RFE Fish Species Identification Isoelectric Focusing Tools - Caribbean Red Snapper PAGPlate IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Caribbean Red Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus purpureus
FDA Seafood List Common Name: Caribbean Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940825gel01
Unique Web Code: csg01Srr
Tissue Aliquot Code: lutjpurp H94-002 #07p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.247036	12	4.90442	17.6	13.1	3.5	35.3	12.1	0.228768
2	4.55	179	15.8	0.130308	13	2.83989	10.2	7.6	4.55	183	13.1	0.122677
3	5.2	262	23.1	0.149716	13	3.3054	11.8	8.8	5.2	264.5	12.5	0.148948
4	5.85	418	36.8	0.310649	6	3.20581	11.5	8.6	5.85	418	5.7	0.317016
5	6.55	582	51.2	0.298879	6	2.92475	10.5	7.8	6.55	581.3	5.9	0.280595
6	6.85	623	54.8	0.095224	7	1.05795	3.8	2.8	6.85	623.2	7.3	0.0820829
7	7.35	716	63	0.273667	5	2.30799	8.3	6.2	7.35	714.9	4.8	0.268952
8	8.15	878	77.3	0.0989667	10	1.62543	5.8	4.3	8.15	876.8	9.6	0.0952057
9	8.45	938	82.6	0.150558	7	1.77975	6.4	4.8	8.45	937.7	6.9	0.146176
10	8.65	977	86	0.0846846	6	0.861408	3.1	2.3	8.65	976.9	6	0.0815226
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1098	96.7	0.219076	9	3.10544	11.1	8.3	9.3	1094.7	9	0.19501

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	38	3.3	0.182384	9	2.79798	5.1	4.5	3.5	38.8	9.4	0.168156
2	3.734	71	6.2	0.0513919	5	0.488254	0.9	0.8	3.734	70	5.5	0.0503822
3	4.131	127	11.2	0.0176183	5	0.15864	0.3	0.3	4.131	126.5	5.1	0.0174345
4	4.259	145	12.7	0.309665	5	2.5897	4.7	4.2	4.259	145.1	4.7	0.313074
5	4.429	169	14.8	0.0337636	7	0.386119	0.7	0.6	4.429	170.6	6.6	0.0329491
6	4.55	186	16.3	0.13399	6	1.47518	2.7	2.4	4.55	186.8	6.3	0.132108
7	4.672	201	17.6	0.0539658	6	0.608842	1.1	1	4.672	199.4	6.1	0.0564086
8	4.964	237	20.8	0.0708455	12	1.25223	2.3	2	4.964	235.8	11.9	0.0593217
9	5.2	266	23.4	0.153995	9	2.41298	4.4	3.9	5.2	266.2	9.3	0.146309
10	5.305	291	25.5	0.226219	4	1.43622	2.6	2.3	5.305	291.6	3.6	0.22706
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.624	367	32.2	0.396088	5	3.56252	6.5	5.8	5.624	367.8	4.9	0.411237
12	5.77	402	35.3	0.0515871	6	0.557883	1	0.9	5.77	403	6.5	0.0484795
13	5.85	421	37	0.302364	4	2.43058	4.4	3.9	5.85	421.3	4.4	0.311096
14	5.964	448	39.3	0.551192	4	4.17498	7.6	6.7	5.964	449.5	4.3	0.551156
15	6.065	472	41.4	0.388467	4	2.96526	5.4	4.8	6.065	471.4	4.4	0.376989
16	6.149	492	43.2	0.102621	8	1.35301	2.5	2.2	6.149	489.5	7.7	0.0997144
17	6.491	573	50.3	0.109048	10	1.8905	3.4	3.1	6.491	574.8	9.5	0.112207
18	6.537	584	51.3	0.160013	4	0.732241	1.3	1.2	6.537	584	3.8	0.108048
19	6.623	597	52.4	0.138227	2	0.374887	0.7	0.6	6.623	597.5	1.5	0.140544
20	6.704	608	53.4	0.404361	2	0.925257	1.7	1.5	6.704	608.2	1.7	0.302861
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.799	621	54.5	0.199812	10	4.15267	7.5	6.7	6.799	617	9.6	0.243264
22	7.116	677	59.4	0.0203899	3	0.12253	0.2	0.2	7.116	677.3	3.4	0.0204017
23	7.35	720	63.2	0.321722	6	3.19008	5.8	5.1	7.35	718.6	6	0.30017
24	7.849	821	72.1	0.125424	6	1.45784	2.6	2.4	7.849	822.2	6.4	0.129352
25	8.15	882	77.4	0.119023	24	5.46461	9.9	8.8	8.15	863.6	24	0.128464
26	8.45	949	83.3	0.193138	11	3.36554	6.1	5.4	8.45	948.4	11.1	0.170616
27	8.533	966	84.8	0.0878131	14	1.68501	3.1	2.7	8.533	968.3	13.7	0.0694138
28	8.65	990	86.9	0.102627	7	1.22606	2.2	2	8.65	990.3	7.2	0.095518
29	8.865	1026	90.1	0.0158041	10	0.317291	0.6	0.5	8.865	1025.7	10.2	0.0175363
30	9.3	1099	96.5	0.123908	8	1.53618	2.8	2.5	9.3	1097.4	7.6	0.114618

