Southern Research Station

Title: Polymorphic sequence-characterized codominant loci in the chestnut blight fungus, Cryphonectria parasitica

Author: Davis, J. E.; Kubisiak, Thomas L.; Milgroom, M. G.

Date: 2005

Source: Molecular Ecology Notes (2005) 5, 195-197

Description: Studies on the population biology of the chestnut blight fungus, Cryphonectria parasitica, have previously been carried out with dominant restriction fragment length polymorphism (RFLP) fingerprinting markers. In this study, we described the development of 11 condominant markers from randomly amplified polymorphic DNAs (RAPDs). RAPD fragments were cloned and sequenced, polymerase chain reaction (PCR) primers were designed flanking insertions/deletions. Primers labelled with fluorescent dyes were combined in multiplex reactions to assay five or six loci simultaneously in a capillary sequencing system. These codominant markers have the potential to complement RFLP methods for studying C. parasitica.

Keywords: chestnut blight fungus, Cryphonectria parasitica, RAPD, SCAR, sequence-characterized amplified region

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Citation

Davis, J. E.; Kubisiak, Thomas L.; Milgroom, M. G.  2005.  Polymorphic sequence-characterized codominant loci in the chestnut blight fungus, Cryphonectria parasitica.   Molecular Ecology Notes (2005) 5, 195-197