BNL STEM 1

V0 40 keV Probe 0.25 nm Beam Intensity 10³ el/pixel Dark Field 15 - 40 mR or 40 - 200 mR Specimens on X,Y Stage 6 Samples in Vacuum at 20° or -180°C Data Aquisition Windows PC

Probe

The STEM is operated at 40 keV with a probe focussed to 0.25 nm. The sample is maintained at -150°C to eliminate contamination and to reduce mass loss.

Scan

The scan raster is 512 x 512 points with a dwell time of 30 µs per pixel (8.5 sec per scan). For most mass measurements, the pixels are separated by 1 nm giving a scan width of 0.512 µm. These scanning conditions provide the best compromise between resolution of the specimen and radiation damage to it.

Detectors

Electrons elastically scattered through large angles (40-200 mR), through small angles (15-40 mR), or unscattered (0-12 mR) are collected on separate detectors consisting of scintillators and photomultipliers. Both large and small angle signals (normalized by the total beam current) are recorded digitally. The dose is kept below 1000 electrons/nm² to assure that mass loss from radiation damage is less than 2.5% at the -150°C specimen temperature employed.

Image

Digital images are recorded with the STEM from the annular dark field detectors. Areas of the grid to be used for mass measurements are selected for clean background and adequate numbers of both TMV and specimen particles. The background is computed in clear areas in each image, and the intensity minus background is summed over each particle and multiplied by a calibration factor to give a mass value.

Calibration

The STEM calibration factor is checked in each image using TMV which is included as an internal control. The TMV also serves as a qualitative control for the whole process of specimen preparation. If the TMV looks good, the specimen has a chance of being good, but if it looks bad, some step in the specimen preparation has gone wrong. Different kinds of mass measurements can be obtained from this data. The total mass of the individual particles can be used to determine their oligomeric state. For filamentous specimens, the mass per length can be used to determine the spacing of repeating units. Similarly, the mass per area can be a useful measurement on two-dimensional arrays.

Updated 4 Oct 2007	Security Notice   Webteam   Site Map   STEM   Biology   BNL