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.783	80	7	0.0686065	8	0.897039	2.3	1.8	3.783	79.7	7.6	0.0666153
2	4.172	135	11.9	0.00959841	4	0.0676613	0.2	0.1	4.172	137.7	4	0.00946617
3	4.334	158	13.9	0.321356	5	3.12676	8.2	6.3	4.334	157.8	5.4	0.324885
4	4.518	184	16.2	0.0206079	8	0.247769	0.6	0.5	4.518	182.5	8.3	0.0167858
5	4.806	220	19.3	0.0289969	12	0.607526	1.6	1.2	4.806	214.9	11.8	0.0289567
6	4.985	242	21.2	0.0182951	7	0.163418	0.4	0.3	4.985	244.2	6.6	0.0139016
7	5.131	260	22.8	0.0379848	8	0.495103	1.3	1	5.131	256.4	7.6	0.0365163
8	5.323	298	26.2	0.0823699	13	1.7877	4.7	3.6	5.323	296.4	12.8	0.0790298
9	5.389	314	27.6	0.242667	6	2.02349	5.3	4.1	5.389	312.7	5.7	0.201336
10	5.671	382	33.5	0.381526	5	3.68246	9.6	7.4	5.671	382	5.4	0.382943
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.817	417	36.6	0.0566764	8	0.742756	1.9	1.5	5.817	416.9	8.3	0.0507503
12	5.897	436	38.3	0.0595595	7	0.751642	2	1.5	5.897	435.7	7	0.0604076
13	5.987	457	40.1	0.533011	4	3.83545	10	7.7	5.987	457.6	4.3	0.507646
14	6.072	477	41.9	0.386326	4	2.66167	7	5.4	6.072	476.9	4.1	0.370711
15	6.145	494	43.4	0.106436	8	1.48172	3.9	3	6.145	492.2	7.7	0.109134
16	6.465	569	50	0.0136367	2	0.0512299	0.1	0.1	6.465	569.2	2.3	0.0126719
17	6.52	582	51.1	0.175282	3	0.972499	2.5	2	6.52	581.3	3.1	0.178815
18	6.572	592	52	0.0902554	2	0.311186	0.8	0.6	6.572	592.5	2.1	0.0850821
19	6.645	602	52.9	0.333917	2	0.998576	2.6	2	6.645	602.3	2.4	0.237669
20	6.711	611	53.6	0.243061	10	4.46565	11.7	9	6.711	611.9	9.8	0.256878
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.077	672	59	0.0209227	9	0.474959	1.2	1	7.077	664.6	8.5	0.0315067
22	7.266	707	62.1	0.0861326	4	0.618742	1.6	1.2	7.266	707.1	4.2	0.0838239
23	7.799	814	71.5	0.124268	5	1.09716	2.9	2.2	7.799	814.4	5	0.123407
24	8.485	955	83.8	0.118487	29	5.60225	14.6	11.3	8.485	950.4	28.8	0.109589
25	8.868	1026	90.1	0.0619679	10	1.11036	2.9	2.2	8.868	1023.3	9.8	0.0639556

