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LOW PATHOGENIC "NORTH AMERICAN" H5N1 AVIAN INFLUENZA STRAIN IN WILD BIRDS
PRESUMPTIVE AND CONFIRMED TEST RESULTS
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DATE SAMPLE
COLLECTED
COUNTY,
STATE
WILD BIRD
SPECIES SAMPLED
SAMPLE
STRATEGY1
COLLECTING
ENTITY
NATIONAL VETERINARY SERVICES LABORATORIES
PRESUMPTIVE POSITIVE TEST RESULTS
NATIONAL VETERINARY SERVICES LABORATORIES
CONFIRMATORY TEST RESULTS
POSITIVE2 H5
POSITIVE N1
INITIAL GENETIC3
SEQUENCING
VIRUS
ISOLATION4
PATHOGENICITY5
12/29/08 Goshen, WY Canada goose Hunter killed USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI Testing underway Testing underway
12/11/08 Barton, KS Mallard Hunter killed Kansas Dept. of Wildlife & Parks Yes Yes Not related to HPAI H5N1; Suspected LPAI H6N1 Not applicable
11/21/08 Wabasha, MN Mallard Hunter killed MN Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI Testing underway Testing underway
11/12/08 Clark, WA Northern Shoveler Hunter killed USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI Testing underway Testing underway
11/6/08 Columbia, OR American Wigeon Hunter killed USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI Testing underway Testing underway
11/4/08 Stoddard, MO Northern pintail Hunter killed MO Dept. of Conservation Yes Yes No readable sequence; suspected LPAI Testing underway Testing underway
11/3/08 Saline, MO Mallard Hunter killed USFWS Yes Yes Not related to HPAI H5N1; Suspected LPAI H6N2 Not applicable
11/1/08 Butte, CA Northern pintail Hunter killed CA Dept. of Fish and Game Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
10/26/08 Mason, IL Northern pintail Hunter killed IL Dept of Natural Resources/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI Testing underway Testing underway
10/26/08 Saint Clair, MI Northern Northern pintail Hunter killed MI Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI Testing underway Testing underway
10/25/08 Grundy, IL Mallard Hunter killed USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI Testing underway Testing underway
10/19/08 Carroll, IL American Green-winged teal Hunter killed USDA Yes Yes No readable sequence; suspected LPAI Testing underway Testing underway
9/29/08 Saginaw, MI Mallard Live birds USFWS Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
9/20/08 Butler, IA Mallard Hunter killed USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
9/18/08 Monmouth, NJ Mallard Live birds New Jersey Division of Fish and Wildlife/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
9/18/08 Brown, SD Northern pintail Live birds USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No AI virus isolated Not applicable
9/5/08 Crawford, PA Mallard Live birds PA Game Commission Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
9/5/08 Marquette, WI Mallard Live birds WI DNR Yes Yes Not related to HPAI H5N1; Suspected LPAI Testing underway Testing underway
8/25/08 Erie, PA Mallard Live birds PA Game Commission Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
8/25/08 Crawford, PA Mallard (#1) Live birds PA Game Commission Yes Yes Not related to HPAI H5N1; Suspected LPAI H3N1 Not applicable
8/25/08 Crawford, PA Mallard (#2) Live birds PA Game Commission Yes Yes Not related to HPAI H5N1; Suspected LPAI H3N2 Not applicable
8/18/08 Marathon, WI Mallard (#1) Live birds Wisconsin DNR Yes Yes Not related to HPAI H5N1; Suspected LPAI H6N1 Not applicable
8/18/08 Marathon, WI Mallard (#2) Live birds Wisconsin DNR Yes Yes Not related to HPAI H5N1; Suspected LPAI No AI virus isolated Not applicable
1/29/08 Jefferson, AR Unknown Environmental USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI H2N9 Not applicable
11/20/07 Kent, DE American black duck Hunter killed DE Dept. of Natural Resources and Environmental Control Yes Yes Not related to HPAI H5N1; Suspected LPAI No AI virus isolated Not applicable
11/20/07 Socorro, NM Northern pintail duck Live birds NM Dept. of Fish and Game Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
11/19/07 Kent, DE Mallard (#1) Hunter killed DE Dept. of Natural Resources and Environmental Control Yes Yes Not related to HPAI H5N1; Suspected LPAI H1N1 Not applicable
11/19/07 Kent, DE Mallard (#2) Hunter killed DE Dept. of Natural Resources and Environmental Control Yes Yes Not related to HPAI H5N1; Suspected LPAI No AI virus isolated Not applicable
11/16/07 Ottawa, OH Mallard Hunter killed Ohio Division of Wildlife Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
11/11/07 Davis, UT Northern pintail duck Hunter killed Utah Division of Wildlife Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
11/4/07 Tuscola, MI American black duck Hunter killed MI Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI N1 virus isolated; H-type unidentifiable Not applicable
10/31/07 Saint Clair, MI American black duck Hunter killed MI Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
10/30/07 Bay, MI Mallard Hunter killed MI Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI N1 virus isolated; H-type unidentifiable (H5 negative) Not applicable
10/29/07 Erie, PA Mallard Hunter killed USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI H3N2 Not applicable
10/29/07 Divide, ND Lesser snow goose Hunter killed USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
10/13/07 Middlesex, NJ Green-winged teal Hunter killed New Jersey Division of Fish and Wildlife/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
9/29/07 Cascade, MT Mallard Hunter killed Montana Fish, Wildlife & Parks/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
9/27/07 Genesee, NY Mallard Live birds New York State Department of Environmental Conservation/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI N1 virus isolated; H-type unidentifiable (H5 negative) Not applicable
8/27/07 Addison, VT Mallard (#1) Live birds USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
8/27/07 Addison, VT Mallard (#2) Live birds USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
8/27/07 Addison, VT Mallard (#3) Live birds USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
8/27/07 Addison, VT Mallard (#4) Live birds USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
8/27/07 Addison, VT Mallard (#5) Live birds USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
6/18/07 Kent, MD Mallard Live birds USDA/Avian Influenza Coordinated Agricultural Project (AICAP) Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
5/22/07 Kent, DE Ruddy turnstone Live birds USDA/DE Department of Natural Resources and Environmental Control Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
5/21/07 Cape May, NJ Ruddy turnstone Live birds Southeastern Cooperative Wildlife Disease Study Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
5/16/07 Sussex, DE Ruddy turnstone (#1) Live birds USDA/DE Department of Natural Resources and Environmental Control Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
5/16/07 Sussex, DE Ruddy turnstone (#2) Live birds USDA/DE Department of Natural Resources and Environmental Control Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
5/16/07 Sussex, DE Ruddy turnstone (#3) Live birds USDA/DE Department of Natural Resources and Environmental Control Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
5/11/07 Kent, DE Ruddy turnstone Live birds USDA/DE Department of Natural Resources and Environmental Control Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
5/10/07 Cape May, NJ Ruddy Turnstone Live birds Southeastern Cooperative Wildlife Disease Study Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
12/19/06 Hyde, NC American black duck Live birds Southeastern Cooperative Wildlife Disease Study Yes Yes Not related to HPAI H5N1; Suspected LPAI H4N1 Not applicable
11/4/06 Stoddard, MO Northern Shoveler Hunter killed Missouri Conservation Department Yes Yes Not related to HPAI H5N1; Suspected LPAI H1N1 Not applicable
11/4/06 Stanley, SD Northern Shoveler Hunter killed South Dakota Department of Game, Fish and Parks/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
10/28/06 Grundy, IL Mallard ducks Hunter killed IL Dept of Natural Resources/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
10/28/06 Grundy, IL Mallard ducks Hunter killed IL Dept of Natural Resources/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
10/27/06 Sussex, DE Green-winged teal Hunter killed USDA/DE Department of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
10/21/06 Niagara, NY Mallard ducks Hunter killed New York State Department of Environmental Conservation/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
10/21/06 Niagara, NY Mallard ducks Hunter killed New York State Department of Environmental Conservation/USDA Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
10/21/06 Grundy, IL Mallard ducks Hunter killed USDA/IL Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
10/19/06 St. Claire, MI Mallard ducks Hunter killed USDA/MI Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
10/15/06 Tuscola, MI Green-winged teal Hunter killed USDA/MI Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
10/8/06 Ottawa, OH Northern pintail ducks Hunter killed USDA/OH Division of Wildlife Yes Yes Not related to HPAI H5N1; Suspected LPAI No virus isolated Not applicable
9/24/06 Fulton, IL Green-winged teal Hunter killed USDA/IL Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI H6N2 Not applicable
9/21/06 Cascade, MT Northern pintail ducks Live birds USDA/MT Dept of Fish, Wildlife, and Parks Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N3 LPAI
8/28/06 Crawford, PA Mallard ducks Live birds USDA/PA Game Commission Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
8/8/06 Monroe, MI Mute Swans Hunter killed USDA/MI Dept of Natural Resources Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
8/2/06 Queen Annes, MD Resident wild Mallard ducks Environmental USDA/Avian Influenza Coordinated Agricultural Project (AICAP) Yes Yes Not related to HPAI H5N1; Suspected LPAI H5N1 LPAI
1 Testing for the presence of avian influenza (AI) requires that samples be taken from live birds, dead birds or the environment birds inhabit. The Departments of Agriculture and Interior are working collaboratively with States and academic institutions to sample wild birds throughout the United States for the presence of highly pathogenic avian influenza. The interagency plan outlines five specific strategies for early detection of the virus in wild migratory birds, including: investigation of disease-outbreak events in wild birds; expanded monitoring of apparently healthy live wild birds; monitoring of hunter-killed birds; use of sentinel animals; and environmental sampling of bird feces.

