RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Chinook Salmon ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Oncorhynchus tshawytsha
FDA Seafood List Common Name: Chinook Salmon
FDA Seafood List Market Name: Salmon, Chinook (King, Spring)
Unique IEF Gel Code: 19960306gel01
Unique Web Code: kgg01Srr
Tissue Aliquot Code: oncotsha I94-001 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	26	2.3	0.278195	11	4.91369	12.2	8.9	3.5	30.1	10.7	0.259703
2	4.55	183	16.4	0.263474	7	3.05673	7.6	5.5	4.55	181.7	6.7	0.258322
3	5.2	266	23.8	0.237067	8	3.14945	7.8	5.7	5.2	264.3	7.6	0.23405
4	5.85	419	37.5	0.595533	4	4.27898	10.6	7.7	5.85	418.6	4	0.604924
5	6.55	588	52.6	0.274728	5	2.05115	5.1	3.7	6.55	587.2	4.5	0.257047
6	6.85	629	56.3	0.167705	8	2.02929	5	3.7	6.85	629.8	7.8	0.146313
7	7.35	725	64.9	0.608359	7	7.26717	18	13.1	7.35	723.7	7.2	0.569734
8	8.15	887	79.4	0.16601	7	2.14565	5.3	3.9	8.15	886.1	7.3	0.165449
9	8.45	942	84.3	0.295197	5	2.66774	6.6	4.8	8.45	942.3	5.3	0.285849
10	8.65	979	87.6	0.237558	6	2.24081	5.6	4	8.65	979.1	5.5	0.227971
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1099	98.4	0.59238	7	6.47092	16.1	11.7	9.3	1098	6.7	0.548547

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.523	28	2.5	0.470863	3	2.88959	4.3	3.6	3.523	28.1	3.5	0.467259
2	4.667	197	17.6	0.260393	5	2.05074	3	2.5	4.667	197.2	4.5	0.255098
3	4.861	222	19.8	0.167449	6	1.94211	2.9	2.4	4.861	219.7	6.3	0.174816
4	5.364	304	27.2	0.78993	6	8.04578	11.9	9.9	5.364	303.7	5.6	0.811504
5	5.556	349	31.2	0.325804	6	3.46703	5.1	4.3	5.556	350	5.9	0.33277
6	5.599	359	32.1	0.304029	2	1.15967	1.7	1.4	5.599	359.2	2.4	0.277888
7	5.65	371	33.2	0.141475	3	0.873902	1.3	1.1	5.65	370.6	3.5	0.142563
8	5.701	383	34.2	0.0838992	4	0.701723	1	0.9	5.701	383.8	4.4	0.0896204
9	5.882	426	38.1	0.534014	4	3.35784	5	4.1	5.882	426.6	3.5	0.540991
10	5.994	454	40.6	0.268135	4	1.69631	2.5	2.1	5.994	454.1	3.7	0.259993
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.191	503	45	0.650383	3	3.87637	5.7	4.8	6.191	503.3	3.4	0.650154
12	6.303	531	47.5	0.304454	3	1.58631	2.4	2	6.303	531.1	3	0.302617
13	6.339	540	48.3	0.124978	3	0.706042	1	0.9	6.339	540.5	3.1	0.129996
14	6.508	582	52	0.868979	4	5.99772	8.9	7.4	6.508	582.5	3.8	0.878988
15	6.659	607	54.2	0.348519	7	4.01813	6	5	6.659	605.2	6.9	0.328886
16	6.771	622	55.6	0.168398	2	0.747307	1.1	0.9	6.771	621.7	2.4	0.172118
17	6.839	631	56.4	0.218181	3	1.09852	1.6	1.4	6.839	630.6	2.8	0.218793
18	6.911	644	57.6	0.111819	3	0.519338	0.8	0.6	6.911	644.6	2.6	0.113755
19	7.112	682	60.9	0.41491	2	1.73065	2.6	2.1	7.112	681.6	2.3	0.427908
20	7.186	696	62.2	0.0519444	4	0.407181	0.6	0.5	7.186	694.2	3.7	0.0617254
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.239	706	63.1	0.349225	2	1.35202	2	1.7	7.239	705.7	2.2	0.353419
22	7.37	731	65.3	0.145134	2	0.633385	0.9	0.8	7.37	731.5	2.4	0.147598
23	7.445	746	66.7	0.707369	3	3.27344	4.9	4	7.445	746.2	2.6	0.715575
24	7.769	811	72.5	0.626772	4	4.49024	6.7	5.5	7.769	811.2	3.9	0.642365
25	8.008	859	76.8	0.349488	4	2.37327	3.5	2.9	8.008	858.7	4	0.338175
26	8.647	981	87.7	0.322658	11	5.87458	8.7	7.3	8.647	981.3	10.5	0.314988
27	8.874	1023	91.4	0.18199	8	2.58375	3.8	3.2	8.874	1023.2	8	0.183055

