Using the Genetic Inducible Fate mapping technique based on the inducible Cre recombinase/loxP system, Shh- responding (Gli1-expressing) neural stem cells are permanently marked in Gli1-CreER; Tau-stop-mGFP-IRES-nlacZ mice following Tamoxifen administration. New granule neurons generated from these stem cells in the adult dentate gyrus of the hippocampus can be visualized by their nuclear lacZ (Red) expression and projections (Green) toward CA3 region. (Blue: DAPI nuclear counter-staining).