RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Chilipepper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes goodei
FDA Seafood List Common Name: Chilipepper
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19981215gel05
Unique Web Code: cih01Srr
Tissue Aliquot Code: sebagood E97-002 #02m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	31	4	0.0815574	3	0.454304	6.1	4.3	4.55	31.7	3.1	0.0815229
2	5.2	122	15.6	0.0753435	4	0.549202	7.3	5.3	5.2	122	4.3	0.0723001
3	5.85	227	29	0.0626912	3	0.301764	4	2.9	5.85	226.6	2.8	0.061313
4	6.55	369	47.2	0.154085	6	1.41596	18.9	13.6	6.55	367.8	5.6	0.143691
5	6.85	400	51.2	0.0289486	5	0.215473	2.9	2.1	6.85	399.8	4.5	0.0269804
6	7.35	467	59.7	0.0605183	3	0.346326	4.6	3.3	7.35	466.7	3.2	0.0604002
7	8.15	587	75.1	0.117673	6	1.1848	15.8	11.3	8.15	586.1	5.8	0.114809
8	8.45	649	83	0.142947	7	1.67518	22.3	16	8.45	648.6	6.9	0.137782
9	8.65	704	90	0.122056	4	0.789372	10.5	7.6	8.65	703.4	3.8	0.117925
10	9.3	757	96.8	0.0692783	5	0.575774	7.7	5.5	9.3	756.4	4.8	0.0671752

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.521	24	3.1	2.39192	3	14.8913	23.8	17.7	4.521	25.4	3.1	2.68608
2	4.745	55	7.1	0.226241	8	3.1798	5.1	3.8	4.745	57.5	8.4	0.212837
3	5.92	235	30.1	2.34303	3	13.9529	22.3	16.6	5.92	236.2	3	2.6146
4	5.984	248	31.8	2.30931	3	11.0077	17.6	13.1	5.984	248.7	2.5	2.4791
5	6.078	267	34.2	0.0172671	2	0.0875852	0.1	0.1	6.078	268.3	1.9	0.0256334
6	6.143	280	35.9	0.221422	3	0.994646	1.6	1.2	6.143	279.2	2.7	0.208155
7	6.178	287	36.8	0.0940886	6	0.779954	1.2	0.9	6.178	287.3	6.5	0.067863
8	6.217	295	37.8	2.18212	1	5.78333	9.2	6.9	6.217	295.1	1.5	2.23912
9	6.346	321	41.2	0.145159	5	1.10771	1.8	1.3	6.346	320.9	4.9	0.126724
10	6.386	329	42.2	0.0846656	6	0.673489	1.1	0.8	6.386	328.7	5.6	0.0679989
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.471	346	44.4	0.224251	3	1.21319	1.9	1.4	6.471	346.5	3.1	0.223913
12	6.51	354	45.4	0.299568	3	1.72874	2.8	2.1	6.51	353.8	3.5	0.27962
13	7.032	420	53.8	0.0248965	7	0.259794	0.4	0.3	7.032	422.6	6.8	0.021544
14	7.474	483	61.9	0.184945	10	3.12449	5	3.7	7.474	484.7	9.8	0.179785
15	7.641	508	65.1	1.48574	1	3.03438	4.8	3.6	7.641	508	1.2	1.40926
16	9.174	742	95.1	0.0324815	5	0.288678	0.5	0.3	9.174	743.1	5.3	0.0307112
17	9.354	757	97.1	0.0629125	5	0.520923	0.8	0.6	9.354	756	4.6	0.0634977

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	26	3.3	0.0661777	4	0.483912	7	5.2	4.55	26.2	4.2	0.0656233
2	5.2	115	14.8	0.0620351	6	0.648326	9.3	6.9	5.2	112.9	6.3	0.0585174
3	5.85	216	27.7	0.059113	4	0.386628	5.6	4.1	5.85	216	3.8	0.0574762
4	6.55	356	45.7	0.121518	7	1.38635	19.9	14.8	6.55	354.6	7.1	0.109625
5	6.85	390	50.1	0.0225709	7	0.214624	3.1	2.3	6.85	390.2	6.5	0.0185532
6	7.35	463	59.4	0.0458503	3	0.280783	4	3	7.35	462.8	3.5	0.0453
7	8.15	582	74.7	0.124665	5	1.10057	15.8	11.7	8.15	581.5	5.1	0.12184
8	8.45	642	82.4	0.105371	7	1.25382	18	13.4	8.45	641.1	6.9	0.102141
9	8.65	694	89.1	0.0944608	4	0.672561	9.7	7.2	8.65	693.2	4.2	0.0905437
10	9.3	749	96.1	0.0777696	4	0.534797	7.7	5.7	9.3	748.6	3.9	0.0768501

