Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Pleuronectes vetulus
FDA Seafood List Common Name: English Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19930817gel04
Unique Web Code: esh03Srr
Tissue Aliquot Code: pleuvetu 002 #1 Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Co-Migrated
Lane c
Specimen
Lane d
pI Markers
Lane e
Co-Migrated
Lane f
Specimen
Lane g
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 1
LANE TYPE: pI Markers
ACTUAL GEL LANE: 1
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5512914.60.0031218580.03943463.92.14.55128.68.40.00263875
25.2221250.0033016970.0313623.11.65.2219.36.80.00258353
35.8533337.60.0044130270.04902014.82.65.85336.37.10.00387785
46.5546152.10.00813814100.1335751376.55457.99.70.00777437
57.3556463.70.00326736130.03322713.21.77.35567.813.10.00142992
68.1567776.50.012041150.10275105.48.156765.30.0110323
78.4574183.70.0093266160.08894318.74.68.45741.35.80.0086047
88.6579589.80.0059694450.051192552.78.65795.24.90.00586835
99.384995.90.0205781140.49429148.325.79.3849.814.40.0193625
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 2
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.964404.50.01722660.160880.70.63.96440.96.20.0145511
24.40210411.80.09348230.2578231.20.94.402102.63.40.0431261
34.50411913.50.225214166.3168729.3224.50412416.30.218246
45.26123126.10.031339730.1615670.70.65.261231.52.90.0311079
55.33124327.50.055810130.2711351.30.95.331243.52.70.0569365
65.43126029.40.19613941.43276.655.431260.24.40.185202
75.51927531.10.21627841.412116.64.95.519274.63.80.211494
85.68930434.40.43711842.8152513.19.85.689304.33.70.432938
95.80132336.50.067700340.5034162.31.85.801322.94.20.0680016
105.88633838.20.067870450.5633542.625.886337.34.70.0673963
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.93534739.30.31422441.895818.86.65.935347.33.60.300215
126.16839044.10.0378073100.5218132.41.86.168394.210.40.0282236
136.3342047.50.11972281.757728.26.16.33417.58.50.117257
146.41143549.20.11219220.42964721.56.411435.82.40.0998962
156.53645851.80.02495250.245111.10.96.5364575.40.0255203
167.06952659.50.061073760.186520.90.77.069523.55.90.0177259
177.16553860.90.067514161.861838.66.57.165536.215.60.0672134
188.4575885.70.0190093140.42253221.58.45754.714.50.0164869
199.385096.20.0191016100.3334371.51.29.3850.69.60.0196532
PeakCounter: 19
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.0044450.022097950.1773980.80.64.00443.64.90.0203441
24.46811412.80.099689881.118315.23.94.468108.480.078397
34.58113114.80.264036135.412182518.84.581132.112.50.244008
45.25223326.20.035025230.1970690.90.75.252233.63.40.0328906
55.32924627.70.060808330.3226221.51.15.329245.93.10.0592261
65.43626429.70.19543941.364646.34.75.436264.44.20.185013
75.53128031.50.22440741.459536.75.15.531279.43.70.222288
85.70330934.80.43155742.6863712.49.35.7033093.50.42899
95.79832536.60.068422140.4692282.21.65.798324.93.90.0686954
105.86333637.80.059060140.4564342.11.65.863336.24.40.0590462
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.916346390.31813542.037689.47.15.916345.83.70.310101
126.13638743.60.0300029160.8118443.82.86.136395.216.30.0280406
136.296417470.11128981.485016.95.16.296415.77.90.105888
146.38843448.90.11203120.42972521.56.3884342.30.104556
157.08453059.70.0774629121.030274.83.67.084525.811.60.0500617
167.182542610.0863105162.1906410.17.67.18254515.80.0784122
PeakCounter: 16
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5512614.10.089658171.0018211.68.94.55125.96.70.0842105
25.222725.50.093572340.7226568.46.45.2226.84.40.0931642
35.8533537.60.057058580.7824789.175.85334.57.90.0555793
46.5546752.40.14886561.5685418.2146.55466.26.10.14578
57.3556363.20.036355460.3698764.33.37.35563.160.0349407
