RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Kawakawa ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Euthynnus affinis
FDA Seafood List Common Name: Kawakawa
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970129gel01
Unique Web Code: kwh01Srr
Tissue Aliquot Code: euthaffi A97-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	112	13.1	0.0860654	7	1.02881	6	5.2	4.55	111.8	7	0.0835126
2	5.2	202	23.6	0.126667	6	1.28657	7.5	6.5	5.2	202.1	5.7	0.126263
3	5.85	299	34.9	0.107701	6	1.17002	6.9	5.9	5.85	299.3	6.2	0.10636
4	6.55	429	50.1	0.106892	12	2.2525	13.2	11.4	6.55	426.7	12.4	0.102228
5	6.85	464	54.1	0.0878926	8	1.19235	7	6	6.85	464.3	7.6	0.0880326
6	7.35	532	62.1	0.146086	9	2.19219	12.9	11.1	7.35	531.8	8.6	0.143328
7	8.15	657	76.7	0.119202	8	1.6838	9.9	8.5	8.15	656.5	8.3	0.114687
8	8.45	722	84.2	0.144302	9	2.24865	13.2	11.4	8.45	720.9	9	0.141377
9	8.65	778	90.8	0.0878802	6	0.866299	5.1	4.4	8.65	778	5.6	0.0873628
10	9.3	843	98.4	0.16256	12	3.13001	18.4	15.8	9.3	840.6	11.8	0.149641

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.877	23	2.7	0.127583	9	2.11117	4.8	4.4	3.877	23.1	9.4	0.126609
2	5.037	172	20	0.399733	8	6.1265	13.9	12.9	5.037	172.5	8.4	0.412804
3	5.837	293	34.1	0.136637	6	1.55377	3.5	3.3	5.837	292.8	6.4	0.135921
4	6.521	422	49.2	0.174177	14	4.15686	9.5	8.7	6.521	419.2	14.4	0.162867
5	6.63	437	50.9	0.561057	4	4.32851	9.8	9.1	6.63	436.3	4.5	0.54715
6	6.774	454	52.9	0.237398	5	2.10773	4.8	4.4	6.774	455.2	5.3	0.226243
7	6.97	479	55.8	0.095754	15	3.12365	7.1	6.6	6.97	486.1	14.7	0.119532
8	7.113	498	58	0.451628	6	4.25078	9.7	8.9	7.113	497.6	6	0.399216
9	7.241	515	60	0.0443656	4	0.16082	0.4	0.3	7.241	518	3.9	0.0234677
10	7.346	529	61.7	0.0298055	3	0.15429	0.4	0.3	7.346	530.5	3.3	0.0264263
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.486	551	64.2	0.172013	6	1.72257	3.9	3.6	7.486	551.5	5.7	0.170124
12	7.612	571	66.6	0.34392	7	3.89971	8.9	8.2	7.612	570.9	6.6	0.335738
13	7.714	587	68.4	0.109357	8	1.47322	3.4	3.1	7.714	587.6	7.9	0.105436
14	7.948	624	72.7	0.104824	6	0.967361	2.2	2	7.948	624.2	6	0.0905597
15	7.998	632	73.7	0.114573	8	1.41359	3.2	3	7.998	637.4	8.5	0.0943772
16	8.336	695	81	0.0550797	9	0.957532	2.2	2	8.336	698.3	9.3	0.0578403
17	8.402	709	82.6	0.220336	5	1.72827	3.9	3.6	8.402	709.2	4.6	0.211912
18	8.553	748	87.2	0.122683	9	1.76948	4	3.7	8.553	745.6	9.5	0.10534
19	8.685	779	90.8	0.0421669	9	0.656034	1.5	1.4	8.685	780.2	9.3	0.0396986
20	8.955	806	93.9	0.0244685	5	0.247858	0.6	0.5	8.955	807.1	5.3	0.0262053
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.235	834	97.2	0.095672	6	1.06542	2.4	2.2	9.235	834.4	6.1	0.0986063

