Volume 9, Number 8, August 2003

NmcA Carbapenem-hydrolyzing Enzyme in Enterobacter cloacae in North America

Sudha Pottumarthy,* Ellen Smith Moland,† Stefan Juretschko,* Susan R. Swanzy,* Kenneth S. Thomson,† and Thomas R. Fritsche*
*University of Washington School of Medicine, Seattle, Washington, USA: and †Creighton University Medical Center, Omaha, Nebraska, USA

Figure 3. Analytical isoelectric focusing (IEF) of serially diluted broth induced enzymes. The isoelectric points (pIs) of the two prominent bands midway and at the bottom of the gel are 6.9 and >9.0. Lanes 1–3: uninduced enzyme serially diluted 1:1, 1:2, and 1:4. Lanes 4–6: enzyme induced with 8 mg/mL of cefoxitin serially diluted 1:1, 1:2, and 1:4. Lanes 7–9: enzyme induced with 4 mg/mL of imipenem serially diluted 1:1, 1:2 and 1:4. Lane 10: NOR-1 control, induced with 16 mg/mL of cefoxitin.

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This page last reviewed July 29, 2003

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention