RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Blacktip Shark ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Carcharhinus limbatus
FDA Seafood List Common Name: Blacktip Shark
FDA Seafood List Market Name: Shark
Unique IEF Gel Code: 19950830gel01
Unique Web Code: bth02Srr
Tissue Aliquot Code: carclimb I94-002 #05 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	32	4	0.051708	4	0.360017	14.1	9.5	4.55	31.8	4	0.0510247
2	5.2	121	15.2	0.0205096	6	0.206144	8.1	5.4	5.2	120.7	6.1	0.019008
3	5.85	226	28.5	0.0359047	5	0.274117	10.8	7.2	5.85	225.7	4.6	0.0337421
4	6.55	345	43.5	0.0216514	11	0.39965	15.7	10.5	6.55	341.6	11	0.0204255
5	7.35	449	56.5	0.00383079	4	0.0248191	1	0.7	7.35	450.7	4.1	0.00338677
6	8.15	575	72.4	0.0317175	10	0.477161	18.7	12.6	8.15	574	9.6	0.0279944
7	8.45	644	81.1	0.0281953	7	0.318236	12.5	8.4	8.45	642.3	6.8	0.026367
8	8.65	696	87.7	0.0256529	4	0.175629	6.9	4.6	8.65	695.8	4.3	0.0232306
9	9.3	763	96.1	0.0310677	6	0.313551	12.3	8.3	9.3	761.1	6	0.0294177

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.991	252	31.7	0.00617243	4	0.0345653	0.4	0.3	5.991	251.2	4.3	0.00455537
2	6.037	260	32.7	0.00641114	7	0.0560304	0.6	0.5	6.037	259.8	6.6	0.00478492
3	6.254	297	37.4	0.0308703	14	0.695684	7.8	5.8	6.254	300.2	14	0.0279986
4	6.459	332	41.8	0.400182	3	1.87816	21	15.5	6.459	331.9	2.8	0.385017
5	6.547	347	43.6	0.0709926	11	1.03174	11.5	8.5	6.547	343.9	10.5	0.05533
6	6.685	365	45.9	0.0572509	4	0.374253	4.2	3.1	6.685	364.8	3.9	0.0537267
7	6.971	402	50.6	0.00360702	3	0.0536568	0.6	0.4	6.971	400	3.4	0.00882448
8	7.072	415	52.2	0.00383414	6	0.038727	0.4	0.3	7.072	412.7	5.9	0.00368003
9	7.319	447	56.2	0.0288718	14	0.677156	7.6	5.6	7.319	445.7	13.8	0.0277595
10	7.495	473	59.5	0.0138909	3	0.0436245	0.5	0.4	7.495	473	2.5	0.00984171
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.607	490	61.6	0.0287841	6	0.252187	2.8	2.1	7.607	490.3	6.4	0.0220733
12	8.282	603	75.8	0.118299	10	2.0647	23.1	17.1	8.282	602.3	10.4	0.112325
13	9.11	742	93.3	0.0553876	13	1.13176	12.7	9.4	9.11	736.8	12.5	0.0509217
14	9.291	761	95.7	0.0759111	5	0.613238	6.9	5.1	9.291	761.1	4.9	0.0709685

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	30	3.8	0.0488998	5	0.420255	15.9	10.7	4.55	30.8	5.1	0.0466016
2	5.2	130	16.3	0.0183849	10	0.302097	11.4	7.7	5.2	132.4	10.2	0.0167837
3	5.85	230	28.9	0.0471818	4	0.290963	11	7.4	5.85	230	3.6	0.0453457
4	6.55	350	44	0.0391457	8	0.44689	16.9	11.4	6.55	349.4	7.6	0.0333533
5	7.35	453	56.9	0.00699355	10	0.0884815	3.3	2.3	7.35	451.6	9.6	0.00521852
6	8.15	570	71.6	0.0313097	7	0.35661	13.5	9.1	8.15	569.7	6.7	0.0300053
7	8.45	640	80.4	0.0237981	9	0.341564	12.9	8.7	8.45	637.8	8.6	0.0223411
8	8.65	691	86.8	0.0240632	5	0.191619	7.2	4.9	8.65	691.2	4.7	0.0230826
9	9.3	761	95.6	0.0169972	9	0.205507	7.8	5.2	9.3	759.8	8.6	0.0135528

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	42	5.3	0.0376897	6	0.349542	5.9	4.5	4.55	42.1	5.6	0.035033
2	5.2	139	17.4	0.0171673	6	0.186821	3.1	2.4	5.2	138.1	6.4	0.016374
3	5.85	240	30	0.0324524	4	0.214211	3.6	2.8	5.85	239.7	3.8	0.031503
4	6.264	305	38.2	0.0172162	9	0.224635	3.8	2.9	6.264	307.3	9.4	0.013522
5	6.486	340	42.6	0.290587	3	1.32052	22.2	17.1	6.486	340.1	2.7	0.274446
6	6.587	355	44.4	0.0516817	10	0.86543	14.5	11.2	6.587	352	10.2	0.0479068
7	6.714	372	46.6	0.0300056	3	0.169715	2.8	2.2	6.714	372.7	3.4	0.028239
8	7.088	422	52.8	0.003959	5	0.0337128	0.6	0.4	7.088	423.1	5.3	0.00359155
9	7.223	440	55.1	0.00806974	11	0.188984	3.2	2.4	7.223	446	10.8	0.00987279
10	7.35	457	57.2	0.050607	6	0.435231	7.3	5.6	7.35	457	5.6	0.0434737
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.558	487	61	0.00781587	4	0.0535945	0.9	0.7	7.558	487.2	4.4	0.00691796
12	7.669	503	63	0.0134682	7	0.138833	2.3	1.8	7.669	502.6	6.7	0.0117638
13	8.301	612	76.6	0.0533233	10	0.864992	14.5	11.2	8.301	613.7	9.8	0.0496334
14	8.45	651	81.5	0.0157227	8	0.195619	3.3	2.5	8.45	649.5	8.1	0.0136225
15	8.65	698	87.4	0.0184934	4	0.132033	2.2	1.7	8.65	697.7	4.3	0.0172846
16	9.125	746	93.4	0.0146645	7	0.185521	3.1	2.4	9.125	745.4	7.1	0.0148255
17	9.284	762	95.4	0.0573169	4	0.39558	6.6	5.1	9.284	761.7	3.9	0.0577592

