4.700 6.724 3.200 6.812 0.500 6.919 6.700 7.071 6.100 7.238 1.000 7.350 2.800 7.460 4.500 8.150 0.800 8.450 1.200 8.650 7.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 34 Return to Lane Navigator
4.192 6.900 4.285 7.500 4.437 2.200 4.882 0.800 5.041 0.500 5.187 0.800 5.278 10.100 5.384 0.300 5.445 0.500 5.535 0.600 5.568 0.700 5.685 6.700 5.758 3.700 5.808 1.500 5.863 1.200 5.895 2.400 6.215 2.400 6.352 1.600 6.368 1.200 6.410 4.800 6.489 1.800 6.520 2.400 6.598 5.500 6.717 3.600 6.798 0.300 6.893 7.100 7.048 6.600 7.220 2.000 7.301 0.400 7.375 0.300 7.442 5.300 8.671 8.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 32 Return to Lane Navigator
4.550 3.400 5.200 6.400 5.850 2.900 6.550 15.900 6.850 4.100 7.350 7.800 8.150 19.200 8.450 18.300 8.650 10.800 9.300 11.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Sheepshead ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Sheepshead ---- Go to Display Gel Lane Navigator.