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	107	12.5	0.055324	7	0.628744	4.9	4.2	4.55	108	6.7	0.0527362
2	5.2	184	21.4	0.103461	6	0.995269	7.7	6.6	5.2	183.8	5.5	0.10121
3	5.85	291	33.9	0.0996388	7	1.21487	9.4	8	5.85	290.4	7	0.0975303
4	6.55	426	49.6	0.108682	11	1.96493	15.3	13	6.55	424.8	11.1	0.0998672
5	6.85	462	53.8	0.0714977	8	1.00153	7.8	6.6	6.85	462.5	8.2	0.0688386
6	7.35	527	61.4	0.0872707	9	1.29256	10	8.5	7.35	526.1	8.6	0.0847695
7	8.15	655	76.3	0.09755	8	1.37514	10.7	9.1	8.15	655	8.4	0.0927414
8	8.45	716	83.4	0.106249	9	1.64159	12.8	10.8	8.45	714.9	8.9	0.103536
9	8.65	773	90	0.0724253	6	0.722185	5.6	4.8	8.65	772.3	5.7	0.071159
10	9.3	838	97.6	0.106552	12	2.03537	15.8	13.4	9.3	837.8	11.7	0.0978215

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.952	34	3.9	0.0472892	10	0.810492	2.1	2	3.952	31.9	9.6	0.0477076
2	4.55	114	13.2	0.0344346	5	0.32842	0.8	0.8	4.55	113.5	5.5	0.0337713
3	4.998	174	20.2	0.301608	7	3.83774	9.7	9.3	4.998	173.8	7.1	0.303762
4	5.2	201	23.3	0.0577235	5	0.497368	1.3	1.2	5.2	201.7	4.8	0.0584086
5	5.85	299	34.7	0.0833564	8	1.1729	3	2.9	5.85	300.8	7.8	0.0845806
6	6.528	423	49.1	0.140106	15	3.952	10	9.6	6.528	425.4	14.7	0.151232
7	6.576	430	49.9	0.175703	3	0.179375	0.5	0.4	6.576	430.2	2.9	0.0348948
8	6.674	441	51.2	0.585158	5	4.73978	12	11.5	6.674	441.3	4.8	0.55179
9	6.841	460	53.4	0.315221	7	3.55286	9	8.6	6.841	460.8	7.1	0.283707
10	7.007	483	56	0.148118	14	3.866	9.8	9.4	7.007	483.8	14	0.155558
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.129	500	58	0.483694	6	4.62775	11.7	11.2	7.129	499.8	5.9	0.439964
12	7.35	531	61.6	0.145345	8	1.85038	4.7	4.5	7.35	530	8.2	0.127923
13	7.5	555	64.4	0.113149	6	1.24321	3.2	3	7.5	556.4	6.4	0.109663
14	7.619	574	66.6	0.277223	6	2.99485	7.6	7.3	7.619	573.1	6.2	0.272842
15	7.981	632	73.3	0.0794184	5	0.47369	1.2	1.2	7.981	631.9	4.5	0.059309
16	8.15	659	76.5	0.0379939	8	0.537757	1.4	1.3	8.15	657.9	8	0.0379715
17	8.359	705	81.8	0.0989226	6	1.07836	2.7	2.6	8.359	704.5	6.1	0.100032
18	8.45	725	84.1	0.0270521	6	0.266954	0.7	0.6	8.45	725.2	6.1	0.0244965
19	8.55	752	87.2	0.0380472	11	0.666825	1.7	1.6	8.55	756.1	10.8	0.0348391
20	8.65	779	90.4	0.0809804	10	1.27353	3.2	3.1	8.65	778.1	9.9	0.0724356
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.971	809	93.9	0.0199808	10	0.397043	1	1	8.971	803.2	10.5	0.0214277
22	9.239	834	96.8	0.0870487	7	1.11573	2.8	2.7	9.239	833.6	7.5	0.0842291