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	18	1.6	0.238487	9	3.46424	9.2	6.9	3.5	21.5	9.3	0.209196
2	4.55	176	15.8	0.377823	5	3.36735	8.9	6.7	4.55	175.9	5.2	0.366446
3	5.2	260	23.3	0.37034	5	2.98491	7.9	5.9	5.2	260.1	4.6	0.366049
4	5.85	412	37	0.745479	4	4.84239	12.8	9.6	5.85	411.5	3.6	0.764172
5	6.55	592	53.1	0.327081	4	2.14837	5.7	4.3	6.55	591.6	3.8	0.320704
6	6.85	631	56.6	0.163848	8	1.99091	5.3	3.9	6.85	630.6	8.3	0.135027
7	7.35	724	64.9	0.641269	6	6.90394	18.3	13.7	7.35	723.2	6.4	0.610398
8	8.15	883	79.2	0.156894	8	2.07566	5.5	4.1	8.15	882.6	7.6	0.154168
9	8.45	941	84.4	0.291158	6	2.90789	7.7	5.8	8.45	941.7	5.8	0.281124
10	8.65	979	87.8	0.248357	5	2.09259	5.5	4.2	8.65	978.8	4.8	0.245069
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1100	98.7	0.511894	6	4.99351	13.2	9.9	9.3	1098.5	5.9	0.478326

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 19
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 21
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	25	2.2	0.450303	7	5.04659	5.2	4.5	3.5	25.3	6.7	0.422627
2	4.55	173	15.5	0.239373	4	0.999146	1	0.9	4.55	169.6	3.5	0.15936
3	4.608	180	16.1	0.174838	6	2.5668	2.7	2.3	4.608	176.5	5.8	0.247908
4	4.758	198	17.7	0.145671	6	1.67667	1.7	1.5	4.758	198.4	6.3	0.149137
5	5.2	251	22.5	0.268534	8	3.47382	3.6	3.1	5.2	250.1	8.2	0.237798
6	5.328	281	25.2	0.706175	6	7.92641	8.2	7.1	5.328	281.1	6.4	0.701266
7	5.525	327	29.3	0.23856	6	2.86486	3	2.5	5.525	327.6	6.4	0.250893
8	5.568	337	30.2	0.293588	3	1.18407	1.2	1.1	5.568	337	2.6	0.257218
9	5.623	350	31.3	0.182771	3	1.01622	1.1	0.9	5.623	349.9	3.1	0.182824
10	5.85	403	36.1	0.464783	3	2.49268	2.6	2.2	5.85	403.3	3.3	0.427797
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.872	409	36.6	0.630408	4	4.40818	4.6	3.9	5.872	409.7	4	0.625827
12	5.992	441	39.5	0.25498	4	1.65256	1.7	1.5	5.992	441.1	3.8	0.246008
13	6.202	497	44.5	0.684434	3	3.93695	4.1	3.5	6.202	497	3.2	0.685516
14	6.314	527	47.2	0.30902	3	1.52746	1.6	1.4	6.314	526.6	2.9	0.29956
15	6.348	536	48	0.150185	3	0.842218	0.9	0.7	6.348	536.1	3	0.157951
16	6.516	581	52	0.812107	3	4.99702	5.2	4.4	6.516	580.1	3.4	0.834276
17	6.55	590	52.8	0.201934	6	2.00495	2.1	1.8	6.55	589.6	5.8	0.194647
18	6.662	605	54.2	0.278198	6	2.65188	2.7	2.4	6.662	604.3	5.6	0.26881
19	6.79	622	55.7	0.164948	2	0.657691	0.7	0.6	6.79	622.1	2.3	0.157942
20	6.85	630	56.4	0.236773	4	1.52813	1.6	1.4	6.85	630.3	3.8	0.224712
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.937	646	57.8	0.112671	4	0.65895	0.7	0.6	6.937	645.2	3.8	0.0974993
22	7.138	683	61.1	0.338435	2	1.38101	1.4	1.2	7.138	683.2	2.3	0.340538
23	7.274	708	63.4	0.272074	3	1.30494	1.4	1.2	7.274	708.1	2.7	0.268014
24	7.35	722	64.6	0.553822	5	5.1043	5.3	4.5	7.35	722	5.2	0.549459
25	7.412	734	65.7	0.124644	2	0.508405	0.5	0.5	7.412	733.9	2.3	0.125809
26	7.485	748	67	0.609858	3	3.32187	3.4	3	7.485	747.9	3.1	0.610555
27	7.812	811	72.6	0.557404	5	4.67454	4.8	4.2	7.812	810.6	4.7	0.564957
28	8.072	861	77.1	0.291133	5	2.57574	2.7	2.3	8.072	861.3	5.5	0.264598
29	8.15	876	78.4	0.197679	8	2.87903	3	2.6	8.15	876.4	8.1	0.201065
30	8.45	944	84.5	0.346008	9	4.87034	5	4.3	8.45	944.6	9.3	0.29646
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	981	87.8	0.421408	11	7.35991	7.6	6.5	8.65	979.1	10.8	0.382815
32	8.867	1021	91.4	0.177034	8	2.43677	2.5	2.2	8.867	1021.1	7.8	0.175522
33	9.3	1101	98.6	0.58158	6	6.00543	6.2	5.3	9.3	1099.9	6.2	0.544446

