RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus macrocephalus
FDA Seafood List Common Name: Pacific Cod
FDA Seafood List Market Name: Cod (Alaska Cod)
Unique IEF Gel Code: 19930816gel01
Unique Web Code: pcg02Srr
Tissue Aliquot Code: gadumacr 003 #2 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	60	5.1	0.260332	19	8.04519	30.8	26.3	3.5	53.2	19.2	0.236126
2	4.55	207	17.5	0.360163	3	1.86285	7.1	6.1	4.55	206.7	3.1	0.336917
3	5.2	287	24.3	0.410366	4	2.54981	9.8	8.3	5.2	286.8	3.6	0.396662
4	5.85	428	36.2	0.41107	3	1.90092	7.3	6.2	5.85	428.2	2.6	0.410684
5	6.55	601	50.9	0.303896	4	2.31145	8.8	7.6	6.55	600.2	4.4	0.293134
6	6.85	638	54	0.0655465	6	0.605878	2.3	2	6.85	638.6	5.8	0.0589054
7	7.35	734	62.2	0.229867	5	1.88408	7.2	6.2	7.35	733.3	4.9	0.21582
8	8.15	894	75.7	0.0546466	9	0.84412	3.2	2.8	8.15	893.7	9.1	0.0523628
9	8.45	960	81.3	0.0702827	6	0.752282	2.9	2.5	8.45	960.2	6.1	0.0692491
10	8.65	1001	84.8	0.0591252	5	0.529738	2	1.7	8.65	1000.5	5.1	0.0583328
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1127	95.4	0.336464	9	4.85316	18.6	15.9	9.3	1124.7	8.8	0.312087

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	62	5.3	0.236777	15	5.82441	11.6	9.3	3.5	59.3	15	0.219685
2	3.704	91	7.7	0.103298	9	1.64825	3.3	2.6	3.704	89.9	8.9	0.104221
3	4.303	176	14.9	0.509048	4	3.50856	7	5.6	4.303	175.7	3.9	0.513614
4	4.487	202	17.1	0.0763168	7	0.903324	1.8	1.4	4.487	201.8	7.3	0.0696085
5	4.55	211	17.9	0.227864	5	1.57582	3.1	2.5	4.55	210.3	4.6	0.194637
6	4.658	224	19	0.0145884	4	0.198078	0.4	0.3	4.658	220.5	4.2	0.0268652
7	4.825	244	20.7	0.130308	6	1.10677	2.2	1.8	4.825	243.1	5.6	0.111344
8	4.942	258	21.9	0.00710838	2	0.0205415	0	0	4.942	259.3	2	0.00592637
9	5.092	276	23.4	0.110486	7	1.34414	2.7	2.2	5.092	274.8	7.4	0.101803
10	5.2	289	24.5	0.221064	6	2.18988	4.4	3.5	5.2	288.5	6.2	0.200333
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.268	303	25.7	0.514541	4	3.20699	6.4	5.1	5.268	302.8	3.7	0.485033
12	5.321	314	26.6	0.31591	5	2.42694	4.8	3.9	5.321	314.2	4.8	0.283262
13	5.423	335	28.4	0.114221	3	0.642122	1.3	1	5.423	335.1	3.2	0.112146
14	5.535	358	30.3	0.0153233	2	0.0459598	0.1	0.1	5.535	357.8	1.7	0.0151857
15	5.583	368	31.2	0.00416545	1	0	0	0	5.583	362	1	0
16	5.632	378	32	0.574516	3	3.20422	6.4	5.1	5.632	378.7	3.3	0.545325
17	5.724	397	33.6	0.287733	5	2.27722	4.5	3.7	5.724	397.7	4.6	0.279837
18	5.85	423	35.8	0.120757	4	0.892867	1.8	1.4	5.85	422.9	4.3	0.116492
19	5.954	449	38.1	0.066875	11	1.12428	2.2	1.8	5.954	450.5	11	0.0574198
20	6.062	476	40.3	0.249413	2	1.04739	2.1	1.7	6.062	475.6	2.4	0.247881
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.102	486	41.2	0.00307961	1	0	0	0	6.102	466	1	0
22	6.142	496	42	0.149065	2	0.577125	1.1	0.9	6.142	496.1	2.2	0.151419
23	6.174	504	42.7	0.244246	2	0.930794	1.9	1.5	6.174	504.1	2.1	0.246791
24	6.222	516	43.7	0.0244139	6	0.234332	0.5	0.4	6.222	515.8	5.7	0.0233325
25	6.306	537	45.5	0.0728528	10	1.26622	2.5	2	6.306	536.9	10.1	0.0707063
26	6.414	564	47.8	0.0155913	7	0.136817	0.3	0.2	6.414	566.1	6.6	0.0116134
27	6.55	598	50.7	0.18115	5	1.34687	2.7	2.2	6.55	597.2	4.6	0.166749
28	6.85	636	53.9	0.0435047	3	0.235156	0.5	0.4	6.85	636.1	3.3	0.040336
29	7.35	731	61.9	0.282097	6	2.72782	5.4	4.4	7.35	729.7	5.9	0.261533
30	7.456	752	63.7	0.0434563	10	0.727868	1.4	1.2	7.456	751.3	9.9	0.0415078
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.15	889	75.3	0.619091	2	2.19833	4.4	3.5	8.15	889.7	2	0.618728
32	8.45	954	80.8	0.0745229	7	0.930888	1.9	1.5	8.45	954.1	7.3	0.0719806
33	8.65	998	84.6	0.0749047	5	0.604632	1.2	1	8.65	997.3	4.8	0.0717231
34	9.3	1134	96.1	0.393312	9	5.10508	10.2	8.2	9.3	1130.6	8.5	0.337617

