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Rodent ResourcesON THIS PAGE:
Knockout Mouse Project (KOMP) RepositoryThe Knockout Mouse Project (KOMP) Repository was established in May 2007 to archive and distribute the approximately 8500-10,000 KOMP vectors and ES cell clones) produced by the 2 KOMP Mutagenesis Teams at the CSD Consortium (Children's Hospital Oakland Research Institute [CHORI], The Sanger Institute, and UC Davis) and at Regeneron, Inc. The KOMP Repository is located at UC Davis and a subcontract to CHORI. The purpose of the KOMP Repository is to ensure the preservation, protection, availability, and accessibility of KOMP products, resources, and services for use by the scientific community. The repository is built upon the closely linked infrastructure, technical support, and faculty expertise within the UC Davis Mouse Biology Program (MBP), Center for Comparative Medicine, and Schools of Medicine and Veterinary Medicine, and the CHORI-BACPAC Resource. UC Davis shall archive, maintain, and distribute all KOMP ES cell clones, live mouse lines, and frozen embryos and sperm, while CHORI shall archive, maintain, and distribute all KOMP vectors. Research Emphasis/ObjectivesThe objectives of the KOMP Repository are to: 1) conduct QC procedures to verify the viability, identity, and utility of all KOMP products; 2) enable fast, simple, and easy online searching and ordering for any KOMP product or service (e.g., microinjection, ICSI for frozen B6 sperm, pathology analysis, etc); 3) distribute KOMP products promptly and for reasonable cost, and 4) provide responsive and attentive customer and technical service. These objectives will be accomplished by expanding, genotyping, karyotyping, pathogen-screening, and reanimating ES cell clones generated by the KOMP Mutagenesis Teams and Regeneron, Inc in preparation for distribution to the research community. Services Provided
In addition to obtaining KOMP products (vectors and ES cell clones), investigators will have full access to all UC Davis resources and services, including reanimation (i.e., microinjection) of ES cells into live mice, conversion of ES cells and live mice to cryopreserved embryos and sperm, and conversion of a cryopreserved archive into live mice. The KOMP repository shall also maintain an easily-navigable and informative public Web site (www.komp.org Contact Information
The Knockout Mouse Project
Web site: www.komp.org Grant No.: U42 RR024244
Principal Investigator and Director
Pieter J. de Jong, Ph.D.
Additional Contact: KOMP Manager Mutant Mouse Regional Resource CentersThe Mutant Mouse Regional Resources Centers (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. The MMRRCs accept, cryopreserve, maintain, and distribute mutant mouse strains for use in biomedical research. The centers take transgenics, knockouts, and all other kinds of induced mutant mouse lines at no cost to the donors, and after specific-pathogen-free rederivation and cryopreservation, distribute mice for a small fee to requesting investigators for noncommercial, academic research purposes only.
The MMRRCs currently have a census of more than 10,000 mutant strains
maintained as either actively breeding mouse colonies, frozen embryos
or germplasm, and/or embryonic stem cell clones. Investigators
interested in donating mouse strains or finding out what mice are
available for distribution should visit: www.mmrrc.org Genetically Engineered Strains Now Available: If you are investigating mutant mouse strains for your research projects, please visit the MMRRC Catalog Search Form on the MMRRC Web site. The search form provides links to register interest for strains not yet available for distribution and to the Strain (or cell line) Detail Sheets for available mice (and cell lines). Furthermore, the form includes checkboxes that enable researchers to request available strains, strains in development, or strains awaiting transfer from donating investigators, or any combination of the three. Mutant Mouse Regional Resource Center at the University of Missouri/HarlanThe Mutant Mouse Regional Resource Center (MMRRC) at the University of Missouri/Harlan (MH-MMRRC) was established in 2000. The MH-MMRRC is a multi-institutional consortium of investigators including John K. Critser, Ph.D.; Beth Bauer, D.V.M; and Lela K. Riley, Ph.D., at the University of Missouri and Robert J. Russell, D.V.M., at Harlan Sprague Dawley, Inc. Research Emphasis/ObjectivesResearch projects are focused in the following major areas of importance to mice as animal models: 1) development of efficacious methods for nuclear transfer in the mouse; 2) development of efficient methods for genome resource banking and strain/stock reconstitution, including ovarian tissue preservation and spermatozoa cryopreservation; 3) improved methods for genotyping; and 4) improved methods for health monitoring of mice using molecular diagnostics, environmental monitoring, and biosensors. Services ProvidedThe overall goal is to establish a center for distributing high-quality, well-characterized inbred, hybrid, and mutant mice to investigators. To this end, the MH-MMRRC will select and import mouse strains and stocks important to the biomedical research community; rederive mice to a pathogen-free state; cryopreserve gametes and embryos; perform genotyping, phenotyping, and infectious disease monitoring to ensure the quality of the mice; and distribute mice to investigators as live mice, cryopreserved germplasm, or tissues. Contact Information
University of Missouri
Web site: www.mmrrc.org Grant No.: U42 RR014821
Principal Investigators
Lela K. Riley, Ph.D.