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.927	29	3.4	0.111962	10	1.90894	4.4	4.1	3.927	28.3	9.6	0.111821
2	4.981	166	19.2	0.389181	8	5.81952	13.6	12.5	4.981	166.4	8.2	0.398246
3	5.847	295	34.2	0.11821	6	1.2729	3	2.7	5.847	295.1	6.2	0.116207
4	6.486	414	48	0.122031	7	1.48088	3.4	3.2	6.486	413.4	7.5	0.111828
5	6.55	426	49.4	0.151989	5	1.2196	2.8	2.6	6.55	425.9	4.6	0.148615
6	6.672	439	50.9	0.520003	5	4.68771	10.9	10.1	6.672	438.9	4.9	0.540674
7	6.857	459	53.2	0.247991	6	2.42474	5.6	5.2	6.857	458.9	5.7	0.239033
8	7.02	482	55.9	0.118987	15	3.18695	7.4	6.8	7.02	484.9	14.8	0.12189
9	7.134	498	57.7	0.445748	6	4.56664	10.6	9.8	7.134	498	6.3	0.407486
10	7.311	523	60.6	0.0660554	6	0.666641	1.6	1.4	7.311	522.3	6	0.0628921
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.378	533	61.8	0.053166	4	0.393533	0.9	0.8	7.378	532.8	4.4	0.0500796
12	7.516	555	64.3	0.18609	6	1.78947	4.2	3.8	7.516	555.8	5.7	0.178656
13	7.648	576	66.7	0.325819	8	4.20046	9.8	9	7.648	576.2	7.6	0.309861
14	7.949	624	72.3	0.015572	4	0.100463	0.2	0.2	7.949	623.6	3.6	0.0156281
15	8.006	633	73.3	0.0212974	5	0.166299	0.4	0.4	8.006	635.6	4.9	0.0191638
16	8.384	707	81.9	0.222374	7	2.59373	6	5.6	8.384	705.8	6.9	0.211806
17	8.551	749	86.8	0.114974	22	4.15909	9.7	8.9	8.551	747.7	21.8	0.107456
18	8.66	777	90	0.0987955	11	0.996619	2.3	2.1	8.66	780.6	10.5	0.0533285
19	9.235	833	96.5	0.113993	7	1.29324	3	2.8	9.235	832.8	6.5	0.112129

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	106	12.3	0.110086	9	1.67695	8.4	6.9	4.55	107.2	8.9	0.106005
2	5.2	188	21.8	0.142267	6	1.48583	7.5	6.1	5.2	188.5	5.9	0.142091
3	5.85	292	33.8	0.119179	7	1.39893	7	5.7	5.85	291.5	6.7	0.117319
4	6.55	425	49.2	0.128763	10	2.34875	11.8	9.6	6.55	422.8	10.3	0.12903
5	6.85	455	52.7	0.116329	7	1.50798	7.6	6.2	6.85	455.6	7.3	0.116823
6	7.35	526	60.9	0.175482	9	2.74405	13.8	11.2	7.35	525.7	8.8	0.176011
7	8.15	653	75.6	0.159776	9	2.29426	11.5	9.4	8.15	653	8.6	0.151223
8	8.45	718	83.1	0.198771	8	2.87874	14.4	11.8	8.45	716.8	8.5	0.191238
9	8.65	773	89.5	0.10979	6	1.06727	5.4	4.4	8.65	772.7	5.7	0.105813
10	9.3	839	97.1	0.163296	9	2.53165	12.7	10.3	9.3	837.4	9.2	0.154461

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 92

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.000 5.200 7.500 5.850 6.900 6.550 13.200 6.850 7.000 7.350 12.900 8.150 9.900 8.450 13.200 8.650 5.100 9.300 18.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
3.877 4.800 5.037 13.900 5.837 3.500 6.521 9.500 6.630 9.800 6.774 4.800 6.970 7.100 7.113 9.700 7.241 0.400 7.346 0.400 7.486 3.900 7.612 8.900 7.714 3.400 7.948 2.200 7.998 3.200 8.336 2.200 8.402 3.900 8.553 4.000 8.685 1.500 8.955 0.600 9.235 2.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 21 Return to Lane Navigator
4.550 4.900 5.200 7.700 5.850 9.400 6.550 15.300 6.850 7.800 7.350 10.000 8.150 10.700 8.450 12.800 8.650 5.600 9.300 15.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
3.952 2.100 4.550 0.800 4.998 9.700 5.200 1.300 5.850 3.000 6.528 10.000 6.576 0.500 6.674 12.000 6.841 9.000 7.007 9.800 7.129 11.700 7.350 4.700 7.500 3.200 7.619 7.600 7.981 1.200 8.150 1.400 8.359 2.700 8.450 0.700 8.550 1.700 8.650 3.200 8.971 1.000 9.239 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
3.927 4.400 4.981 13.600 5.847 3.000 6.486 3.400 6.550 2.800 6.672 10.900 6.857 5.600 7.020 7.400 7.134 10.600 7.311 1.600 7.378 0.900 7.516 4.200 7.648 9.800 7.949 0.200 8.006 0.400 8.384 6.000 8.551 9.700 8.660 2.300 9.235 3.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 19 Return to Lane Navigator
4.550 8.400 5.200 7.500 5.850 7.000 6.550 11.800 6.850 7.600 7.350 13.800 8.150 11.500 8.450 14.400 8.650 5.400 9.300 12.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Kawakawa ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Kawakawa ---- Go to Display Gel Lane Navigator.