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Johnson, H.E., M. Hegsted, and W.J. Blackmon. 1990. Protein quality evaluation of Apios americana tubers. p. 443. In: J. Janick and J.E. Simon (eds.), Advances in new crops. Timber Press, Portland, OR.
Protein Quality Evaluation of Apios americana Tubers
Holly E. Johnson, Maren Hegsted, and William J. Blackmon
Apios americana Medikus (apios) is a nitrogen-fixing legume, which
produces both seeds and tubers. The tubers contain a crude protein content of
12-13% on a dry wt basis. Methionine, cysteine, and isoleuceine are the
limiting amino acids, but some of the cysteine could have been lost in the
hydrolysis reaction of the assay procedure. Metabolic feeding studies with
rats indicate apparent protein digestibility of 81.2±2.4 for 100% boiled
apios (BA) and 42.3±3.4 for a 50:50 mixture of casein:raw apios (C/RA).
Protein digestibility of RA alone could not be determined due to poor food
consumption and weight loss by the rats. Apparent biological value was
89.2±9.8 for the 100% BA, and -81±13.1 for the 50:50 C/RA. Feed efficiency
for the 100% BA was .59±.73 and -16±1.6 for 50:50 C/RA diets. Pancreatic
hypertrophy of the rats consuming the RA indicates a high level of protease
inhibitors. Cholesterol levels were decreased in animals consuming RA,
however, hemoglobin and hematocrit levels were not affected by the presence of
apios in the diets. Cooked tubers consistently performed better than raw
tubers, suggesting the necessity of cooking tubers for use as a food source.
Wilson, P.W., F.J. Pichardo, W.J. Blackmon, and B.D. Reynolds. 1990. Protein
quality in Apios americana tubers and seeds. p. 443. In: J. Janick and
J.E. Simon (eds.), Advances in new crops. Timber Press, Portland, OR.
Protein Quality in Apios americana Tubers and Seeds
P.W. Wilson, F.J. Pichardo, W.J. Blackmon, and B.D. Reynolds
The proteins in Apios americana were studied in terms of total nitrogen,
true protein nitrogen, biuret protein, total amino acids, and in vitro
digestibility. Seeds contained an average of 4.83% total nitrogen on a dry
defatted basis, which would indicate 30.2% crude protein (N x 6.25). However,
the non-protein nitrogen after precipitation with trichloroacetic acid was
1.18%, indicating less "true" protein and only 25.17% protein was found using
the biuret method. Using ion-exchange chromatography, the predominant amino
acid was found to be glutamic acid (79.67 g/100 g N) followed by aspartic acid
(56.97 g/100 g N). The percent of in vitro digestibility for seed protein was
72.2. The computed protein efficiency ratio (C-PER) of A. americana
seeds was 0.99.
Tubers contained an average of 2.12% total nitrogen on a dry defatted basis
(indicating 13.25% crude protein) with 0.48% as non-protein nitrogen. The
biuret protein content of tubers was found to be 13.99%. The predominant amino
acid in tubers was aspartic acid (79.54 g/100 g N) followed by glutamic acid
(47.48 g/100 g N). In vitro digestibility of the tubers was 69.9%.
Picha, D.H., W.J. Blackmon, P.W. Wilson, L.P. Hanson, and B.D. Reynolds. 1990.
Compositional changes in Apios americana tubers during storage. p. 443.
In: J. Janick and J.E. Simon (eds.), Advances in new crops. Timber Press,
Portland, OR.
Compositional Changes in Apios americana Tubers During Storage
D.H. Picha, W.J. Blackmon, P.W. Wilson, L.P. Hanson, and B.D. Reynolds
The tubers of the perennial legume Apios americana Medikus, line
LA85-034, were monitored for changes in dry matter, alcohol-insoluble solids
(AIS) and ethanol soluble sugar under different storage regimes. Tubers left
in the ground after frost-kill of the above-ground vine exhibited little or no
compositional changes when harvested at monthly intervals from December to
March. Tuber dry matter, AIS, and sucrose concentration decreased during April
and May, which corresponded with sprout emergence and growth. Non-sprouted
tubers were high in dry matter (47-50%), high in AIS (39-42%), and low in
fructose, glucose, and maltose (<0.10% fresh wt. of each sugar). Only a
slight loss in dry matter and AIS occurred in tubers stored over a 15 week
period at 4°C or 16°C. Sucrose, the major sugar, increased in
concentration from 1.4% to 2.0% during the first 5 weeks of storage at 16°C,
after which it remained stable over the next 10 weeks. Sucrose synthesis was
enhanced at 4°C storage, with a maximum of 3.2% accumulating after 10 weeks.
No noticeable symptoms of chilling injury existed after 15 weeks storage at
4d°C.
Putnam, D.H., L. Field, and G.H. Heichel. 1990. Inoculation, nitrogen, and
cultivar effects on nodulation and tuber yield of Apios americana. p.
444. In: J. Janick and J.E. Simon (eds.), Advances in new crops. Timber
Press, Portland, OR.
Inoculation, Nitrogen, and Cultivar Effects on Nodulation and Tuber Yield of
Apios americana
D.H. Putnam, L. Field, and G.H. Heichel
Apios americana Medikus (groundnut) is an edible wild tuberous legume
native to the eastern half of North America, and has been proposed as an
alternative specially tuber crop. Accessions of Apios were grown in the
greenhouse and field to assess nodulation, N-requirement, tuber yield and
quality. Tuber dry weight was doubled with the addition of 100 ppm nitrogen in
frequent watering or with inoculation with a common soybean rhizobial strain.
Nodule weight and number were increased significantly by inoculation.
Greenhouse and field tuber yields differed according to accession. Most
accessions overwintered in Minnesota and produced harvestable yields only in
the second year. Significant biological impediments remain to the production
of Apios in this environment. These are viney growth habit, lack of
effective weed control lack of well adapted cultivars, insect problems, and low
yields.
Wells, D.W., R.J. Constantin, W.J. Blackmon, and B.D. Reynolds. 1990.
Evaluations of preemergence herbicides for use in Apios americana. p.
444. In: J. Janick and J.E. Simon (eds.), Advances in new crops. Timber
Press, Portland, OR.
Evaluations of Preemergence Herbicides for Use in Apios americana
D. Wayne Wells, R.J. Constantin, William J. Blackmon, and Berthal D. Reynolds
Selected preemergence herbicides were tested for use in the culture of Apios americana (apios) planted as seeds, transplants in peat pellets, and whole tubers. Herbicide applications were made to transplanted plots prior to planting and to seeded and whole tuber plots after planting. The soil type was a Cahaba fine sandy loam with 0.45% organic matter and 5.5 pH. Apios tolerance to herbicides was associated with planting methods. When planted as seeds, the emerging seedlings appeared to be very prone to herbicidal injury, however, seedling diseases and insect damage prevented definitive evaluations. Mean injury ratings for transplant and tuber plots five weeks after treatment were as follows:
| | Percent injury |
Treatment | Rate kg ai/ha | transplants | tubers |
alachlor | 2.24 | 14 | 3 |
benefin | 1.12 | 0 | 0 |
bensulide | 5.60 | 0 | 0 |
cinmethylin | 2.24 | 14 | 4 |
diethatyl ethyl | 3.36 | 4 | 4 |
ethiozin | 1.68 | 100 | 0 |
linuron | 1.68 | 30 | 3 |
metolachlor | 1.68 | 3 | 5 |
napropamide | 4.48 | 1 | 28 |
oryzalin | 0.84 | 0 | 3 |
trifluralin | 0.84 | 0 | 4 |
bensulide | 4.48 |
" +clomazone | 1.22 | 60 | 1 |
The major weed species were crabgrass (Digitaria spp.), goosegrass
(Eleusine indica), broadleaf signalgrass (Brachiaria
platyphylla), corn spurry (Spergula arvensis), purslane
(Portulaca oleracea), pigweed (Amaranthus
spinosus), and annual sedge (Cyperus iria).
Weed control was only poor to fair with benefin, bensulide, napropamide, and
oryzalin treatments. Other treatments exhibited good to excellent control for
at least some of the weed species present. Reduced weed control was observed
when apios was established from transplants, probably as a result of soil
disturbance after herbicide applications.
Musgrave, M.E., A.G. Hopkins, Jr., and W.J. Blackmon. 1990. Evaluating
Apios americana as a wetland tuber crop. p. 445. In: J. Janick and
J.E. Simon (eds.), Advances in new crops. Timber Press, Portland, OR.
Evaluating Apios americana as a Wetland Tuber Crop
Mary E. Musgrave, Alston G. Hopkins, Jr., and William J. Blackmon
Apios americana Medikus (apios) is a tuber-producing, viny legume whose
natural habitat is characterized by waterlogged soils; however, domestication
efforts have focused on evaluating germplasm on well-drained sites. Some
evidence suggests that tolerance to low oxygen imposed by waterlogging is
associated with the differential activity of respiratory pathways. Among many
cultivated tuber and root crops, the respiration in freshly-sliced storage
tissue is generally cyanide-sensitive but acquires a cyanide-resistant
component as the tissue slices age. Two lines, LA85-034 and LA85-006 were
selected to evaluate respiration patterns. Apios produces tubers on strings
along its underground stem (rhizome). LA85-034 which produces large,
single-node tubers exhibited respiration similar to that typical of Irish
potato and many other storage crops. In contrast, LA85-006 manifested a large
component of cyanide-resistant respiration in freshly sliced tubers. This line
produces small multinode tubers. Rhizome respiration in both lines was highly
cyanide-resistant.
Since cyanide-resistant respiration has been related to waterlogging tolerance,
these two lines are being compared in their response to waterlogging. Tubers
of LA85-006 grew well (82% sprouted) underwater while those of line LA85-034
did not sprout (6%) and would not continue to grow even if presprouted prior to
submergence. These two lines were established in the field and are being
evaluated in their response to longterm waterlogging. Laboratory tests suggest
that there may be considerable variation within apios germplasm with regard to
waterlogging tolerance and that initial selection schemes could influence its
success as a potential wetland tuber crop. Conventional crop plants are unable
to tuberize under anoxic conditions so the ability of apios to tuberize in
waterlogged sites is of interest.