Additional Contacts
Robert Russell, D.V.M. Mutant Mouse Regional Resource Center at University of California, DavisResearch Emphasis/ObjectivesThe overall objective of the UC Davis Mutant Mouse Regional Resource Center is to serve as a component of the MMRRC National Program, to accept, maintain, and distribute mutant mice for use in biomedical research. The MMRRC Program accepts transgenics, knockouts, and all other kinds of mutant mouse lines at no cost to the donor, and after re-derivation and cryopreservation, distributes breeding stock or germplasm of genetically-defined and pathogen-free mice at a small fee to requesting investigators. The UC Davis MMRRC currently has a census of over 10,000 mutant strains that are maintained as live breeding colonies, frozen embryos or germplasm, and embryonic stem cell clones. Furthermore, the MMRRC serves as a center of excellence in mouse biology, mouse genomic manipulation, genetic and genotyping services, strain rescue, pathology and clinical pathology phenotyping, behavioral phenotyping, non-invasive imaging, and infectious disease diagnostics. These services are available to the biomedical research community on a collaborative or fee-for-service basis. The MMRRC provides support for applied research that is aimed at enhancing the resource, and serves as a teaching environment that is linked to residency and graduate training programs in the Center for Comparative Medicine. Current ResearchAreas of applied research that are supported by the MMRRC include evaporative drying of mouse sperm, oocyte and blastocyst cryopreservation, accelerated speed congenics, development of optimized SNPs, multiplex detection of antibody to infectious agents, and vertical transmission of infectious agents through germplasm. Services ProvidedCryopreservation, rederivation, strain rescue, construct design, random and targeted mutations, speed congenics, genotyping, in vitro fertilization, intracytoplasmic sperm injection, pathology and behavioral phenotyping, diagnostics, experimental pathology, non-invasive imaging, data and image analysis, colony management, and teaching resources. Contact Information
Center for Comparative Medicine
Web site: ccm.ucdavis.edu Grant No.: U42 RR14905
Principal Investigator Mutant Mouse Regional Resource Center at University of North CarolinaResearch Emphasis/ObjectivesUNC-Chapel Hill is one of four MMRRC centers that are developing and maintaining a mutant mouse resource for the benefit of the entire biomedical community. To meet demands for MMRRC services and improve upon existing technologies, the goals of the UNC-Chapel Hill Center are to: 1) import, archive and distribute genetically modified mouse strains; 2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community; 3) develop improved and reliable methods for assisted fertilization; 4) integrate gnotobiotics technology into MMRRC services; and 5) generate an allelic series of point mutations in genes of interest as a community resource. Current ResearchStreamline and improve operating procedures to increase importation, cryoarchiving and distribution of mice. Develop improved methods for sperm phenotyping, assisted in vitro fertilization, intracytoplasmic sperm injection. Collaborate with the National Gnotobiotic Rodent Resource Center to provide gnotobiotic strains, technology development, and relevant phenotyping services. Generate an allelic series of point mutations in genes of interest. Services ProvidedMaintain selected strains of genetically modified mice; distribute mutant mice from live colony; distribute mutant mice from cryopreserved archive; health testing; frozen sperm, oocytes, ovaries; embryo, cropreservation and rederivation; sperm phenotyping; intracytoplasmic sperm injection; assisted IVF; ES cell library screening for ENU induced point mutations; ES cell microinjection; genotyping; and histopathology. Contact Information
MMRRC-Chapel Hill Grant No.: U42 RR014817
Principal Investigator
Additional Contact Informatics, Coordination and Service Center for the Mutant Mouse Regional Resource CentersResearch Emphasis/ObjectivesThe MMRRC-ICSC provides a centralized informatics resource and customer service center for the three MMRRC mutant mouse resource centers described above. The ICSC maintains the MMRRC public and internal web sites, maintains the database that supports the MMRRC catalog and web site, provides software support for MMRRC programmatic projects, such as order tracking and customer surveys, provides administrative support for MMRRC monthly meetings and strain review and serves as the customer service center for all mouse strains and ES cell lines held by the MMRRC. Although the ICSC does not carry out research per se, it is responsible for upgrading and enhancing software and database management systems that support the informatics and customer service activities. Services Provided
The ICSC maintains a web site with information on the MMRRC and mouse resources and services available and provides a single contact point for scientists to request mouse strains or ES cell lines held in the three MMRRC resource centers. Mice or ES cells can be ordered by calling the MMRRC-ICSC Customer Service Center at 1-800-910-2291 (North America) or 207-288-6009 (international), by emailing: service@mmrrc.org, or by faxing 207-288-6150. Access the searchable database and online Submission and Order Request Forms at http://www.mmrrc.org/ Contact Information
MMRRC-ICSC
Web site: http://www.mmrrc.org Grant No.: N02 RR62090
Principal Investigator and Contact
Additional Contacts Induced Mutant ResourceResearch Emphasis/ObjectivesResearch is being conducted on improved methods for assisted reproduction and speed congenic production. Most of the targeted mutants arrive on a mixed 129xC57BL/6 genetic background, and as many of these as possible are backcrossed onto an inbred strain (usually C57BL/6J). In addition, new mouse models are being created by intercrossing carriers of specific transgenes and/or targeted mutations. Simple sequence length polymorphism DNA markers are being used to characterize and evaluate differences between inbred strains, substrains, and embryonic stem cell lines. Services ProvidedThe Induced Mutant Resource (IMR) at The Jackson Laboratory was established in September 1992 in response to concerns from the scientific community regarding the cost, health, and distribution of genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research.