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	39	3.4	0.282134	13	6.2201	19.2	12.8	3.5	40.1	13.3	0.262991
2	4.55	188	16.5	0.232385	7	2.69445	8.3	5.6	4.55	187.5	7.3	0.209155
3	5.2	268	23.6	0.267619	7	3.32484	10.3	6.9	5.2	267.5	7.1	0.262479
4	5.85	426	37.5	0.441229	4	3.61269	11.2	7.5	5.85	426.3	4.4	0.458977
5	6.55	588	51.8	0.313038	7	3.46564	10.7	7.1	6.55	585.7	6.7	0.291448
6	6.85	629	55.4	0.119719	8	1.44352	4.5	3	6.85	628.8	7.6	0.106994
7	7.35	722	63.6	0.293071	5	2.6882	8.3	5.5	7.35	721.5	5.3	0.285481
8	8.15	886	78	0.0894634	10	1.52722	4.7	3.2	8.15	884.7	10	0.0860343
9	8.45	944	83.1	0.156429	7	1.99243	6.2	4.1	8.45	943.6	7.4	0.151271
10	8.65	982	86.4	0.0967439	7	1.06291	3.3	2.2	8.65	981.4	6.5	0.0916514
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1103	97.1	0.280812	9	4.29837	13.3	8.9	9.3	1099.8	9.4	0.25894

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.769	76	6.7	0.0540456	7	0.685824	1.8	1.4	3.769	75.4	7.4	0.0525324
2	4.184	134	11.8	0.0165961	9	0.21753	0.6	0.4	4.184	133.9	8.9	0.0137523
3	4.306	151	13.3	0.333807	5	2.9057	7.7	5.8	4.306	150.9	4.9	0.334991
4	4.478	175	15.4	0.0218642	11	0.325986	0.9	0.7	4.478	171.9	10.8	0.0170457
5	4.665	199	17.5	0.0224345	6	0.214798	0.6	0.4	4.665	198.6	6.3	0.019388
6	4.739	208	18.3	0.0275356	5	0.232574	0.6	0.5	4.739	208.2	5.2	0.0254754
7	5.052	246	21.7	0.0517129	24	1.56246	4.1	3.1	5.052	244.1	24	0.0367308
8	5.275	282	24.8	0.0942822	10	1.34319	3.5	2.7	5.275	281.5	10	0.0754812
9	5.346	299	26.3	0.277495	4	2.0611	5.4	4.1	5.346	297.5	4.4	0.266662
10	5.637	369	32.5	0.445756	5	3.69474	9.7	7.4	5.637	368.8	4.6	0.453833
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.775	402	35.4	0.0623746	6	0.681085	1.8	1.4	5.775	402.6	6.3	0.0610179
12	5.867	424	37.3	0.0672616	8	0.927239	2.4	1.9	5.867	422.8	8	0.0656373
13	5.958	445	39.2	0.582466	4	3.91482	10.3	7.9	5.958	445.9	3.9	0.567838
14	6.049	466	41	0.424852	4	2.77959	7.3	5.6	6.049	466.1	3.9	0.405954
15	6.101	478	42.1	0.121816	9	1.93819	5.1	3.9	6.101	481.9	8.6	0.126685
16	6.517	574	50.5	0.234018	5	1.78817	4.7	3.6	6.517	573.6	4.8	0.210921
17	6.575	585	51.5	0.182785	3	0.874291	2.3	1.8	6.575	584.9	3.1	0.160563
18	6.648	595	52.4	0.367613	3	1.43083	3.8	2.9	6.648	594.9	3.3	0.245658
19	6.749	609	53.6	0.201596	10	4.46247	11.8	9	6.749	603.6	9.8	0.257417
20	7.073	664	58.5	0.0168095	3	0.0909725	0.2	0.2	7.073	664	3.3	0.0155316
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.268	700	61.6	0.08644	4	0.637864	1.7	1.3	7.268	700.6	4.1	0.0888541
22	7.782	804	70.8	0.115937	5	0.972949	2.6	2	7.782	804.1	4.8	0.115271
23	8.462	945	83.2	0.0855205	19	2.85441	7.5	5.7	8.462	943.7	19.2	0.0840815
24	8.581	969	85.3	0.0353373	8	0.308201	0.8	0.6	8.581	970.4	8.4	0.0206494
25	8.814	1015	89.3	0.0587521	10	1.05077	2.8	2.1	8.814	1014.5	10.1	0.058619

