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Fish ResourcesON THIS PAGE: National Resource for ZebrafishResearch Emphasis/ObjectivesThe objective of the Zebrafish International Resource Center Services ProvidedAnimalsHealthy stocks of zebrafish and frozen sperm are maintained and distributed to the research community. Strains of wild-type fish and lines carrying mutations and transgenes are accepted from the research community, maintained, and distributed on request. Researchers are welcome to visit the resource center to screen the collection for mutations of interest or to learn procedures. Advice about zebrafish health is provided. Diseased fish and tissue samples may be sent to the resource center for pathology analysis. The center also provides routine sentinel or quality control testing of zebrafish from healthy laboratory colonies (e.g., to fulfill IACUC health status monitoring). Biological MaterialsAntibodies, gene probes, and other markers to analyze wild-type and mutant stocks as well as zebrafish cell lines are received, stored, and distributed. Informatics
The center maintains an online database (ZFIN Contact Information
Zebrafish International Resource Center
Web site: zfin.org/zirc Grant No.: P40 RR12546
Principal Investigator
Additional Contact Xiphophorus Genetic Stock CenterResearch Emphasis/Objectives
The
Xiphophorus Genetic Stock Center Current ResearchThe stock center is continuing saturation of the Xiphophorus gene map and development of expressed sequence tag (EST) markers providing investigators with comparative mapping data to approach complex scientific questions. Ongoing efforts involve molecular characterization of physical genetic markers (microsatellite and ESTs) informative for gene mapping and genetic analyses in several commonly used Xiphophorus interspecies crosses. The stock center also is establishing technology that will allow cryopreservation of Xiphophorus sperm samples and thereby provide a preservation program where the XGSC maintains the genetic heritage of all Xiphophorus strains and species. The unique physiology of Xiphophorus sperm has limited previous efforts at freezing of sperm for long-term storage using standard protocols. In collaboration with Dr. Terry Tiersch of Louisiana State University, recent advances in cryopreservation of fish sperm are being studied to develop protocols allowing long-term sperm storage and retrieval of active sperm samples. Services ProvidedSince its establishment more than 70 years ago, the XGSC has provided pedigreed fish from more than 60 genetic strains to scientists and aquarists around the world. Scientists in more than 40 laboratories in the United States, Singapore, Canada, Mexico, Japan, Colombia, and Germany work on Xiphophorus genetics and depend on strains available from the XGSC. Backcross hybrid tissues and nucleic acid panels are being developed and will be available to investigators. Primer pair sequences corresponding to genetic markers and assay information for several interspecies crosses will be made available to the research community. In addition to supplying strains and providing consultation on husbandry and genetic questions, the XGSC produces custom interspecies hybrids (both first generation F1, and backcross hybrid generation BC1) for a variety of projects, shipping hundreds of such fish each year. The XGSC will assist researchers in performing detailed genetic mapping analyses on fish samples where a collaborating researcher has scored a phenotype of interest. Contact Information
Department of Chemistry and Biochemistry
Web site: xiphophorus.org Grant No.: P40 RR017072
Principal Investigator and Director
Additional Contact Ordering InformationSee contact listed above. |
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National Institutes of Health (NIH) Bethesda, Maryland 20892 |
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Department of Health and Human Services |
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