NCBI
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BLAST 2 sequences
BLAST
Example
Help
BLAST 2 SEQUENCES
This functionality is now available in the main
BLAST
pages and this link will be removed in the near future. Step-by-step
instructions
for use are available.
This tool produces the alignment of two given sequences using
BLAST
engine for local alignment.
The stand-alone executable for blasting two sequences (bl2seq) can be retrieved from
NCBI ftp site
Reference:
Tatiana A. Tatusova, Thomas L. Madden (1999), "Blast 2 sequences - a new tool for comparing protein and nucleotide sequences", FEMS Microbiol Lett. 174:247-250
Program
blastn
blastp
tblastn
blastx
tblastx
Matrix
Not Applicable
Parameters used in
BLASTN
program only:
Reward for a match:
Penalty for a mismatch:
Use
Mega BLAST
Strand option
Both strands
Top strand
Reverse strand
View option
Standard
Mismatch-highlighting
Masking character option
X for protein, n for nucleotide
Lower case
Masking color option
Black
Grey
Red
Show CDS translation
Open gap
and extension gap
penalties
gap x_dropoff
expect
word size
Filter
Sequence 1
Enter accession, GI or sequence in FASTA format
from:
to:
or upload FASTA file
Sequence 2
Enter accession, GI or sequence in FASTA format
from:
to:
or upload FASTA file
Comments and suggestions to
blast-help@ncbi.nlm.nih.gov