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	6.017	255	32.1	0.00950068	9	0.128288	1.8	1.4	6.017	256.6	8.9	0.00811058
2	6.277	298	37.5	0.0353829	13	0.683106	9.8	7.3	6.277	299.2	12.7	0.030229
3	6.489	333	41.9	0.372069	3	1.79624	25.7	19.3	6.489	332.8	2.9	0.352501
4	6.588	348	43.8	0.048828	11	0.839915	12	9	6.588	345.1	11.3	0.0419717
5	6.718	365	45.9	0.0437053	4	0.276055	3.9	3	6.718	365.1	3.9	0.0401034
6	6.994	401	50.4	0.00648329	5	0.0524858	0.8	0.6	6.994	399.1	4.9	0.00607838
7	7.055	409	51.4	0.00600748	3	0.0267515	0.4	0.3	7.055	408.9	2.9	0.00517624
8	7.193	427	53.7	0.00704254	7	0.0737019	1.1	0.8	7.193	427.3	6.7	0.00624548
9	7.339	446	56.1	0.0256668	8	0.353501	5.1	3.8	7.339	444.3	7.9	0.0252614
10	7.393	454	57.1	0.0231557	6	0.17397	2.5	1.9	7.393	455.4	5.8	0.0170058
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.526	474	59.6	0.00875978	3	0.0481459	0.7	0.5	7.526	474.9	3.2	0.00857506
12	7.646	492	61.9	0.0184269	4	0.121702	1.7	1.3	7.646	491.9	3.9	0.0175515
13	8.222	585	73.6	0.0788228	14	1.82355	26.1	19.6	8.222	583.5	13.9	0.0742135
14	9.158	744	93.6	0.022986	3	0.131673	1.9	1.4	9.158	743.3	3.2	0.0235732
15	9.288	758	95.3	0.058033	4	0.460113	6.6	4.9	9.288	757.8	4.5	0.0583024

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	27	3.4	0.0482457	4	0.361171	13	9.2	4.55	27.6	4.3	0.0472072
2	5.2	119	14.9	0.0165427	7	0.19143	6.9	4.9	5.2	119.2	6.8	0.0159891
3	5.85	220	27.6	0.0315135	4	0.225776	8.1	5.8	5.85	219.9	4.3	0.0298153
4	6.55	341	42.8	0.038835	9	0.561283	20.2	14.3	6.55	338.3	9.4	0.0338591
5	7.35	443	55.7	0.0152631	6	0.138	5	3.5	7.35	442.4	6.1	0.0128476
6	8.15	568	71.4	0.0378463	8	0.487383	17.5	12.5	8.15	566.7	7.6	0.0362892
7	8.45	634	79.6	0.031726	7	0.353676	12.7	9	8.45	633.7	6.8	0.0295391
8	8.65	685	86.1	0.021771	6	0.19737	7.1	5	8.65	685.1	5.6	0.0197968
9	9.3	760	95.5	0.0282951	6	0.266534	9.6	6.8	9.3	759.9	6	0.0249668

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 73

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 14.100 5.200 8.100 5.850 10.800 6.550 15.700 7.350 1.000 8.150 18.700 8.450 12.500 8.650 6.900 9.300 12.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
5.991 0.400 6.037 0.600 6.254 7.800 6.459 21.000 6.547 11.500 6.685 4.200 6.971 0.600 7.072 0.400 7.319 7.600 7.495 0.500 7.607 2.800 8.282 23.100 9.110 12.700 9.291 6.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 14 Return to Lane Navigator
4.550 15.900 5.200 11.400 5.850 11.000 6.550 16.900 7.350 3.300 8.150 13.500 8.450 12.900 8.650 7.200 9.300 7.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 5.900 5.200 3.100 5.850 3.600 6.264 3.800 6.486 22.200 6.587 14.500 6.714 2.800 7.088 0.600 7.223 3.200 7.350 7.300 7.558 0.900 7.669 2.300 8.301 14.500 8.450 3.300 8.650 2.200 9.125 3.100 9.284 6.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 17 Return to Lane Navigator
6.017 1.800 6.277 9.800 6.489 25.700 6.588 12.000 6.718 3.900 6.994 0.800 7.055 0.400 7.193 1.100 7.339 5.100 7.393 2.500 7.526 0.700 7.646 1.700 8.222 26.100 9.158 1.900 9.288 6.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 15 Return to Lane Navigator
4.550 13.000 5.200 6.900 5.850 8.100 6.550 20.200 7.350 5.000 8.150 17.500 8.450 12.700 8.650 7.100 9.300 9.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Blacktip Shark ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Blacktip Shark ---- Go to Display Gel Lane Navigator.