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.573	30	3.8	1.70606	4	12.6031	24.2	21.4	4.573	30.4	4.4	1.62461
2	4.773	59	7.6	0.104875	8	1.45725	2.8	2.5	4.773	61.1	8.5	0.0967673
3	5.2	121	15.5	0.0206084	6	0.188667	0.4	0.3	5.2	119.8	5.6	0.0191433
4	5.792	210	26.9	0.0126095	3	0.0561101	0.1	0.1	5.792	208.9	2.8	0.0113614
5	5.881	225	28.8	1.76783	4	10.4924	20.1	17.8	5.881	224.8	3.8	1.57646
6	5.95	239	30.6	1.89198	3	10.5546	20.3	17.9	5.95	238.7	3.4	1.76445
7	6.039	257	32.9	0.0152956	18	0.606117	1.2	1	6.039	239	18	0.0189984
8	6.098	269	34.5	0.106581	6	1.0186	2	1.7	6.098	270.8	5.9	0.0967015
9	6.191	288	36.9	0.854537	3	3.81683	7.3	6.5	6.191	286.2	3.2	0.683065
10	6.319	314	40.3	0.0753039	6	0.831961	1.6	1.4	6.319	315.4	6.4	0.0737312
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.461	343	44	0.142636	5	1.12413	2.2	1.9	6.461	342.5	5.1	0.125108
12	6.501	351	45	0.144598	4	0.877193	1.7	1.5	6.501	351.5	3.8	0.12899
13	6.85	394	50.5	0.0268325	4	0.142219	0.3	0.2	6.85	393.9	3.6	0.0220049
14	7.017	418	53.6	0.0144193	6	0.119234	0.2	0.2	7.017	419.4	5.6	0.0119691
15	7.35	466	59.7	0.0145896	5	0.107339	0.2	0.2	7.35	465.7	5	0.0120512
16	7.543	493	63.2	0.0972008	8	1.4074	2.7	2.4	7.543	493	8.5	0.0935777
17	7.664	510	65.4	0.262282	3	1.40741	2.7	2.4	7.664	509.7	3.1	0.258455
18	8.15	578	74.1	0.220134	10	3.72346	7.1	6.3	8.15	575.5	10.5	0.200101
19	8.45	646	82.8	0.0399131	5	0.375354	0.7	0.6	8.45	645.1	5.3	0.040053
20	8.65	695	89.1	0.0375219	7	0.432937	0.8	0.7	8.65	695	7	0.0351152
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.152	739	94.7	0.0284023	5	0.240733	0.5	0.4	9.152	738.4	5.1	0.0265803
22	9.3	752	96.4	0.0485685	6	0.528868	1	0.9	9.3	751.2	5.9	0.0506662