PeakCounter: 33

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 20
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 22
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.528	26	2.3	0.339092	4	2.69186	3.3	2.8	3.528	26.1	4.4	0.343557
2	4.672	186	16.7	0.263979	5	2.33535	2.9	2.5	4.672	186	5.1	0.259595
3	4.867	210	18.8	0.185816	6	2.1463	2.6	2.3	4.867	208.6	6.2	0.195095
4	5.385	295	26.4	0.770873	5	7.68265	9.5	8.1	5.385	295	5.4	0.80431
5	5.577	341	30.6	0.283208	7	3.55642	4.4	3.8	5.577	341.3	7.1	0.281329
6	5.615	350	31.4	0.343432	2	1.01266	1.2	1.1	5.615	350.5	1.9	0.29999
7	5.691	368	33	0.13006	4	0.885539	1.1	0.9	5.691	368.8	4.4	0.114418
8	5.921	425	38.1	0.460511	4	3.00715	3.7	3.2	5.921	424.7	3.7	0.456569
9	6.026	453	40.6	0.261905	4	1.69011	2.1	1.8	6.026	453.1	3.8	0.251228
10	6.213	503	45.1	0.686301	3	3.66032	4.5	3.9	6.213	503.3	2.9	0.702066
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.318	531	47.6	0.352691	3	1.72338	2.1	1.8	6.318	531.3	2.7	0.354036
12	6.355	541	48.5	0.161821	4	1.14964	1.4	1.2	6.355	540.9	4	0.164076
13	6.501	580	52	0.905899	4	6.10924	7.5	6.5	6.501	580.9	3.7	0.942995
14	6.64	605	54.2	0.444791	8	5.32891	6.6	5.6	6.64	603.5	7.6	0.396199
15	6.753	620	55.6	0.280994	2	1.14746	1.4	1.2	6.753	620.2	2.4	0.275315
16	6.812	628	56.3	0.303839	3	1.45139	1.8	1.5	6.812	628.3	2.7	0.300459
17	6.903	643	57.6	0.21868	2	0.848919	1	0.9	6.903	642.8	2.2	0.216162
18	7.101	680	60.9	0.539971	2	2.14185	2.6	2.3	7.101	679.7	2.2	0.537319
19	7.149	689	61.7	0.118293	3	0.698318	0.9	0.7	7.149	689.2	3.4	0.116252
20	7.224	703	63	0.460684	2	1.81168	2.2	1.9	7.224	703	2.2	0.460252
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.358	728	65.2	0.16915	2	0.685394	0.8	0.7	7.358	728.3	2.3	0.168976
22	7.415	739	66.2	0.528878	3	3.33839	4.1	3.5	7.415	738.4	3.5	0.539207
23	7.731	800	71.7	0.507855	6	5.17733	6.4	5.5	7.731	799.8	5.8	0.504937
24	7.984	849	76.1	0.395213	4	2.65219	3.3	2.8	7.984	849	3.9	0.381771
25	8.677	985	88.3	0.42498	14	9.44511	11.6	10	8.677	983.3	13.5	0.394362
26	8.877	1022	91.6	0.353258	14	8.51607	10.5	9	8.877	1019.2	14.3	0.335784
27	9.373	1114	99.8	0.166956	1	0.38236	0.5	0.4	9.373	1114	1	0.215701