PeakCounter: 34

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.807	113	9.6	0.0392626	13	0.872099	3.4	3	3.807	113.9	13	0.0378997
2	4.114	158	13.4	0.562688	5	5.36219	21.2	18.6	4.114	157.9	5.3	0.569164
3	4.284	183	15.5	0.421288	4	3.16627	12.5	11	4.284	182.6	4.2	0.427876
4	4.434	205	17.4	0.028328	5	0.222453	0.9	0.8	4.434	203.8	4.5	0.0277602
5	4.575	225	19.1	0.014928	7	0.203925	0.8	0.7	4.575	222.3	7.4	0.0155592
6	4.745	245	20.8	0.0498908	6	0.56372	2.2	2	4.745	246.4	6.1	0.0520572
7	4.915	265	22.5	0.0043459	6	0.111219	0.4	0.4	4.915	269.9	6	0.0104916
8	5.017	277	23.5	0.0479115	4	0.284604	1.1	1	5.017	276.7	3.5	0.0455114
9	5.085	285	24.2	0.0412384	3	0.245032	1	0.9	5.085	285.1	3.2	0.0433885
10	5.179	296	25.1	0.03596	5	0.587512	2.3	2	5.179	300.2	5.5	0.0605537
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.252	309	26.2	0.321211	5	2.42882	9.6	8.4	5.252	308.4	4.9	0.282484
12	5.306	320	27.1	0.182393	5	1.54487	6.1	5.4	5.306	320.3	5.1	0.172
13	5.424	344	29.2	0.0355629	5	0.287135	1.1	1	5.424	343.2	4.9	0.0333496
14	5.527	365	30.9	0.00219989	4	0.0201768	0.1	0.1	5.527	364.7	3.8	0.00301975
15	5.655	391	33.1	0.202986	5	1.91616	7.6	6.7	5.655	390.9	5.2	0.209191
16	5.754	411	34.8	0.0658437	4	0.3722	1.5	1.3	5.754	410.4	4.1	0.0509305
17	5.803	421	35.7	0.0537278	9	0.866489	3.4	3	5.803	421.2	8.7	0.0559968
18	5.931	450	38.1	0.1094	4	0.353787	1.4	1.2	5.931	447.8	4.3	0.0468308
19	5.964	458	38.8	0.0972617	12	1.9285	7.6	6.7	5.964	457.6	11.5	0.0945712
20	6.043	477	40.4	0.193823	1	0.482344	1.9	1.7	6.043	477.5	1.4	0.189503
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.121	496	42	0.10261	2	0.300163	1.2	1	6.121	496	1.6	0.103036
22	6.159	505	42.8	0.245532	1	0.638801	2.5	2.2	6.159	504.7	1.4	0.255943
23	6.196	514	43.6	0.00661662	3	0.039444	0.2	0.1	6.196	513	3.1	0.00712956
24	6.262	530	44.9	0.0416078	7	0.467966	1.8	1.6	6.262	530.8	6.8	0.03907
25	6.42	568	48.1	0.0103836	3	0.0659699	0.3	0.2	6.42	566.6	3.4	0.0108133
26	7.25	710	60.2	0.0323233	18	1.08594	4.3	3.8	7.25	713.5	18	0.0340381
27	7.436	746	63.2	0.0624596	10	0.917436	3.6	3.2	7.436	747.7	9.7	0.0535156

