Southern Research Station

Title: Comparison of RAPD Linkage Maps Constructed For a Single Longleaf Pine From Both Haploid and Diploid Mapping Populations

Author: Kubisiak, Thomas L.; Nelson, C.Dana; Name, W.L.; Stine, M.

Date: 1996

Source: Forest Genetics 3(4):203-211, 1996

Description: Considerable concern has been voiced regarding the reproducibility/transferability of RAPD markers across different genetic backgrounds in genetic mapping experiments. Therefore, separate gametic subsets (mapping populations) were used to construct individual random amplified polymorphic DNA (RAPD) linkage maps for a single longleaf pine (Pinus palustris Mill.). A haploid mapping population consisting of megagametophytic DNAs Tom 88 wind-pollinated seeds of longleafpine (clone 3-356), and a diploid population including 86 F₁ progeny fom a controlled cross [longleaf pine 3-356 (female) × slash pine H-28 (male)] were employed. Seventy-one, RAPD primers selected for this study identified a total of 137 mapped loci in longleaf pine 3-356 based on amplification of megagametophytic DNAs. Of the 137 loci useful for comparative purposes, 62 loci (45.30%) could not be scored when DNAs of F₁ progeny from the controlled cross were amplified. Of the 75 loci that were storable, 26 loci (34.7%) were fixed in slash pine H-28, and 49 (65.3%) were segregating (42 1:1, and seven 3:1). Comparisons were made using the 49 loci common to both the haploid-and diploid-based maps. The 49 loci allowed us to determine homologous counterparts maps. Orders were conserved for those groupscontaining three or more loci. Genetic distance estimates were found vary considerably, but not in anysystematic manner.

Keywords: random amplified polymorphic DNAs (RAPDs), Pinus palustris Mill., genetic linkage maps, megagametophytic DNA

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Citation

Kubisiak, Thomas L.; Nelson, C.Dana; Name, W.L.; Stine, M.  1996.  Comparison of RAPD Linkage Maps Constructed For a Single Longleaf Pine From Both Haploid and Diploid Mapping Populations .   Forest Genetics 3(4):203-211, 1996