Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Quillback Rockfish  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Sebastes maliger
FDA Seafood List Common Name: Quillback Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19940913gel01
Unique Web Code: qbg01Srr
Tissue Aliquot Code: sebamali I94-002 #06a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel ImageCalibrated Comparison Gel Image
Gel ImageFish ImageCalibrated GelComparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane z
pI Markers
Lane d
Specimen
Lane e
Co-Migrated
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 18
LANE TYPE: pI Markers
ACTUAL GEL LANE: 20
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5504.30.150899163.7801617.713.93.547.115.70.135465
24.5519917.30.2719652.13147107.84.55198.94.80.248103
35.227924.20.35959842.4892111.79.15.227940.349817
45.85427370.46107232.5056511.89.25.85426.830.468367
56.5559451.50.30621142.1477610.17.96.55593.84.20.291026
66.85634550.087325980.9882574.63.66.85634.77.50.0740571
77.35726630.28338842.064769.77.67.35725.24.20.276915
88.1588576.80.05781290.9435094.43.58.158859.30.0573418
98.4595682.90.082593970.9489384.53.58.45955.76.90.077789
108.6599986.60.069841350.63519732.38.65998.75.30.0675221
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3112997.90.37146642.6760412.69.89.31127.64.50.337947
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 17
LANE TYPE: Specimen
ACTUAL GEL LANE: 19
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.13413611.80.51097443.8655916.613.14.134136.14.20.523878
24.46518315.90.026136660.23465910.84.465181.55.60.0235173
34.69421318.50.5911844.1275417.7144.694213.23.80.607262
45.00525221.90.16031171.808847.86.15.005254.86.90.148417
55.69138933.80.070263880.7781453.32.65.691388.87.60.0578342
65.76140535.20.034964770.432781.91.55.7614067.20.0338902
75.8642837.20.55292232.55168118.75.86427.92.60.556981
85.96145339.30.26195341.762177.665.961453.24.10.239899
96.0547541.20.068809430.3663761.61.26.05475.33.20.0638708
106.08248341.90.014417410.0419580.20.16.0824841.50.0162521
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.1149042.50.027678720.08017220.30.36.11489.91.60.0280517
126.15150043.40.35520621.3900164.76.151500.52.10.37789
136.37255548.20.044931270.4304381.91.56.372554.66.60.0367014
146.48958450.70.025589940.1608790.70.56.489583.93.90.0231454
156.53859651.70.053097860.4979332.11.76.538595.85.80.0484523
167.65979168.70.033054440.212220.90.77.659790.83.90.0308212
178.49296483.70.129257204.5184919.415.38.492964.820.20.126302
PeakCounter: 17
DISPLAY GEL LANE LETTER: Lane z
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5433.70.13127163.533141713.53.540.416.30.122447
24.5519216.70.21877662.009389.77.74.55192.75.50.205981
35.227523.90.27884652.3538411.495.2275.14.80.274952
45.8542536.90.39058542.72113.110.45.85424.53.90.38936
56.5560552.50.29784342.018999.77.76.55605.240.2879
66.8564455.90.069911870.7328633.52.86.85643.46.70.0620285
77.3573563.80.23885341.850458.97.17.35734.84.50.234319
88.1589277.40.0470594110.8525814.13.28.15894.210.60.0453693
98.45956830.07673670.907094.43.58.45956.66.60.077522
108.6599486.30.066065660.6879573.32.68.65994.86.20.0631031
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3113098.10.35349653.0678714.811.79.31129.35.30.324342
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.13413411.60.51345443.9127914.111.84.1341344.20.5219
24.43517715.40.027700750.217570.80.74.435176.94.70.0259314
34.67120918.20.4868554.0520314.612.24.671208.64.60.493497
44.98524921.60.109206164.0370714.612.24.985259.316.40.13891
55.71339434.20.0368694130.7603392.72.35.713393.513.10.0326698
65.76940735.40.024963550.1412770.50.45.769409.25.30.01516
75.8642837.20.3948742.8289910.28.55.864294.10.393429
85.96845539.50.27236552.395268.77.25.968455.25.50.245971
96.05647741.40.028020830.1811030.70.56.056477.83.30.0306928
106.08448442.10.024983520.08356030.30.36.084484.32.10.0225934
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.11649242.70.023129920.0881370.30.36.116491.31.90.0261398
126.15650243.60.26281631.174874.23.56.156501.92.50.263108
136.3855848.50.053045470.56463821.76.38556.47.20.044532
146.496587510.021710840.1235390.40.46.496586.33.80.01816
156.54598520.051869170.5915682.11.86.54598.66.50.0513131
167.37573663.90.014602440.09677790.30.37.375735.140.0135044
177.65879268.80.042433140.3333381.217.658791.34.50.0421416
188.46295382.80.138439256.098822218.48.462948.324.80.138587
PeakCounter: 18
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5383.30.0821139141.716384.33.73.537.613.50.0715731
24.12612811.20.49968143.895759.68.44.126128.64.30.509164
34.41817014.90.03978660.40234810.94.418171.25.90.0383149
44.5518916.50.13170761.255573.12.74.55188.35.70.125253
54.65220217.70.4593153.883959.68.44.652202.24.70.465439
64.97324321.20.104048183.463948.67.54.973254.617.60.110892
75.227223.80.18339651.2089632.65.2271.850.13717
85.67438033.20.059906960.588111.51.35.674380.56.40.0522314
95.762400350.021926590.38470210.85.762395.790.0241646
105.8542036.70.47976143.464318.67.55.85419.94.20.460459
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.96144739.10.28814762.908937.26.35.961446.16.40.25629
126.05647041.10.067665330.3315070.80.76.056470.12.90.0640821
136.11848542.40.039568170.4270221.10.96.118482.27.50.0321888
146.16349643.40.29787931.360013.42.96.163496.12.50.305915
156.39855348.30.027588640.1848890.50.46.3985533.70.0280189
166.51758250.90.082836481.024242.52.26.517583.37.90.0729507
176.5559051.60.12655250.8381232.11.86.55591.55.20.0913675
186.8563155.20.057716950.4835981.216.85630.45.30.0517547
197.35721630.22833841.713074.23.77.35720.44.30.222657
207.42573664.30.016517650.1134820.30.27.425735.44.60.0138499
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.779169.10.034096850.2950380.70.67.7790.650.0334735
228.15881770.48367532.13535.34.68.15880.42.60.455006
238.4593982.10.139201245.248281311.48.45931.6240.123378
248.65984860.046887440.2748950.70.68.659854.10.0377189
259.3111697.60.2877662.776276.969.31115.15.70.275942
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5474.10.146893143.230215.512.33.546.213.90.130646
24.55195170.23596751.937319.37.44.55195.24.90.222147
35.227824.20.25474252.4621111.89.45.22775.40.258435
45.85426370.36920242.4755811.99.45.85425.83.70.373581
56.5559651.80.27826642.012359.77.66.55595.84.30.261296
66.8563655.30.081331870.9520324.63.66.85635.97.30.073651
77.3572763.20.28329542.1880110.58.37.35726.64.50.275249
88.15886770.0595528111.129365.44.38.15885.511.50.0555628
98.4594982.50.084279381.107935.34.28.459507.60.0827929
108.6599086.10.063301460.6473783.12.58.65989.95.90.0622323
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3112497.70.29959952.6375712.7109.31123.15.40.277838
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b z d e f
MK SP MK SP CO MK
white spacer for gel schematic
pI 3.50 label for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 3.50 label for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 93
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 18
LANE TYPE: pI Markers
ACTUAL GEL LANE: 20
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 17
LANE TYPE: Specimen
ACTUAL GEL LANE: 19
PeakCount: 17 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane z
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
PeakCount: 18 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
PeakCount: 11 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


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A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

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Last updated on 2003-FEB-27 by bjt/frf/nln