More than 1000 mutant stocks have been accepted by the IMR from 1992
through December 2006. Current holdings include models for research on
cancer; breast cancer; immunological and inflammatory diseases;
neurological diseases; behavioral, cardiovascular and heart diseases;
developmental, metabolic and other diseases; reporter (e.g., GFP) and
recombinase (e.g., cre/loxP) strains. About eight strains a month are
being added to the IMR holdings. A list of all strains can be obtained
from the IMR Web site
All mice can be ordered by calling The Jackson Laboratory's
Customer Service Department at 1-800-422-MICE or 207-288-5845 or by
faxing 207-288-6150. Access the searchable database
For more information about the resource, contact any of the four
investigators listed below or The Jackson Laboratory's Technical
Information Service at micetech@jax.org. Updates on strain
availablility and repository information are accessible at jaxmice.jax.org/findmice/repository.html Contact Information
The Jackson Laboratory
Web site: www.jax.org/imr Grant No.: P40 RR009781
Principal Investigator and Contact
Additional Contacts
Leah Rae Donahue, Ph.D.
Cathleen M. Lutz, Ph.D. Mouse Mutant Gene ResourceResearch Emphasis/ObjectivesResearch objectives include the following: to discover and characterize new mouse models of human inherited conditions; to maintain as breeding pairs and preserve as frozen embryos new and established mouse mutations and chromosomal aberrations; to develop genetically suitable stocks of new and established mouse mutations for use in biomedical research; and to make these mutant stocks available to interested investigators in the scientific community. Services Provided
This resource encourages collaborations with visiting investigators to
screen mutant and wild-derived strains for specific conditions,
symptoms, biochemical or physiological defects, behavior, or other
phenotypes of interest. The resource provides technical support for
users of JAX mice to answer questions regarding genetics, husbandry,
and characteristics of mutant mice. All mice can be ordered by calling
The Jackson Laboratory's Customer Service Department at
1-800-422-MICE or 207-288-5845 or by faxing 207-288-6150. Access the
searchable database
A Strain Submission Form
Available Mouse Strains
This resource develops and maintains strains of mice with specific
mutant genes in various categories, including growth and development,
reproduction, neurological, neuromuscular, vision and hearing,
skeletal, immunological, skin and hair, pigmentation, kidney, and
enzyme deficiencies. It also maintains stocks of mice with chromosomal
aberrations including inversions, translocations, monosomy, and
trisomy. In addition, several wild-derived inbred strains are
maintained for linkage crosses. Updates on strain
availablility and repository information are accessible at jaxmice.jax.org/findmice/repository.html Contact Information
The Jackson Laboratory
Web site: www.jax.org/mmr/index.html Grant No.: P40 RR001183
Principal Investigator
Additional Contacts
Leah Rae Donahue, Ph.D.
Eva M. Eicher, Ph.D. National Gnotobiotic Rodent Resource CenterResearch Emphasis/ObjectivesThis resource allows NIH-funded investigators to explore genetic/environmental interactions. Gnotobiotic mice and rats are used to investigate the physiologic and pathophysiologic consequences of colonizing normal and genetically mutated hosts with commensal bacterial flora or with individual components of this complex ecosystem. Contact Information
University of North Carolina-Chapel Hill Grant No: P40 RR018603
Principal Investigator Peromyscus Genetic Stock CenterResearch Emphasis/ObjectivesDevelopment of cryopreservation techniques that are applicable to Peromyscus sperm to enable long-term preservation of mutant stocks. The mechanism and genetics of hybrid dysgenesis in reciprocal hybrids of P. maniculatus and P. polionotus. Offspring of matings in one direction are markedly oversized; the reciprocal cross produces undersized offspring. Development of an intermediate density map utilizing PCR-based type 1 (functional genes) and type II (microsatellite) markers as well as single gene mutants with coat color or neurological phenotypes; and interspecific crosses between P. maniculatus and P. polionotus as well as whole genome radiation hybrids for linkage determinations. Fruition of the mapping project will benefit a wide range of investigators interested in using Peromyscus to study the genetic basis of speciation, genome imprinting, disease vector biology, and behavioral and physiological adaptation to habitat. Services Provided
The Peromyscus Genetic Stock Center was established in 1985 and
functions to provide a reliable source of genetically defined and
virus-free animals and related materials to the scientific and
educational communities. The center currently keeps nine species and
more than 27 distinctive mutant and other genetically defined stocks.
