John A. Chiorini

Adeno-associated virus, vector development, Sjögren's Syndrome

Drapkin PT, O¹Riordan CR, Yi SM, Chiorini JA, Cardella J, Zabner J, Welsh MJ. Targeting the urokinase plasminogen activator receptor enhances gene transfer to human airway epithelia. J Clin Invest 105(5):589-596, 2000.

Zabner J, Seiler M, Walters RW, Kotin RM, Fulgeras W, Davidson BL, Chiorini JA. Adeno-associated virus type 5 (AAV5) but not AAV2 binds to the apical surface of airway epithelia and facilitates gene transfer. J Virol 74(8):3852-3858, 2000.

Alisky JM, Hughes SM, Sauter SL, Jolly D, Dubensky TW Jr, Staber PD, Chiorini JA, Davidson BL. Transduction of murine cerebellar neurons with recombinant FIV and AAV5 vectors.Neuroreport. 11(12): 2669-73. 2000.

Walters RM, Yi SM, Keshavjee S, Brown KE, Welsh MJ, Chiorini JA, Zabner J.  Binding of adeno-associated virus type 5 to 2,3-linked sialic acid is required for gene transfer. J Biol Chem 276(23):20610-20616, 2001.

Kaludov N, Brown KE, Walters RW, Zabner J, Chiorini JA. AAV4 and AAV5 both require sialic acid binding for hemagglutination and efficient transduction but differ in sialic acid linkage specificity. J Virol 75(15):6884-6893, 2001.

Yamano S, Scott DE, Huang LY, Mikolajczyk M, Pillemer SR, Chiorini JA, Golding B, Baum BJ. Protection from experimental endotoxemia by a recombinant adeno-associated virus encoding interluekin 10. J Gene Med 3(5):450-457, 2001.

Schmidt M, Chiorini JA, Afione S, Kotin R. Adeno-associated virus type 2 Rep78 inhibition of PKA and PrKX: fine mapping and analysis of mechanism. J Virol 76(3):1033-1042, 2002.

Yamano S, Huang LY, Ding C, Chiorini JA, Goldsmith CM, Wellner RB, Golding B, Kotin RM, Scott DE, Baum BJ. Recombinant adeno-associated virus serotype 2 vectors mediate stable interleukin 10 secretion from salivary glands into the bloodstream. Hum Gene Ther 13(2):287-298, 2002.

Walters RW, Pilweski JM, Chiorini JA, Zabner J. Secreted and transmembrane mucins inhibit gene transfer with AAV4 more efficiently than AAV5. J Biol Chem 277(26):23709-23713, 2002.

Kaludov N, Handelman B, Chiorini JA. Scalable purification of adeno-associated virus type 2, 4, or 5 using ion-exchange chromatography. Hum Gene Ther 13(10):1235-1243, 2002.

Rooney CP, Denning GM, Davis BP, Flaherty DM, Chiorini JA, Zabner J. Bronchoalveolar fluid is not a major hindrance to virus-mediated gene therapy in cystic fibrosis. J Virol 76(20):10437-10443, 2002.

Haskell RE, Hughes SM, Chiorini JA, Alisky JM, Davidson BL. Viral-mediated delivery of the late-infantile neuronal ceroid-lipofuscinosis gene, TPP-1 to the mouse central nervous system. Gene Ther 10(1):34-42, 2003.

Kaludov N, Padron E, Govindasamy L, McKenna R, Chiorini JA, Agbandje-McKenna M. Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 4.Virology. Feb 1;306(1):1-6, 2003.

Di Pasquale G, Chiorini JA. PKA/PrKX activity is a modulator of AAV/adenovirus interaction. EMBO J. 22:1716-24. 2003.  Smith-Arica JR, Thomson AJ, Ansell R, Chiorini J, Davidson B, McWhir J.  Infection efficiency of human and mouse embryonic stem cells using adenoviral and adeno-associated viral vectors. Cloning Stem Cells.  5(1):51-62, 2003.

Bossis I, Chiorini JA. Cloning of an avian adeno-associated virus (AAAV) and generation of recombinant AAAV particles. J Virol. 77:6799-810, 2003.