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.533	24	3.1	2.38952	3	16.0556	25.2	19.9	4.533	25.2	3.4	2.67908
2	4.732	52	6.7	0.273506	5	2.35512	3.7	2.9	4.732	52.8	5.3	0.252714
3	5.863	223	28.7	2.35605	3	12.8686	20.2	15.9	5.863	224.7	2.8	2.59061
4	5.935	238	30.6	2.34448	3	12.6628	19.9	15.7	5.935	238.7	2.8	2.51046
5	6.032	258	33.2	0.0563739	18	0.796697	1.2	1	6.032	250.4	17.9	0.0250553
6	6.1	272	35	0.213203	4	1.20712	1.9	1.5	6.1	271.6	3.6	0.190845
7	6.182	289	37.1	1.63737	2	4.78483	7.5	5.9	6.182	288.6	1.5	1.79558
8	6.318	317	40.7	0.128298	5	0.984744	1.5	1.2	6.318	317.1	4.6	0.119745
9	6.356	325	41.8	0.0865367	4	0.546783	0.9	0.7	6.356	325.3	3.7	0.0826703
10	6.463	347	44.6	0.227607	4	1.44272	2.3	1.8	6.463	346.2	3.7	0.21762
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.502	355	45.6	0.270134	4	1.75428	2.8	2.2	6.502	354.5	3.8	0.258813
12	7.013	419	53.9	0.0212578	5	0.166394	0.3	0.2	7.013	420.1	4.9	0.019264
13	7.54	492	63.2	0.190548	11	3.50618	5.5	4.3	7.54	488.6	10.6	0.187097
14	7.658	509	65.4	1.24224	2	3.82771	6	4.7	7.658	508.6	1.7	1.30493
15	9.179	742	95.4	0.0358175	4	0.261711	0.4	0.3	9.179	742.3	4.2	0.0354476
16	9.329	755	97	0.0570435	5	0.525385	0.8	0.7	9.329	754.6	5.3	0.0563911

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	25	3.2	0.0717278	3	0.414827	4.3	3.1	4.55	25.3	3.4	0.0684573
2	5.2	114	14.7	0.0807581	5	0.678146	7	5	5.2	114.2	4.9	0.0780975
3	5.85	221	28.5	0.082798	3	0.422374	4.3	3.1	5.85	221	3	0.0781939
4	6.55	368	47.5	0.223028	5	1.90719	19.6	14.2	6.55	367.8	5.1	0.209606
5	6.85	399	51.5	0.0430595	6	0.41408	4.2	3.1	6.85	400.3	5.8	0.0401885
6	7.35	462	59.6	0.0941616	4	0.602649	6.2	4.5	7.35	461.8	3.6	0.0931816
7	8.15	581	75	0.155156	6	1.55116	15.9	11.5	8.15	580.7	5.9	0.148684
8	8.45	641	82.7	0.15756	8	2.07923	21.3	15.5	8.45	640	7.8	0.150598
9	8.65	696	89.8	0.15126	4	0.974046	10	7.3	8.65	695.7	3.8	0.143629
10	9.3	752	97	0.0896058	5	0.700604	7.2	5.2	9.3	751.7	4.5	0.0874259

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 85

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.100 5.200 7.300 5.850 4.000 6.550 18.900 6.850 2.900 7.350 4.600 8.150 15.800 8.450 22.300 8.650 10.500 9.300 7.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.521 23.800 4.745 5.100 5.920 22.300 5.984 17.600 6.078 0.100 6.143 1.600 6.178 1.200 6.217 9.200 6.346 1.800 6.386 1.100 6.471 1.900 6.510 2.800 7.032 0.400 7.474 5.000 7.641 4.800 9.174 0.500 9.354 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 17 Return to Lane Navigator
4.550 7.000 5.200 9.300 5.850 5.600 6.550 19.900 6.850 3.100 7.350 4.000 8.150 15.800 8.450 18.000 8.650 9.700 9.300 7.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.573 24.200 4.773 2.800 5.200 0.400 5.792 0.100 5.881 20.100 5.950 20.300 6.039 1.200 6.098 2.000 6.191 7.300 6.319 1.600 6.461 2.200 6.501 1.700 6.850 0.300 7.017 0.200 7.350 0.200 7.543 2.700 7.664 2.700 8.150 7.100 8.450 0.700 8.650 0.800 9.152 0.500 9.300 1.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.533 25.200 4.732 3.700 5.863 20.200 5.935 19.900 6.032 1.200 6.100 1.900 6.182 7.500 6.318 1.500 6.356 0.900 6.463 2.300 6.502 2.800 7.013 0.300 7.540 5.500 7.658 6.000 9.179 0.400 9.329 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 4.300 5.200 7.000 5.850 4.300 6.550 19.600 6.850 4.200 7.350 6.200 8.150 15.900 8.450 21.300 8.650 10.000 9.300 7.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Chilipepper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Chilipepper ---- Go to Display Gel Lane Navigator.