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| Sanford P. Markey, Ph.D., Senior Investigator |
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Dr. Markey received his A.B. from Bowdoin College in 1964. He received his Ph.D. in Chemistry from the Massachusetts Institute of Technology in 1968, doing structural analysis of complex mixtures of organic compounds using mass spectrometric methods with Klaus Biemann. In a faculty position in Pediatrics and Pharmacology at the University of Colorado Medical Center, he developed and applied gas chromatographic-mass spectrometric-computer methods to the diagnosis of inborn errors of metabolism. Dr. Markey joined NIMH in 1974 and developed a laboratory to investigate the metabolism of small molecules important in brain biochemistry. Dr. Markey's laboratory is studying proteins in sub-cellular structures, particularly those relevant to neuronal development.
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Staff:
- Cai Chen, B.S., Research Assistant caichen@mail.nih.gov
- Dr. Christopher Ellis, Ph.D., Research Fellow, Contractor cellis@nhgri.nih.gov
- Ewa Jankowska-Stephens, Contractor jankowskastephense@mail.nih.gov
- Jeffrey Kowalak, Ph.D., Staff Scientist kowalakj@mail.nih.gov
- Claudine Laurent, M.D., Ph.D., Postdoctoral Fellow laurentc@mail.nih.gov
- Anthony J. Makusky, B.S., Research Assistant makuskya@mail.nih.gov
- Dr. Melinda McFarland, Ph.D., IRTA Fellow mmcfarla@mail.nih.gov
- Dr. Dhaval Nanavati, Ph.D., IRTA Fellow nanavatid@mail.nih.gov
- Elaine Raab, Secretary raabe@mail.nih.gov
- Dr. Douglas Slotta, Ph.D., IRTA Fellow slottad@mail.nih.gov
- Dr. Michael Brad Strader, Ph.D., Research Fellow straderm@mail.nih.gov
Research Interests:
The Laboratory of Neurotoxicology conducts research to determine the biochemical events that cause neuronal injury and degeneration accompanying genetic disease, inflammation, trauma, and exposure to neurotoxic substances.
The Analytical Biochemistry Section emphasizes research that utilizes qualitative and quantitative molecular structure analyses to determine cellular responses to diseases and neurotoxins. Methods for the mass spectrometric analysis of protein structures are being improved, applied and tested in projects in proteomics. Software to aid in these studies is also being developed.
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Selected Recent Publications:
McFarland MA, Ellis CE, Markey SP, Nussbaum RL (2008) Proteomic analysis identifies phosphorylation-dependent a-synuclein protein interactions, Mol Cell Proteomics.
Full Text/Abstract
Savas JN, Makusky A, Ottosen S, Baillat D, Then F, Krainc D, Shiekhattar R, Markey SP, Tanese N (2008) Huntington's disease protein contributes to RNA-mediated gene silencing through association with Argonaute and P bodies, Epub 105(31), 10820-5.
Full Text/Abstract
Lockwich T, Pant J, Makusky A, Jankowska-Stephens E, Kowalak JA, Markey SP, Ambudkar IS (2008) Analysis of TRPC3-interacting proteins by tandem mass spectrometry, J Proteome Res 7(3), 979-89.
Full Text/Abstract
Nanavati D, Gucek M, Milne JL, Subramaniam S, Markey SP (2008) Stoichiometry and absolute quantification of proteins with mass spectrometry using fluorescent and isotope-labeled concatenated peptide standards, Mol Cell Proteomics 7(2), 442-7.
Full Text/Abstract
Harrington PB, Laurent C, Levinson DF, Levitt P, Markey SP (2008) Bootstrap classification and point-based feature selection from age-staged mouse cerebellum tissues of matrix assisted laser desorption/ionization mass spectra using a fuzzy rule-building expert system, Anal Chim Acta 599(2), 219-31.
Full Text/Abstract
Dosemeci A, Makusky AJ, Jankowska-Stephens E, Yang X, Slotta DJ, Markey SP (2008) Composition of the synaptic PSD-95 complex, Mol Cell Proteomics 6(10), 1749-60.
Full Text/Abstract
Fujigaki H, Saito K, Lin F, Fujigaki S, Takahashi K, Martin BM, Chen CY, Masuda J, Kowalak J, Takikawa O, Seishima M, Markey SP (2006) Nitration and inactivation of IDO by peroxynitrite, J Immunol 176(1), 372-9.
Full Text/Abstract
All Selected Publications
Contact Information:
Dr. Sanford P. Markey
Analytical Biochemistry Section
Laboratory of Neurotoxicology, NIMH
Building 10, Room 3D42
10 Center Drive, MSC 1262
Bethesda, MD 20892-1262
Telephone: (301) 496-4022 (office),
(301) 451-5780 (fax)
Email: markeys@mail.nih.gov
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