68.15686770.10729971.290291511.58.15685.46.80.107092
78.4574383.40.082411460.945074118.48.45742.96.50.0822067
88.6579288.90.068379850.6094987.15.48.65790.750.0689179
99.385195.50.11993761.3146415.311.79.3850.96.30.117078
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.026404.50.024597450.1967560.80.74.02640.44.90.0228118
24.3579110.20.01357650.09987480.40.34.35790.24.90.0115902
34.47410912.20.081748840.2700761.20.94.474106.53.60.0426537
44.58512614.20.247324135.6139424.118.64.58512713.30.237591
55.2823426.30.036195930.1975330.80.75.28233.730.0374392
65.35124627.60.057640230.3036011.315.351246.42.90.0584782
75.45826429.70.20357141.484786.44.95.458264.64.30.196868
85.54227831.20.23927441.691597.25.65.5422784.10.235069
95.70330534.30.47656143.0617513.110.25.703304.83.60.479155
105.792320360.072240840.47096221.65.792320.23.60.0728668
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.86233237.30.069301550.573252.51.95.862331.34.70.068881
125.90934138.30.34023342.10913975.909340.93.60.331846
136.12338242.90.0232321170.6859582.92.36.123392.816.60.0232943
146.29641546.60.12894691.8708886.26.296412.98.90.119064
156.38543248.50.13561320.5188522.21.76.385432.12.40.121441
166.48445150.70.019598340.1337740.60.46.484451.83.90.0191884
177.14253560.10.0723926111.191155.13.97.14253210.90.0615125
187.27555161.90.107173122.079788.96.97.275551.711.60.101439
198.4575184.40.0262635130.5795992.51.98.45753.213.30.0245947
209.384895.30.016285880.2021540.90.79.3846.38.20.0139094
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.0624550.019986360.1918110.90.74.06245.56.40.0167862
24.528116130.072881870.7261093.42.64.528109.87.10.0580369
34.65313515.10.260926135.7332926.820.34.653135.412.60.256493
45.31623826.70.01954730.1131650.50.45.316237.63.10.0204413
55.38124927.90.044998530.2446251.10.95.381249.330.0455551
65.48226629.80.18224441.350586.34.85.4822664.30.177996
75.55827931.20.2062941.521277.15.45.558278.44.20.204979
85.706304340.4107842.7119412.79.65.706304.63.70.412985
95.79531935.70.066641740.4407482.11.65.795319.63.60.0688234
105.86433137.10.062170250.504692.41.85.864330.54.80.0598619
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.9134038.10.31126642.090419.87.45.91340.240.297202
126.12138142.70.011788240.07242890.30.36.1213803.50.011526
136.28641346.20.11988991.898938.96.76.286410.99.30.114806
146.37343048.20.11876720.4443262.11.66.373430.52.30.106675
157.1153259.60.0718621121.2743464.57.1153011.70.0614223
167.22654661.10.111788112.072569.77.47.226548.411.10.105541
PeakCounter: 16
DISPLAY GEL LANE LETTER: Lane g
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5511512.90.070956160.74433297.14.55115.26.20.0671961
25.221323.90.069008950.5966947.25.75.2212.74.80.0699188
35.8532436.30.0463470.543226.65.25.85323.27.20.042404
46.5546251.70.12336271.4250517.213.66.55461.36.80.11791
57.3555962.60.033211250.3205063.93.17.35558.55.50.0331185
68.15679760.10285471.2334514.911.88.15679.26.70.103924
78.4573882.60.11242571.40691713.48.457387.10.111275
88.6579088.50.077949750.6486047.86.28.65789.44.80.0766417
99.384794.80.12111761.3595816.4139.3846.46.30.120904
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f g
MK CO SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.45 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 98
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 1
LANE TYPE: pI Markers
ACTUAL GEL LANE: 1
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 2
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 2
PeakCount: 19 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 16 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 16 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane g
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln