CRIS Project Title:
Genetic engineering of floral bulb crops for virus and nematode resistance
INTRODUCTION:
Floral bulb crops are propagated vegetatively making it difficult to eliminate
viruses that cause stunting of the plant and streaking on the flowers that
make the flowers unmarketable. Virus resistance by genetic engineering
has been achieved in a variety of crops but not the flower bulb crops. Cucumber
mosaic virus is one of the main viruses in gladiolus and infects numerous agricultural
crops as well. Both gladiolus and lilies will be developed that contain
an antiviral gene for cucumber mosaic virus, and these plants will be analyzed
for their resistance to the virus.
The root lesion nematode is the number one pest of Easter lilies grown in the
field, and it ranks third for the damage that it causes to agricultural crops. Genes
that affect the development of the nematode will be isolated and used to develop
Easter lilies with these genes for possible resistance against nematodes.
PROGRESS:
1. Developed the technology for genetic engineering of gladiolus that
is reproducible and applicable to many cultivars. Applied the technology
to develop gladiolus plants with antiviral genes and found that the plants
had short-term, but not long-term, resistance to bean yellow mosaic virus. 2. Have
developed a system for genetic engineering of Easter lilies that is currently
being optimized. 3. Isolated a polyubiquitin promoter from gladiolus
that has been found to direct high levels of gene expression in gladiolus. This
promoter will be useful for genetic engineering when high levels of a gene
are required for effective resistance.
PLANS:
1. Develop both gladiolus and lilies with a double stranded RNA
for cucumber mosaic virus resistance. 2. Identify genes
that affect the development of the root lesion nematode and use these genes
in lilies to develop resistance to nematodes.
RECENT PUBLICATIONS:
1994. Kamo, K. Effect of phytohormones on plant regeneration from callus of Gladiolus cultivar
`Jenny Lee.' In Vitro Dev. Biol. 30:26-31.
1994. Hammond, J. and Kamo, K. K. Potyvirus resistance derived from antisense
RNA and native or chimeric coat protein genes. Acta Hort. 377:259-266.
1995. Hammond, J. and Kamo, K. K. Resistance to bean yellow mosaic virus (BYMV)
and other potyviruses in transgenic plants expressing BYMV antisense RNA, coat
protein, or chimeric coat proteins. Proceedings of the Fifth International Symposium
on Biotechnology and Plant Protection. College Park, Maryland.
1995. Hammond, J. and Kamo, K. Effective resistance to potyvirus infection conferred
by expression of antisense RNA in transgenic plants. Mol. Plant-Microbe Interact.
8:674-682.
1995. Kamo, K. A cultivar comparison on plant regeneration from suspension cells,
callus, and cormel slices of Gladiolus. In Vitro Cell. Dev. Biol. 31:113-115.
1995. Kamo, K., Blowers, A., Smith, F., Van Eck, J. and Lawson, R. Stable transformation
of Gladiolus using suspension cells and callus. J. Amer. Soc. Hort. Sci.
120:347-352.
1995. Kamo, K., Blowers, A., Smith, F. and Van Eck, J. Stable transformation
of Gladiolus by particle gun bombardment of cormels. Plant Sci., 110:105-111.
1996. Kamo, K., Blowers, A., Smith, F., and Van Eck, J. Genetic transformation
of Gladiolus. In: Biotechnology in Agriculture and Forestry, Vol.38, Springer-Verlag,
pp.222-232.
1996. Kamo, K. Bean yellow mosaic virus coat protein and gusA gene
expression in transgenic Gladiolus plants. Acta Hort. 447: 393-401.
1996. Griesbach, R.J. and Kamo, K.K. The effect of induced polyploidy
on the flavonols of Petunia 'Mitchell.' Phytochemistry 42:361-363.
1997. Kamo, K. Factors affecting Agrobacterium tumefaciens-mediated gusA expression
and opine synthesis in Gladiolus. Plant Cell Reports 16:389-392.
1997. Kamo, K. and Van Eck, J. Effect of bialaphos and phosphinothricin
on plant regeneration from long- and short-term callus cultures of Gladiolus. In
Vitro Cell. Dev. Biol.-Plant 33:180-183.
1997. Kamo, K. Hammond, J. and Roh, M. Transformation of Gladiolus for
disease resistance. Journal of the Korean Society for Horticultural Science. 38:188-193.
1999. Kamo, K. and Blowers, A. Tissue-specific and levels of gusA expression
under either the rolD, mannopine synthase, or translation elongation factor
1 subunit α promoters in transgenic Gladiolus plants. Plant
Cell Reports 18:809-815.
2000. Kamo, K., Blowers, A., and McElroy, D. Effect of the cauliflower
mosaic virus 35S, actin, and ubiquitin promoters on uidA expression from
a bar-uidA fusion gene in transgenic Gladiolus plants. In Vitro
Cell. Dev. Biol.-Plant 36:13-20.
2000. Kamo, K., McElroy, D., and Chamberlain, D. Transforming embryogenic
cells lines of Gladiolus with either a bar-uidA fusion gene or
cobombardment. In Vitro Cell. Dev. Biol.-Plant 36:182-187.
2000. De Villiers, S. M., Kamo, K., Thomson, J. A., Bornman, C. H. and
Berger, D. K. Biolistic transformation of chincherinchee (Ornithogalum)
and regeneration of transgenic plants. Physiol. Plant. 109: 450-455.
2001. Kamo, K., Roh, M., Blowers, A., Smith, F. and Van Eck, J. Transgenic Gladiolus. In: Y.P.S. Bajaj (Editor) Biotechnology and Agriculture and Forestry 48. Transgenic Crops III, Springer Verlag, Berlin, pp. 155-169.
2001. Joung, Y. H., Roh, M. S., Kamo, K. and Song, J. S. Agrobacterium-mediated transformation of Campanula glomerata. Plant Cell Rep. 20:289-295.
2001. Kamo, K. Expression of the bar and uidA genes by Gladiolus following three seasons of dormancy. Acta Hort. 560:165-167.
2002. Castillon, J. and Kamo, K. Maturation and conversion of somatic embryos of three genetically diverse rose cultivars. HortScience 37:973-977.
2002. Joung, Y. H., Liao, M. S., Roh, M. S., Kamo, K. and Song, J. S. In vitro propagation of Campanula glomerata, ‘Acaulis’ from leaf blades. Scientia Hort. 92:137-146.
2002. Lipsky, A., Cohen, A., Gaba, V., Kamo, K., Gera, A., Watad, A. Transformation of Lilium longiflorum plants for cucumber mosaic virus resistance by particle bombardment. Acta Hort. 568:209-214.
2003. Kamo, K. K. Long-term expression of the uidA gene in Gladiolus plants under control of either the ubiquitin, rolD, mannopine synthase, or cauliflower mosaic virus promoters following three seasons of dormancy. Plant Cell Rep. 21:797-803.
2004. Kamo, K., Jones, B., Castillon, J., Bolar, J. and Smith, F. Dispersal and filtration of embryogenic callus increases the frequency of embryo maturation and conversion for hybrid tea roses. Plant Cell Rep. 22:787-792.
2004. Kim, C. K., Chung, J. D., Park, M. Kamo, K. and Byrne, D. H. Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene. Plant Cell Tiss. Org. Cult. 78:107-111.
2004. Ahn, J., Joung, Y.H., and Kamo, K. Transformation of Easter lilies. Transgenic plants of Easter lily (Lilium longiflorum) with phosphinothricin resistance. J. Plant Biotech. 6:9-13.
2004. Kamo, K., Gera, A., Cohen, J., Hammond, J., Blowers, A., Smith, F. and Van Eck, J. Transgenic Gladiolus plants transformed with the Bean yellow mosaic virus coat protein gene in either sense or antisense orientations. Plant Cell Rep. 23:654-663.
2005. Aebig, J. A., Kamo, K. and Hsu, H.T. Biolistic inoculation of gladiolus with cucumber mosaic cucumovirus. J. Virol. Meth. 123:89-94.
2005. Kamo, K., Jones, B., Bolar, J. and Smith, F. Regeneration from long-term embryogenic callus of the Rosa hybrida cultivar Kardinal. In Vitro Cell. Dev. Biol.-Plant 41:32-36.
|
Research Unit Home Page ||
Mission & Goals ||
History ||
Locations
Scientists & Specialties ||
Postdoctorals & Support Scientists ||
Technical & Support Staff
Recent Accomplishments ||
Publications & Patents ||
New Plant Introductions
Woody Landscape Plants Germplasm Repository ||
National Herbarium
Last Updated October 30, 2006 11:17 AM
URL = http://www.usna.usda.gov/Research/~kkk.html