Yoshihiko Yamada, PhD

Molecular Biology Section, LCDB

NATIONAL INSTITUTES OF HEALTH/NIDCRBUILDING 30 ROOM 40730 CONVENT DR MSC 4370BETHESDA MD 20892-4370

Phone: (301) 496-2111Fax: (301) 402-0897E-mail: Dr. Yoshihiko Yamada

Research Interests

Molecular mechanisms of gene expression in development and diseases; roles of extracellular matrix for organogenesis and tissue regeneration; novel genes important for craniofacial and tooth development; basement membrane and cartilage; transcription factors, laminin, collagen, and proteoglycan.

Education

1966: BS, Biology, Osaka University, Faculty of Science1968: MS, Biology, Osaka University, Faculty of Science1971: PhD, Biology, Osaka University, Faculty of Science

Recent publications

Arikawa-Hirasawa, E., Watanabe, H., Takami, H., Hassell, J.R., Yamada, Y. Perlecan is essential for cartilage and cephalic development. Nat. Genet. 23(3): 354-358, 1999.Kuratomi, Y., Nomizu, M., Nielsen, P.K., Tanaka, K., Song, S.Y., Kleinman, H.K., Yamada, Y. Identification of metastasis-promoting sequences in the mouse laminin alpha-1 chain. Exp. Cell Res. 249(2): 386-395, 1999.Watanabe, H., Yamada, Y. Mice lacking link protein develop dwarfism and craniofacial abnormalities. Nat. Genet. 21(2): 225-229, 1999.Fleischmajer, R., Kuroda, K., Hazan, R., Gordon, R.E., Lebwohl, M.G., Sapadin, A.N., Unda, F., Iehara, N., Yamada, Y. Basement membrane alterations in psoriasis are accompanied by epidermal overexpression of MMP-2 and its inhibitor TIMP-2. J. Invest. Dermatol. 115(5): 771-777, 2000.Fleischmajer, R., Kuroda, K., Utani, A., Douglas 2nd, M.E., Perlish, J.S., Arikawa-Hirasawa, E., Sekiguchi, K., Sanzen, N., Timpl, R., Yamada, Y. Differential expression of laminin alpha chains during proliferative and differentiation stages in a model for skin morphogenesis. Matrix Biol. 19(7): 637-647, 2000.Nielsen, P.K., Gho, Y.S., Hoffman, M.P., Watanabe, H., Makino, M., Nomizu, M., Yamada, Y. Identification of a major heparin and cell binding site in the LG4 module of the laminin alpha-5 chain. J. Biol. Chem. 275(19): 14517-14523, 2000.Arikawa-Hirasawa, E., Wilcox, W.R., Le, A.H., Silverman, N., Govindraj, P., Hassell, J.R., Yamada, Y. Dyssegmental dysplasia, Silverman-Handmaker type, is caused by functional null mutations of the perlecan gene. Nat. Genet. 27(4): 431-434, 2001.Arikawa-Hirasawa, E., Wilcox, W.R., Yamada, Y. Dyssegmental dysplasia, Silverman-Handmaker type: Unexpected role of perlecan in cartilage development. Amer. J. Med. Genetics. 106: 254-257, 2001.Nielsen, P.K., Yamada, Y. Identification of cell-binding sites on the Laminin alpha-5 N-terminal domain by site-directed mutagenesis. J. Biol. Chem. 276(14): 10906-10912, 2001.Watanabe, H., de Caestecker, M.P., Yamada, Y. Transcriptional cross-talk between Smad, ERK1/2, and p38 mitogen-activated protein kinase pathways regulates transforming growth factor-beta -induced aggrecan gene expression in chondrogenic ATDC5 cells. J. Biol. Chem. 276(17): 14466-14473, 2001.Arikawa-Hirasawa, E., Le, A.H., Nishino, I., Nonaka, I., Ho, N.C., Francomano, C.A., Govindraj, P., Hassell, J.R., Devaney, J.M., Spranger, J., Stevenson, R.E., Iannaccone, S., Dalakas, M.C., Yamada, Y. Structural and functional mutations of the perlecan gene cause schwartz-jampel syndrome, with myotonic myopathy and chondrodysplasia. Am. J. Hum. Genet. 70(5): 1368-1375, 2002.Arikawa-Hirasawa, E., Rossi, S.G., Rotundo, R.L., Yamada, Y. Absence of acetylcholinesterase at the neuromuscular junctions of perlecan-null mice. Nat. Neurosci. 5(2): 119-123, 2002.Fukumoto, S., Kiba, T., Hall, B., Iehara, N., Nakamura, T., Longenecker, G., Krebsbach, P.H., Nanci, A., Kulkarni, A.B., Yamada, Y. Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts. J. Cell Biol. 167(5): 973-983, 2004.Nakamura, T., Unda, F., de-Vega, S., Vilaxa, A., Fukumoto, S., Yamada, K.M., Yamada, Y. The Krüppel-like factor epiprofin is expressed by epithelium of developing teeth, hair follicles, and limb buds and promotes cell proliferation. J. Biol. Chem. 279(1): 626-634, 2004.Fukumoto, S., Miner, J.H., Ida, H., Fukumoto, E., Yuasa, K., Miyazaki, H., Hoffman, M.P., Yamada, Y. Laminin alpha5 is required for dental epithelium growth and polarity and the development of tooth bud and shape. J. Biol. Chem. 281(8): 5008-5016, 2006.Hozumi, K., Suzuki, N., Nielsen, P.K., Nomizu, M., Yamada, Y. Laminin alpha1 chain LG4 module promotes cell attachment through syndecans and cell spreading through integrin alpha2beta1. J. Biol. Chem. 281(43): 32929-32940, 2006.de Vega, S., Iwamoto, T., Nakamura, T., Hozumi, K., McKnight, D.A., Fisher, L.W., Fukumoto, S., Yamada, Y. TM14 is a new member of the fibulin family (fibulin-7) that interacts with extracellular matrix molecules and is active for cell binding. J. Biol. Chem. 2007. In press.