Receptors and Signal Transduction Section, OIIB
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| Model of FceRI signaling |
NATIONAL INSTITUTES OF HEALTH/NIDCR
BUILDING 10 ROOM 1N106
10 CONVENT DR MSC 1188
BETHESDA MD 20892-1188
Phone: (301) 496-5105
Fax: (301) 480-8328
E-mail: rsiragan@mail.nih.gov
Research Interests
My interest is to characterize the molecular mechanisms involved in signal transduction in mast cells. These cells play an important role in many inflammatory and immunological reactions by releasing an array of mediators that include preformed and newly generated molecules, and cytokines.
In our studies we utilize cellular and molecular techniques in investigations with the rat basophilic leukemia cells and bone marrow derived mast cells. We have demonstrated that the protein tyrosine kinase Syk is essential for the immune receptor induced signaling and studies have functionally dissected the role of Syk in these signaling pathways. Present studies are characterizing further these signaling pathways.
M.D. State University of New York, Downstate Medical Center 1962
Ph.D. Johns Hopkins University, 1968
Education
1962, M.D. State University of New York, Downstate Medical Center
1968, Ph.D. Johns Hopkins University
Recent publications
Kalesnikoff, J., Huber, M., Lam, V., Damen, J.E., Zhang, J., Siraganian, R.P. and Krystal, G.: Monomeric IgE stimulates signaling pathways in mast cells that lead to cytokine production and cell survival. Immunity 14:801-811, 2001.
Xie, Z-H., Ambudkar I. and Siraganian, R.P.: The adaptor molecule Gab2 regulates FceRI-mediated signal transduction in mast cells. J. Immunology 168:4682-4691, 2002.
Siraganian, R.P., Zhang, J., Suzuki, K. and Sada, K.: Protein tyrosine Syk in mast cell signaling. Molecular Immunology 38:1229-1233, 2002.
Zhang, J., Berenstein, E. and Siraganian, R.P.: Phosphorylation of Tyr342 in the linker region of Syk is critical for FceRI signaling in mast cells. Molecular and Cellular Biology 22:8144-8154, 2002.
Vial D., Oliver C., Jamur M.C., Pastor M.V.D., da Silva Trindade E., Berenstein E.H., Zhang J. and Siraganian, R.P.: Alterations in granule matrix and cell surface of focal adhesion kinase-deficient mast cells. Journal of Immunology 171:6178-6186, 2003.
Siraganian, R.P.: Mast cell signal transduction from the high-affinity IgE receptor. Current Opinion in Immunology 15:639-646, 2003.
Zhang, J., Chiang, Y.J., Hodes, R.J. and Siraganian, R.P.: Inactivation of c-Cbl and Cbl-b differentially affects signaling from the high affinity IgE receptor. Journal of Immunology 173:1811-1818, 2004.
Hitomi T., Zhang J., Nicoletti L.M., Grodzki A.C.G., Jamur M.C., Oliver C., and Siraganian R.P.: Phospholipase D1 regulates high affinity IgE receptor-induced mast cell degranulation. Blood 104:4122-4128, 2004.
Jamur, M.C., Grodzki, A.C.G., Berenstein, E.H., Hamawy, M.M., Siraganian, R.P. and Oliver, C.: Identification and characterization of undifferentiated mast cells in mouse bone marrow. Blood 105:4282-4289, 2005.
Zhang, J., Suzuki, K., Hitomi, T and Siraganian, R.P.: TOM1L1 is a Lyn substrate involved in FceRI signaling in mast cells. Journal of Biological Chemistry 282:37669-37677, 2007.