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 21
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 23
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	20	1.8	0.249708	8	3.22407	8.1	5.9	3.5	21.6	7.8	0.232326
2	4.55	170	15.2	0.244039	7	3.07762	7.7	5.7	4.55	170.7	6.9	0.251534
3	5.2	252	22.6	0.229104	7	2.72103	6.9	5	5.2	252.2	6.6	0.232981
4	5.85	410	36.7	0.561599	5	4.60268	11.6	8.5	5.85	409.8	4.6	0.568858
5	6.55	597	53.5	0.314456	4	2.24731	5.7	4.1	6.55	596.7	4.1	0.309091
6	6.85	637	57.1	0.173705	8	2.0378	5.1	3.8	6.85	637.1	7.9	0.145633
7	7.35	732	65.6	0.653462	7	7.88012	19.8	14.5	7.35	730.9	7.2	0.617021
8	8.15	887	79.5	0.184444	7	2.33852	5.9	4.3	8.15	887.4	7.4	0.179314
9	8.45	945	84.7	0.300947	5	2.87307	7.2	5.3	8.45	944.4	5.5	0.296257
10	8.65	980	87.8	0.248815	5	2.16221	5.4	4	8.65	980.3	4.9	0.249592
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1101	98.7	0.636637	6	6.55263	16.5	12.1	9.3	1099.6	6.4	0.5788

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 120

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 12.200 4.550 7.600 5.200 7.800 5.850 10.600 6.550 5.100 6.850 5.000 7.350 18.000 8.150 5.300 8.450 6.600 8.650 5.600 9.300 16.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
3.523 4.300 4.667 3.000 4.861 2.900 5.364 11.900 5.556 5.100 5.599 1.700 5.650 1.300 5.701 1.000 5.882 5.000 5.994 2.500 6.191 5.700 6.303 2.400 6.339 1.000 6.508 8.900 6.659 6.000 6.771 1.100 6.839 1.600 6.911 0.800 7.112 2.600 7.186 0.600 7.239 2.000 7.370 0.900 7.445 4.900 7.769 6.700 8.008 3.500 8.647 8.700 8.874 3.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 19 PeakCount: 27 Return to Lane Navigator
3.500 9.200 4.550 8.900 5.200 7.900 5.850 12.800 6.550 5.700 6.850 5.300 7.350 18.300 8.150 5.500 8.450 7.700 8.650 5.500 9.300 13.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 20 PeakCount: 11 Return to Lane Navigator
3.500 5.200 4.550 1.000 4.608 2.700 4.758 1.700 5.200 3.600 5.328 8.200 5.525 3.000 5.568 1.200 5.623 1.100 5.850 2.600 5.872 4.600 5.992 1.700 6.202 4.100 6.314 1.600 6.348 0.900 6.516 5.200 6.550 2.100 6.662 2.700 6.790 0.700 6.850 1.600 6.937 0.700 7.138 1.400 7.274 1.400 7.350 5.300 7.412 0.500 7.485 3.400 7.812 4.800 8.072 2.700 8.150 3.000 8.450 5.000 8.650 7.600 8.867 2.500 9.300 6.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 19 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 21 PeakCount: 33 Return to Lane Navigator
3.528 3.300 4.672 2.900 4.867 2.600 5.385 9.500 5.577 4.400 5.615 1.200 5.691 1.100 5.921 3.700 6.026 2.100 6.213 4.500 6.318 2.100 6.355 1.400 6.501 7.500 6.640 6.600 6.753 1.400 6.812 1.800 6.903 1.000 7.101 2.600 7.149 0.900 7.224 2.200 7.358 0.800 7.415 4.100 7.731 6.400 7.984 3.300 8.677 11.600 8.877 10.500 9.373 0.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 20 LANE TYPE: Specimen ACTUAL GEL LANE: 22 PeakCount: 27 Return to Lane Navigator
3.500 8.100 4.550 7.700 5.200 6.900 5.850 11.600 6.550 5.700 6.850 5.100 7.350 19.800 8.150 5.900 8.450 7.200 8.650 5.400 9.300 16.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 21 LANE TYPE: pI Markers ACTUAL GEL LANE: 23 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Chinook Salmon ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Chinook Salmon ---- Go to Display Gel Lane Navigator.