Pasquale GD, Davidson BL, Stein CS, Martins I, Scudiero D, Monks A, Chiorini JA. Identification of PDGFR as a receptor for AAV-5 transduction. Nat Med.  9:1306-12, 2003.

Kok MR, Yamano S, Lodde BM, Wang J, Couwenhoven RI, Yakar S, Voutetakis A, Leroith D, Schmidt M, Afione S, Pillemer SR, Tsutsui MT, Tak PP, Chiorini JA, Baum BJ. Local adeno-associated virus-mediated interleukin 10 gene transfer has disease-modifying effects in a murine model of Sjogren's syndrome. Hum Gene Ther. 14:1605-18, 2003.

Lotery AJ, Yang GS, Mullins RF, Russell SR, Schmidt M, Stone EM, Lindbloom JD, Chiorini JA, Kotin RM, Davidson BL. Adeno-associated virus type 5: transduction efficiency and cell-type specificity in the primate retina.Hum Gene Ther. 14:1663-71, 2003.

Voutetakis A, Kok MR, Zheng C, Bossis I, Wang J, Cotrim AP, Marracino N,Goldsmith CM, Chiorini JA, Loh YP, Nieman LK, Baum BJ. Reengineered salivary glands are stable endogenous bioreactors for systemicgene therapeutics.Proc Natl Acad Sci U S A Mar 2;101(9):3053-8, 2004.

Walters RW, Agbandje-McKenna M, Bowman VD, Moninger TO, Olson NH, Seiler M,Chiorini JA, Baker TS, Zabner J. Structure of adeno-associated virus serotype 5. J Virol. Apr;78(7):3361-71, 2004.

Katano H, Afione S, Schmidt M, Chiorini JA. Identification of adeno-associated virus contamination in cell and virus stocks by PCR.  Biotechniques. Apr;36(4):676-80, 2004.

Schmidt M, Katano H, Bossis I, Chiorini JA. Cloning and characterization of a bovine adeno-associated virus. J Virol. Jun;78(12):6509-16, 2004.

Kok MR, Voutetakis A, Yamano S, Wang J, Cotrim A, Katano H, Bossis I, Chiorini JA, Tran SD, Tak PP, Baum BJ. Immune responses following salivary gland administration of recombinant adeno-associated virus serotype 2 vectors. J Gene Med.Apr;7(4):432-41, 2005.

Ostedgaard LS, Rokhlina T, Karp PH, Lashmit P, Afione S, Schmidt M, Zabner J, Stinski MF, Chiorini JA, Welsh MJ. A shortened adeno-associated virus expression cassette for CFTR gene transfer to cystic fibrosis airway epithelia. Proc Natl Acad Sci U S A. Feb 22;102(8):2952-7, 2005.

Padron E, Bowman V, Kaludov N, Govindasamy L, Levy H, Nick P, McKenna R, Muzyczka N, Chiorini JA, Baker TS, Agbandje-McKenna M.Structure of adeno-associated virus type 4. J Virol. Apr;79(8):5047-58, 2005.

Di Pasquale G, Rzadzinska A, Schneider ME, Bossis I, Chiorini JA, Kachar B.  A novel bovine virus efficiently transduces inner ear neuroepithelial cells.  Mol Ther. Jun;11(6):849-55, 2005.

Voutetakis A, Bossis I, Kok MR, Zhang W, Wang J, Cotrim AP, Zheng C, Chiorini JA, Nieman LK, Baum BJ.Salivary glands as a potential gene transfer target for gene therapeutics of some monogenetic endocrine disorders. J Endocrinol. Jun;185(3):363-72, 2005.

Liu G, Martins IH, Chiorini JA, Davidson BL.Adeno-associated virus type 4  (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS.  Gene Ther. Oct;12(20):1503-8, 2005.

Liu G, Martins I, Wemmie JA, Chiorini JA, Davidson BL.Functional correction of CNS phenotypes in a lysosomal storage disease model using adeno-associated virus type 4 vectors. J Neurosci. Oct 12;25(41):9321-7, 2005.

DiMattia M, Govindasamy L, Levy HC, Gurda-Whitaker B, Kalina A, Kohlbrenner E, Chiorini JA, McKenna R, Muzyczka N, Zolotukhin S, Agbandje-McKenna M.  Production, purification, crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 5. Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1;61(Pt 10):917-21, 2005.

