Brief description of the proposed target/antigen (full length protein,
specific region or peptide sequence, specific post-translational
modification, etc).
(example: The target is Fibroblast Growth Factor 14.  A fusion protein consisting of amino acids 1-252 (full-length) of mouse FGF14b will be provided.)

Briefly describe the biological importance of the target
protein/antigen.
(example: Humans with an autosomal dominant missense mutation in fibroblast growth factor 14 (FGF14) have impaired cognitive abilities and slowly progressive spinocerebellar ataxia.)

Briefly describe the importance of the proposed antibodies to the
epigenetics research community.
(example: Information about the importance of the Ab to the wider epigenetics community should be put here.

Briefly describe the availability and suitability (or lack thereof) of
existing polyclonal and/or monoclonal antibodies.
(example: None of the existing antibodies (polyclonal) recognize FGF14 specifically.)

What reagents (fusion proteins, full-length cDNAs for transfection in
mammalian cells, samples from knockout mice) could you provide to a
monoclonal antibody production facility to aid in the development and
characterization of these antibodies?  
(example: I can provide a full length fusion protein of the b splice variant, tagged cDNAs for screening in COS cells and samples from knockout mice.)

If fusion proteins cannot be supplied, are you willing to provide advice
on synthetic peptides that could be synthesized? Costs for purchasing
synthetic peptides would be born by the Roadmap Epigenomics Program.  
(example: A fusion protein is being provided.)

Would you be willing to participate in screening a limited number of
hybridoma clones (less than 20) to identify those clones which produce
useful monoclonal antibody?  
(example: Yes. In the actual example, the resulting mouse monoclonal antibody was of the IgG1 isotype, recognized the a and b splice variants of FGF14 from mouse, human and rat, and did not cross-react with FGF11, 12 or 13.)