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	38	3.3	0.27887	12	5.43593	17.8	12.6	3.5	39	11.8	0.259236
2	4.55	182	16	0.251124	6	2.26985	7.5	5.2	4.55	182.3	5.9	0.218751
3	5.2	260	22.9	0.299799	6	3.05688	10	7.1	5.2	259.3	5.9	0.294191
4	5.85	414	36.4	0.437027	4	3.46399	11.4	8	5.85	414.4	4.3	0.453455
5	6.55	575	50.6	0.359341	5	3.20233	10.5	7.4	6.55	573.8	5.3	0.341648
6	6.85	617	54.3	0.125702	7	1.43368	4.7	3.3	6.85	617.1	7.4	0.109917
7	7.35	708	62.3	0.319355	5	2.64237	8.7	6.1	7.35	707.6	4.8	0.312282
8	8.15	874	76.9	0.0981397	10	1.65096	5.4	3.8	8.15	872.7	9.7	0.0957242
9	8.45	941	82.8	0.14294	7	1.64807	5.4	3.8	8.45	940.7	6.6	0.14154
10	8.65	984	86.6	0.10027	6	1.0005	3.3	2.3	8.65	983.1	5.8	0.0970891
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1117	98.3	0.406184	8	4.65156	15.3	10.8	9.3	1115.4	7.5	0.348777

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 113

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 17.600 4.550 10.200 5.200 11.800 5.850 11.500 6.550 10.500 6.850 3.800 7.350 8.300 8.150 5.800 8.450 6.400 8.650 3.100 9.300 11.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 5.100 3.734 0.900 4.131 0.300 4.259 4.700 4.429 0.700 4.550 2.700 4.672 1.100 4.964 2.300 5.200 4.400 5.305 2.600 5.624 6.500 5.770 1.000 5.850 4.400 5.964 7.600 6.065 5.400 6.149 2.500 6.491 3.400 6.537 1.300 6.623 0.700 6.704 1.700 6.799 7.500 7.116 0.200 7.350 5.800 7.849 2.600 8.150 9.900 8.450 6.100 8.533 3.100 8.650 2.200 8.865 0.600 9.300 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 30 Return to Lane Navigator
3.783 2.300 4.172 0.200 4.334 8.200 4.518 0.600 4.806 1.600 4.985 0.400 5.131 1.300 5.323 4.700 5.389 5.300 5.671 9.600 5.817 1.900 5.897 2.000 5.987 10.000 6.072 7.000 6.145 3.900 6.465 0.100 6.520 2.500 6.572 0.800 6.645 2.600 6.711 11.700 7.077 1.200 7.266 1.600 7.799 2.900 8.485 14.600 8.868 2.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 25 Return to Lane Navigator
3.500 19.200 4.550 8.300 5.200 10.300 5.850 11.200 6.550 10.700 6.850 4.500 7.350 8.300 8.150 4.700 8.450 6.200 8.650 3.300 9.300 13.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
3.769 1.800 4.184 0.600 4.306 7.700 4.478 0.900 4.665 0.600 4.739 0.600 5.052 4.100 5.275 3.500 5.346 5.400 5.637 9.700 5.775 1.800 5.867 2.400 5.958 10.300 6.049 7.300 6.101 5.100 6.517 4.700 6.575 2.300 6.648 3.800 6.749 11.800 7.073 0.200 7.268 1.700 7.782 2.600 8.462 7.500 8.581 0.800 8.814 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 19 PeakCount: 25 Return to Lane Navigator
3.500 17.800 4.550 7.500 5.200 10.000 5.850 11.400 6.550 10.500 6.850 4.700 7.350 8.700 8.150 5.400 8.450 5.400 8.650 3.300 9.300 15.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 20 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Caribbean Red Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Caribbean Red Snapper ---- Go to Display Gel Lane Navigator.