2 A series of AI rapid screening tests are performed that cannot differentiate between highly pathogenic AI (HPAI) and low pathogenic AI (LPAI) viruses. The initial rapid screening tests are highly sensitive and can detect active and inactive viruses in samples. Because these rapid screening tests are highly sensitive, it is not uncommon to have positive results for a specific subtype on the initial screen test and yet not be able to isolate a virus of that subtype.

3 This test involves identifying the genetic sequence or key parts of the sequence, of the virus grown and comparing it to known AI genetic sequences. These known sequences, such as that of the highly pathogenic H5N1 AI virus circulating overseas, are stored in databanks. If the genetic sequence matches that of a known highly pathogenic AI virus, the sample is considered to be highly pathogenic.

4 Virus isolation is the gold standard test used to diagnose AI virus infections. The virus is grown and isolated in chicken embryos (eggs). A series of tests follow to specifically identify H and N subtypes of the AI virus. These tests cannot determine pathogenicity.

5 This test involves the inoculation of 4- to 8-week old disease-free chickens and observation for signs of AI for 10 days. According to the USDA and World Organization for Animal Health (OIE), highly pathogenic AI is defined as any AI virus that is lethal for 6 or more of 8 chickens (75% mortality). Pathogenicity is confirmed 10 days from when the chickens are inoculated with the virus grown from the gold standard virus isolation test. Only H5 and H7 subtypes undergo chicken pathogenicity testing because of their potential to become HPAI, all others are considered to be LPAI.

For additional information about Avian Influenza Testing and Diagnostics and Avian Influenza Low Pathogenic H5N1 vs. Highly Pathogenic H5N1, go to www.usda.gov/birdflu and click on fact sheets.

This table is a cooperative effort of the U.S. Departments of Agriculture and Interior, and is managed as part of the National HPAI Early Detection Data System (HEDDS) by the NBII Wildlife Disease Information Node.