Venketeswaran, S., M.A.D.L. Dias, and U.V. Weyers. 1990. The winged bean: A
potential protein crop. p. 445. In: J. Janick and J.E. Simon (eds.), Advances
in new crops. Timber Press, Portland, OR.
The Winged Bean: A Potential Protein Crop
S. Venketeswaran, M.A.D.L. Dias, and Ursula V. Weyers
The winged bean, Psophocarpus tetragonolobus (L) D.C. is a member of the
Legume family and is a tropical plant found chiefly in rural areas of Papua,
New Guinea and Southeast Asia. It grows abundantly in hot, humid equatorial
countries such as Indonesia, Malaysia, Thailand, Philippines, India,
Bangladesh, Burma and Sri Lanka. Although at one time considered as a "poor
man's food", the potential economic importance of the plant has attracted
world-wide attention and is now recognized as "A High Protein Crop for the
Topics".
The winged bean plant grows as a vine with climbing stems and leaves, 3-4 m in
high. It is a herbaceous perennial; but can be grown generally as an annual.
Although there are several cultivars with wide differences in physical features
and in physiology, the plant produces an abundance of leaves and inflorescences
of white, blue, deep purple or pink flowers which quickly develop into pods.
The pods are 4-sided with fringed wings and can be 6-30 cm in length and
consisting of 5-20 seeds per pod. The seeds which are rich in protein are
comparable to soybean in composition and nutritional value and contain similar
proportions of protein (30-40%), carbohydrates, oil (15-20%), minerals,
vitamins, essential amino acids and other constituents. Besides the various
economical and industrial uses of seeds for commercial exploitation, they are
also useful as food when steamed, boiled, fried, roasted, fermented, made into
milk and other methods. The plant also produces underground tubers of varying
sizes, rich in carbohydrates (25-30%) and proteins (10-15%). The plant is one
of the best nitrogen fixers with nodulation accomplished by soil bacterium,
Rhizobium. Because of its ability to fix nitrogen from the atmosphere,
the plant requires very little or no fertilizers.
Callus tissue and suspension cultures of the winged bean can be established in
a salt-sucrose culture medium supplemented with various combinations of auxins,
viz., 2, 4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA) +
kinetin and/or 6-benzylaminopurine (BA). Regeneration of plantlets can be
achieved via organogenesis or somatic embryogenesis.
Rhoden, E.G., C.K.Bonsi, and M.L. Ngoyi. 1990. Effect of southern root knot
nematode on yield components of yardlong beans. p. 445. In: J. Janick and
J.E. Simon (eds.), Advances in new crops. Timber Press, Portland, OR.
Effect of Southern Root Knot Nematode on Yield Components of Yardlong Beans
E.G. Rhoden, C.K.Bonsi, and M.L. Ngoyi
The influence of different levels of root knot nematode populations on yield
and yield components of yardlong beans [Vigna sesquipedalis (L.) Fruw.]
under greenhouse conditions was investigated. Plants were inoculated with 0,
5,000 or 7,500 larvae of Meloidogyne incognita per 500 ml of soil. Root
knot nematodes reduced seed yield. Plants not inoculated produced 15.97 grams
of seeds while those grown in 5,000 or 7,500 larvae per 500 ml soil produced
6.39 and 6.75 grams per plant, respectively. Inoculated plants flowered later,
had lower shellout percent and less seeds per pod than the controls. The
control plants had more pods per plant (6.80 vs. 4.60 and 4.25) than the
inoculated plants. Nematode inoculation also caused a significant decrease in
the total pod yield of yardlong beans. Plants grown without nematodes produced
22.31 grams while those inoculated with 5,000 or 7,500 larvae per 500 ml soil
produced 9.20 and 9.66 grams of pod per plant respectively
Rhoden, E.G., C.K. Bonsi, and M.L. Ngoyi. 1990. Susceptibility of yardlong
beans to root knot nematode infestation. p. 445. In: J. Janick and J.E. Simon
(eds.), Advances in new crops. Timber Press, Portland, OR.
Susceptibility of Yardlong Beans to Root Knot Nematode Infestation
E.G. Rhoden, C.K. Bonsi, and M.L. Ngoyi
Yardlong bean [Vigna sesquipedalis (L.) Fruw] plants grown in the
greenhouse were inoculated with Meloiidogyne incognita inoculum at the
rates of 0, 5,000 and 7,500 larvae per 500 ml of soil. Increase in nematode
population resulted in a significant increase in the number of eggs per gram of
root fresh weight. Plants inoculated with 7,500 larvae per 500 ml of soil
tended to have greater root volume and dry weight. Although control plants
were taller than those inoculated with M. incognita at anthesis, these
differences were not significant. Plants inoculated with either the low or
high levels of nematode had significantly higher gall and egg mass indices.
Plant dry weight was significantly reduced by nematode infestation. Yardlong
beans appeared to be susceptible to M. incognita.
Last update March 18, 1997
by aw