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	74	6.3	0.226155	22	7.96329	30.7	25.1	3.5	67	22.3	0.201607
2	4.55	233	19.7	0.28948	5	2.24163	8.6	7.1	4.55	232.5	4.7	0.269192
3	5.2	308	26.1	0.405459	4	2.74573	10.6	8.6	5.2	308.1	3.9	0.394737
4	5.85	438	37.1	0.426858	3	2.23778	8.6	7	5.85	438.1	3	0.417677
5	6.55	601	50.9	0.365305	4	2.71658	10.5	8.5	6.55	600.3	4.4	0.351454
6	6.85	636	53.9	0.0813771	4	0.501035	1.9	1.6	6.85	636.1	3.7	0.0761395
7	7.35	726	61.5	0.233125	5	1.93697	7.5	6.1	7.35	725.5	4.9	0.222796
8	8.15	895	75.8	0.048874	9	0.733293	2.8	2.3	8.15	894.5	8.7	0.0477254
9	8.45	962	81.5	0.0655885	6	0.674898	2.6	2.1	8.45	961.6	5.9	0.0649015
10	8.65	1004	85.1	0.0550144	6	0.506229	2	1.6	8.65	1003.5	5.6	0.0511537
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1138	96.4	0.367905	6	3.67095	14.2	11.5	9.3	1136.1	6.5	0.320738

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	79	6.7	0.266832	11	4.35728	8.1	7.2	3.5	80.3	10.6	0.23208
2	3.85	127	10.8	0.0630145	13	1.40103	2.6	2.3	3.85	126.7	13.3	0.0593856
3	4.142	167	14.2	0.624945	5	5.42757	10.1	9	4.142	166.6	4.8	0.64
4	4.317	191	16.2	0.478662	4	3.09775	5.7	5.1	4.317	191.1	3.7	0.476934
5	4.47	212	18	0.0349213	3	0.160476	0.3	0.3	4.47	210.7	2.9	0.0313131
6	4.55	223	18.9	0.247848	3	1.37357	2.5	2.3	4.55	223.6	3.3	0.23699
7	4.66	236	20	0.00332369	2	0.010118	0	0	4.66	236.5	1.7	0.0032625
8	4.744	246	20.9	0.0103395	3	0.0518003	0.1	0.1	4.744	244.8	2.8	0.0105261
9	4.837	257	21.8	0.0964084	3	0.386706	0.7	0.6	4.837	257.5	2.9	0.0750135
10	4.921	267	22.6	0.0517916	8	0.853019	1.6	1.4	4.921	266.9	8	0.0601434
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.014	278	23.6	0.112345	5	0.77826	1.4	1.3	5.014	277.8	4.5	0.0975739
12	5.09	287	24.3	0.0913199	2	0.310657	0.6	0.5	5.09	287.5	2.2	0.0803743
13	5.2	300	25.4	0.207388	3	1.29414	2.4	2.1	5.2	299.4	3.4	0.213883
14	5.246	310	26.3	0.543747	3	3.14116	5.8	5.2	5.246	310.3	3.2	0.551244
15	5.316	325	27.6	0.357486	3	1.5535	2.9	2.6	5.316	325.6	2.8	0.318305
16	5.414	346	29.3	0.0884725	3	0.471485	0.9	0.8	5.414	346	3	0.0881328
17	5.511	367	31.1	0.00940321	6	0.11855	0.2	0.2	5.511	367.7	6.3	0.0106088
18	5.548	375	31.8	0.0123862	1	0	0	0	5.548	360	1	0
19	5.604	387	32.8	0.665466	3	3.91137	7.3	6.5	5.604	387.6	3.4	0.646403
20	5.678	403	34.2	0.463935	5	3.52878	6.5	5.8	5.678	402.3	5	0.401594
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.771	423	35.9	0.0867066	7	1.05409	2	1.7	5.771	420.5	7.2	0.0822301
22	5.85	440	37.3	0.271512	3	1.2274	2.3	2	5.85	439.8	2.5	0.271916
23	5.965	466	39.5	0.0371561	3	0.231375	0.4	0.4	5.965	466.9	3.4	0.0380928
24	6.018	478	40.5	0.0156503	4	0.098728	0.2	0.2	6.018	478.3	4	0.0140815
25	6.054	486	41.2	0.307781	3	1.4975	2.8	2.5	6.054	486.1	2.7	0.316267
26	6.169	512	43.4	0.289869	3	1.80514	3.3	3	6.169	511.3	3.4	0.295842
27	6.297	541	45.9	0.0426375	8	0.543359	1	0.9	6.297	541.4	8	0.038218
28	6.417	568	48.2	0.041299	18	1.62724	3	2.7	6.417	582.1	18	0.0511029
29	6.55	598	50.7	0.206411	5	1.55915	2.9	2.6	6.55	597.1	5	0.174892
30	6.85	637	54	0.0845119	5	0.647678	1.2	1.1	6.85	637.8	4.7	0.0776183
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.35	728	61.7	0.269143	7	3.12117	5.8	5.1	7.35	727	7.1	0.249581
32	7.463	751	63.7	0.0429303	10	0.737343	1.4	1.2	7.463	750.7	9.9	0.0420567
33	8.15	891	75.6	0.261518	6	2.26908	4.2	3.7	8.15	891.2	5.6	0.228341
34	8.45	954	80.9	0.0644931	6	0.632857	1.2	1	8.45	954.4	6.2	0.0572212
35	8.65	1000	84.8	0.0507905	5	0.481127	0.9	0.8	8.65	999.1	5.4	0.0505116
36	9.3	1139	96.6	0.414349	7	4.13124	7.7	6.8	9.3	1136.4	6.8	0.344519