Katano H, Kok MR, Cotrim AP, Yamano S, Schmidt M, Afione S, Baum BJ, Chiorini JA.Enhanced transduction of mouse salivary glands with AAV5-based vectors. Gene Ther. Apr;13(7):594-601, 2006.

Di Pasquale G, Chiorini JA.AAV transcytosis through barrier epithelia and endothelium. Mol Ther. Mar;13(3):506-16, 2006.

Li JZ, Li H, Hankins GR, Lieu AS, Noh E, Jacobson L, Pittman DD, Chiorini JA, Helm GA. Different osteogenic potentials of recombinant human BMP-6 adeno-associated virus and adenovirus in two rat strains. Tissue Eng.  Feb;12(2):209-19, 2006.

Schmidt M, Grot E, Cervenka P, Wainer S, Buck C, Chiorini JA. Identification and characterization of novel adeno-associated virus isolates in ATCC virus stocks. J Virol. May;80(10): 5082-5, 2006.

Schmidt M, Chiorini JA.Gangliosides are essential for bovine adeno-associated virus entry. J Virol. Jun;80(11):5516-22, 2006.

Govindasamy L, Padron E, McKenna R, Muzyczka N, Kaludov N, Chiorini JA, Agbandje-McKenna M.Structurally mapping the diverse phenotype of adeno-associated virus serotype 4. J Virol. Dec;80(23): 11556-70, 2006.

Zheng C, Goldsmith CM, Mineshiba F, Chiorini JA, Kerr A, Wenk ML, Vallant M, Irwin RD, Baum BJ.Toxicity and biodistribution of a first-generation recombinant adenoviral vector, encoding aquaporin-1, after retroductal delivery to a single rat submandibular gland. Hum Gene Ther.  Nov;17(11):1122-33, 2006

Liu G, Chen YH, He X, Martins I, Heth JA, Chiorini JA, Davidson BL.  Adeno-associated virus type 5 reduces learning deficits and restores glutamate receptor subunit levels in MPS VII mice CNS. Mol Ther.  Feb;15(2):242-7, 2007.

Voutetakis A, Zheng C, Mineshiba F, Cotrim AP, Goldsmith CM, Schmidt M, Afione S, Roescher N, Metzger M, Eckhaus MA, Chiorini JA, Dunbar CE, Donahue RE, Baum BJ. Adeno-associated virus serotype 2-mediated gene transfer to the parotid glands of nonhuman primates. Hum Gene Ther. Feb;18(2):142-50, 2007.

Tran SD, Sugito T, Dipasquale G, Cotrim AP, Bandyopadhyay BC, Riddle K, Mooney D, Kok MR, Chiorini JA, Baum BJ.Re-engineering primary epithelial cells from rhesus monkey parotid glands for use in developing an artificial salivary gland. Tissue Eng. Oct;12(10):2939-48, 2006.

Voutetakis A, Zheng C, Wang J, Goldsmith CM, Afione S, Chiorini JA, Wenk ML, Vallant M, Irwin RD, Baum BJ. Gender differences in serotype 2 adeno-associated virus biodistribution after administration to rodent salivary glands. Hum Gene Ther. Nov;18(11):1109-18, 2007.

Schmidt M, Voutetakis A, Afione S, Zheng C, Mandikian D, Chiorini JA.  Adeno-associated virus type 12 (AAV12): a novel AAV serotype with sialic acid- and heparan sulfate proteoglycan-independent transduction activity. J Virol. Feb;82(3):1399-406, 2008.

Zheng C, Vitolo JM, Zhang W, Mineshiba F, Chiorini JA, Baum BJ. Extended transgene expression from a nonintegrating adenoviral vector containing retroviral elements.Mol Ther. Jun;16(6):1089-97, 2008.

Schmidt M, Govindasamy L, Afione S, Kaludov N, Agbandje-McKenna M, Chiorini JA. Molecular characterization of the heparin dependent transduction domain on the capsid of a novel Adeno-associated virus isolate, AAV(VR-942). J Virol. Jun 4. In Press, 2008.