Project Number: 5335-21000-031-02
Project Type: Reimbursable

Start Date: Oct 01, 2006
End Date: Sep 30, 2009

Objective:
Produce and characterize transgenic canola with pollen-specific recombinases in which the T-DNA includes recognition sites flanking genes that code for recombinases and fluorescent proteins that serve as whole plant and pollen markers.

Approach:
Seven plant transformation vectors will be produced and tested in transgenic canola: 1. loxP recognition sites and Cre recombinase; 2. Fused loxP/FRT recognition sites and CRE recombinase; 3. ParA recongition sites and ParA recombinase system; 4. CinH recongition sites and CinH recombinase system; 5. Tn50-53 recongition sites and Tn5053 recombinase system; 6. Tn1721 recongition sites and Tn1721 recombinase system; and 7. Control with loxP recognition sites with no recombinase cassette (positive control). BSL-1; 7/3/06. Documents Reimbursable with University of Tennessee. Log 31141.