PeakCounter: 36

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.747	115	9.8	0.07497	8	1.08276	3.3	3	3.747	115.8	8.1	0.0750004
2	4.103	164	13.9	0.551973	6	5.60743	16.9	15.7	4.103	163.8	5.6	0.56211
3	4.307	192	16.3	0.316963	5	3.12073	9.4	8.7	4.307	191.7	5.4	0.325906
4	4.466	214	18.2	0.0208391	6	0.197057	0.6	0.6	4.466	213.9	5.5	0.0201172
5	4.631	235	19.9	0.0112413	7	0.130454	0.4	0.4	4.631	235.2	7	0.0104992
6	4.869	263	22.3	0.0315762	7	0.369736	1.1	1	4.869	257.7	7.4	0.0282534
7	4.988	277	23.5	0.0146406	2	0.0488287	0.1	0.1	4.988	276.7	2.1	0.0133766
8	5.064	286	24.3	0.0161908	4	0.113936	0.3	0.3	5.064	285.6	4.2	0.0151546
9	5.264	316	26.8	0.223259	10	3.53565	10.7	9.9	5.264	312.6	9.5	0.209855
10	5.327	330	28	0.145468	5	1.16875	3.5	3.3	5.327	329.6	5.1	0.128639
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.409	348	29.5	0.0356881	3	0.216655	0.7	0.6	5.409	347.8	3.4	0.0356859
12	5.505	369	31.3	0.0150966	9	0.350478	1.1	1	5.505	374.8	9.4	0.0210377
13	5.555	380	32.2	0.0659832	3	0.537237	1.6	1.5	5.555	383.1	2.8	0.107081
14	5.6	390	33.1	0.396356	6	3.94742	11.9	11	5.6	389	5.9	0.377295
15	5.673	406	34.4	0.283622	8	3.88272	11.7	10.8	5.673	403.9	7.5	0.290241
16	5.755	424	36	0.0742989	8	1.05827	3.2	3	5.755	422.9	8	0.0745158
17	5.845	444	37.7	0.00750421	1	0.00984311	0	0	5.845	444.3	1	0.00552897
18	5.925	462	39.2	0.0147537	3	0.0798941	0.2	0.2	5.925	462	3.1	0.014567
19	5.964	471	39.9	0.0342692	3	0.19387	0.6	0.5	5.964	471	3.4	0.0320541
20	5.995	478	40.5	0.0221658	6	0.310464	0.9	0.9	5.995	480.3	6.4	0.0275201
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.039	488	41.4	0.262825	3	1.41271	4.3	3.9	6.039	487.7	3.2	0.249672
22	6.14	511	43.3	0.283413	4	1.8417	5.6	5.1	6.14	510.7	3.7	0.282247
23	6.267	540	45.8	0.0374995	6	0.32711	1	0.9	6.267	540.3	6.3	0.0293761
24	6.363	562	47.7	0.0533235	16	1.43961	4.3	4	6.363	563.8	16.1	0.0504771
25	6.464	585	49.6	0.0336831	11	0.510432	1.5	1.4	6.464	583.2	10.7	0.0268871
26	7.131	695	58.9	0.0299181	8	0.385424	1.2	1.1	7.131	697.2	8.3	0.0261687
27	7.317	729	61.8	0.0460139	18	1.29323	3.9	3.6	7.317	731	18	0.0405356

