Molecular Probes for Identification or Isolation of Membrane Proteins
Background:
The Nanobiology Program of the
National Cancer Institute is seeking statements of capability or
interest from parties interested in collaborative research to
obtain pre-clinical data to be used to further develop, evaluate,
or commercialize molecular probes designed around an iodonaphthyl
succinate antigen.
Technology:
This technology describes a new
class of molecular probes designed around an iodonaphthyl succinate
antigen. The probes can be used to label and tag proteins using a
variety of conventional protein modification chemistries. The
technology is offered as a combination of probe + monoclonal
antibodies against the probe (three clones). Several possible
probes as well as monoclonal antibodies that react with these
probes are described in this invention.
The probe can be used for labeling and tagging cell surface and
integral membrane proteins as well as soluble proteins. The
monoclonal antibodies were tested and found to be effective for
immunoprecipitation, western blot, and flow cytometry. Once tagged,
the modified proteins can be detected or isolated using an antibody
reactive with the probe. These probes and their corresponding
antibodies have significant advantages over the biotin-avidin
system, including the ability to reverse binding for protein
isolation and the lack of high, non-specific binding to cell
surfaces. Additionally, an isotopic125l label may be
incorporated in the probe for tracking tagged proteins in
vivo
Further R&D
Needed:
Organic Synthesis of derivatives that are selective towards the
lipophilic domain biological membranes.
R&D Status:
In vitro data is available
IP Status:
U.S. Provisional Application No. 60/906,166 filed 09 Mar 2007
Value Proposition:
- reversibility of binding for protein isolation
- lack of high, non-specific binding to cell surfaces
- ability to incorporate isotopicisotopic125l label in
the probe for tracking tagged proteins in vivo
Contact
Information:
John D. Hewes, Ph.D., NCI
Technology Transfer Center
Phone: 301-435-3121
E-mail: Hewesj@mail.nih.gov
Reference: #511 JH
Posted 12/17/2007