Included among the species maintained are P. californicus, P.
leucopus, P. eremicus, P. aztecus, P. melanophrys as well as two
subspecies each of P. maniculatus and P. polionotus and
three inbred lines of P. leucopus. Among the mutant stocks are
17 with altered coat phenotypes, 3 with neurological symptoms, and 6
others with developmental/physiological effects. One of these is a
line deficient in alcohol dehydrogenase that has been widely used in
studies of alcohol metabolism. The stock center also supplies
biological materials, including fresh, frozen, and preserved tissues;
molecular probes, and libraries. The center functions as a
clearinghouse for information regarding this genus by sponsoring an
Internet database (i.e., PeroBase Contact Information
University of South Carolina
Web site: stkctr.biol.sc.edu Grant No.: P40 RR014279
Principal Investigator
Co-investigator
Contact and Colony Manager Rat Resource and Research CenterThe Rat Resource and Research Center was established in 2001. The center is a multi-institutional consortium of investigators, including John K. Critser, Ph.D.; Beth Bauer, D.V.M.; and Lela K. Riley, Ph.D., at the University of Missouri. Research Emphasis/ObjectivesResearch projects are focused in the following major areas of importance to rats as animal models: 1) development of efficacious methods for nuclear transfer in the rat; 2) development of efficient methods for genome resource banking and strain/stock reconstitution, including ovarian tissue preservation and spermatozoa cryopreservation; 3) improved methods for genotyping; and 4) improved methods for health monitoring of rats using molecular diagnostics and environmental monitoring. Services ProvidedThe overall goal is to establish a center for distributing high-quality, well-characterized inbred, hybrid, and mutant rats to investigators. To this end, the RRRC will select and import rat strains and stocks important to the biomedical research community; rederive rats to a pathogen-free state; cryopreserve gametes and embryos; perform genotyping, phenotyping, and infectious disease monitoring to ensure the quality of the rats; and distribute rats to investigators as live rats, cryopreserved germplasm, or tissues. Contact Information
University of Missouri
Web site: www.nrrrc.missouri.edu Grant No.: P40 RR016939
Principal Investigator
Additional Contacts
Beth Bauer, D.V.M. Special Mouse Strains ResourceResearch Emphasis/ObjectivesThe Special Mouse Strains Resource (SMSR) is a resource of special strains of mice that are valuable tools for genetic analysis of complex diseases. They include panels of recombinant inbred and chromosome substitution strains with diverse genetic backgrounds. The mission of the SMSR is to provide these strains of mice to interested investigators and to enhance their value as scientific tools with additional genotyping and phenotyping. Current ResearchThe SMSR maintains, characterizes and distributes mouse strain panels of recombinant inbred (RI) strains and chromosome substitution (consomic) strains. Holdings as of December 2006 include five RI strain panels and two chromosome substitution panels, one with A/J chromosomes on the C57BL/6J genetic background (made by Joseph Nadeau, Case Western Reserve University) and one with PWD chromosomes on the C57BL/6J background (made by Jiri Forejt in Czechoslovakia). PWD is a pure Mus musculus musculus inbred strain. The Nadeau consomic panel has been characterized for immunological, hematological, behavioral, skeletal, auditory, ocular, and physiological phenotypes to add to baseline information. The Forejt panel is being characterized for many of these same phenotypes. All of these data will be made publicly available online in the Phenome database http://phenome.jax.org/pub-cgi/phenome/mpdcgi?rtn=docs/home as analyses are completed. Services Provided
The resource provides technical support for users of JAX mice to
answer questions regarding genetics, husbandry, and characteristics of
mutant mice. All mice can be ordered by calling The Jackson
Laboratory's Customer Service Department at 1-800-422-MICE or
207-288-5845 or by faxing 207-288-6150. Access the searchable database Contact Information
The Jackson Laboratory
Web site: www.jax.org Grant No.: P40 RR16049
Principal Investigator
Additional Contacts
Jeffrey P. Lake, Ph.D. |
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National Institutes of Health (NIH) Bethesda, Maryland 20892 |
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Department of Health and Human Services |
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