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	83	7	0.300114	12	5.69382	24.2	18.4	3.5	84.3	11.8	0.273286
2	4.55	228	19.3	0.285799	5	2.3564	10	7.6	4.55	227.4	4.8	0.275765
3	5.2	304	25.8	0.367772	5	2.9727	12.6	9.6	5.2	304	4.6	0.367571
4	5.85	450	38.2	0.394339	3	2.28139	9.7	7.4	5.85	450	3.3	0.387927
5	6.55	611	51.8	0.354021	4	2.33827	9.9	7.6	6.55	610.9	4	0.330434
6	6.85	650	55.1	0.143222	5	1.0525	4.5	3.4	6.85	650.1	4.6	0.130039
7	7.35	742	62.9	0.177769	6	1.64688	7	5.3	7.35	740.8	5.6	0.164648
8	8.15	908	77	0.0447155	9	0.698033	3	2.3	8.15	908.7	9.5	0.0416562
9	8.45	968	82.1	0.0704442	7	0.843544	3.6	2.7	8.45	968.5	7	0.0675365
10	8.65	1008	85.5	0.0463293	5	0.43169	1.8	1.4	8.65	1007.4	5.4	0.0448079
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1139	96.6	0.350139	6	3.23016	13.7	10.4	9.3	1137.7	5.8	0.313394

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 157

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 30.800 4.550 7.100 5.200 9.800 5.850 7.300 6.550 8.800 6.850 2.300 7.350 7.200 8.150 3.200 8.450 2.900 8.650 2.000 9.300 18.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 3 PeakCount: 11 Return to Lane Navigator
3.500 11.600 3.704 3.300 4.303 7.000 4.487 1.800 4.550 3.100 4.658 0.400 4.825 2.200 4.942 0 5.092 2.700 5.200 4.400 5.268 6.400 5.321 4.800 5.423 1.300 5.535 0.100 5.583 0 5.632 6.400 5.724 4.500 5.850 1.800 5.954 2.200 6.062 2.100 6.102 0 6.142 1.100 6.174 1.900 6.222 0.500 6.306 2.500 6.414 0.300 6.550 2.700 6.850 0.500 7.350 5.400 7.456 1.400 8.150 4.400 8.450 1.900 8.650 1.200 9.300 10.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 4 PeakCount: 34 Return to Lane Navigator
3.807 3.400 4.114 21.200 4.284 12.500 4.434 0.900 4.575 0.800 4.745 2.200 4.915 0.400 5.017 1.100 5.085 1.000 5.179 2.300 5.252 9.600 5.306 6.100 5.424 1.100 5.527 0.100 5.655 7.600 5.754 1.500 5.803 3.400 5.931 1.400 5.964 7.600 6.043 1.900 6.121 1.200 6.159 2.500 6.196 0.200 6.262 1.800 6.420 0.300 7.250 4.300 7.436 3.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 5 PeakCount: 27 Return to Lane Navigator
3.500 30.700 4.550 8.600 5.200 10.600 5.850 8.600 6.550 10.500 6.850 1.900 7.350 7.500 8.150 2.800 8.450 2.600 8.650 2.000 9.300 14.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 6 PeakCount: 11 Return to Lane Navigator
3.500 8.100 3.850 2.600 4.142 10.100 4.317 5.700 4.470 0.300 4.550 2.500 4.660 0 4.744 0.100 4.837 0.700 4.921 1.600 5.014 1.400 5.090 0.600 5.200 2.400 5.246 5.800 5.316 2.900 5.414 0.900 5.511 0.200 5.548 0 5.604 7.300 5.678 6.500 5.771 2.000 5.850 2.300 5.965 0.400 6.018 0.200 6.054 2.800 6.169 3.300 6.297 1.000 6.417 3.000 6.550 2.900 6.850 1.200 7.350 5.800 7.463 1.400 8.150 4.200 8.450 1.200 8.650 0.900 9.300 7.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 7 PeakCount: 36 Return to Lane Navigator
3.747 3.300 4.103 16.900 4.307 9.400 4.466 0.600 4.631 0.400 4.869 1.100 4.988 0.100 5.064 0.300 5.264 10.700 5.327 3.500 5.409 0.700 5.505 1.100 5.555 1.600 5.600 11.900 5.673 11.700 5.755 3.200 5.845 0 5.925 0.200 5.964 0.600 5.995 0.900 6.039 4.300 6.140 5.600 6.267 1.000 6.363 4.300 6.464 1.500 7.131 1.200 7.317 3.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 8 PeakCount: 27 Return to Lane Navigator
3.500 24.200 4.550 10.000 5.200 12.600 5.850 9.700 6.550 9.900 6.850 4.500 7.350 7.000 8.150 3.000 8.450 3.600 8.650 1.800 9.300 13.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 9 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pacific Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.