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Sponsors and Collaborators: |
Albert Einstein College of Medicine of Yeshiva University Juvenile Diabetes Research Foundation National Institutes of Health (NIH) |
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Information provided by: | Albert Einstein College of Medicine of Yeshiva University |
ClinicalTrials.gov Identifier: | NCT00703989 |
Benfotiamine blocks three major pathways of hyperglycemic damage and prevents experimental diabetic retinopathy and incipient nephropathy in these models. In cultured vascular cells, it also reduces aldose reductase gene expression, activity, and sorbitol levels. It does so by activating the enzyme transketolase. α-lipoic acid, a potent antioxidant, has also been reported to reduce both diabetic microvascular and macrovascular complications in animal models. To determine whether benfotiamine in combination with α-lipoic acid would normalize markers of ROS-induced pathways of complications in humans, we performed a pilot study in subjects with Type 1 diabetes using one daily dose of benfotiamine in combination with α-lipoic acid.
Condition | Intervention |
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Type 1 Diabetes |
Dietary Supplement: benfotiamine, α-lipoic acid |
Study Type: | Interventional |
Study Design: | Treatment, Non-Randomized, Open Label, Active Control, Single Group Assignment, Efficacy Study |
Official Title: | The Role of the Glucosamine Pathway and Reactive Oxygen Species in the Pathogenesis of Diabetic Complications |
Enrollment: | 21 |
Study Start Date: | February 2005 |
Study Completion Date: | February 2008 |
Primary Completion Date: | October 2006 (Final data collection date for primary outcome measure) |
Arms | Assigned Interventions |
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I: Experimental
Patients with Type 1 diabetes
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Dietary Supplement: benfotiamine, α-lipoic acid
benfotiamine 300 mg twice a day, (Advanced Orthomolecular Research, Calgary, AB,CANADA) and slow-release α-lipoic acid (600 mg twice a day) (MRI, San Francisco, CA) for a total duration of four weeks
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II: No Intervention
Age-matched male subjects without Type 1 diabetes
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The glycemic status of study patients was assessed by measuring baseline values of HbA1c, fructosamine, and fasting plasma glucose. Mean HbA1c was 8.7+ 0.7%, mean fructosamine was 421+29 mg/dl (normal range 174-286 mg/dl), and mean fasting blood glucose was 198+44 mg/dl.
At day 0, subjects levels of markers of two benfotiamine-sensitive pathways were determined: intracellular advanced glycation endproduct (AGE) formation, as reflected by a marker of increased intracellular methylglyoxal adducts in endothelial cells, angiopoietin 2 and hexosamine pathway activity, measured by determination of N-acetylglucosamine-modified protein in circulating monocytes. PKC activity in circulating monocytes could not be measured because the amount of blood required exceeded that approved by the Committee on Clinical Investigations. Serum levels of 6-keto-PGF-1 , a stable product produced by the nonenzymatic hydration of the antiatherogenic mediator prostacyclin were also determined. Subjects then took benfotiamine 300 mg twice a day, (Advanced Orthomolecular Research, Calgary, AB,CANADA) and slow-release α-lipoic acid (600 mg twice a day) (MRI, San Francisco, CA) for 28 days. Blood was obtained at day 0, day 15, and day 28.
Data were analyzed using 1-factor analysis of variance to compare the means of all the groups. The Tukey−Kramer multiple comparisons procedure was used to determine which pairs of means were different.
Ages Eligible for Study: | 18 Years to 45 Years |
Genders Eligible for Study: | Male |
Accepts Healthy Volunteers: | Yes |
Inclusion Criteria:
Exclusion Criteria:
United States, New York | |
GCRC, Albert Einstein College of Medicine | |
Bronx, New York, United States, 10461 |
Principal Investigator: | Michael Brownlee, M.D. | Albert Einstein College of Medicine of Yeshiva University |
Responsible Party: | Albert Einstein College of Medicien ( Michael Brownlee, M.D. ) |
Study ID Numbers: | CCI#: 2004-582, JDRF grant #8-2003-784, GCRC grant # MO1-RR12248 |
Study First Received: | June 23, 2008 |
Last Updated: | June 23, 2008 |
ClinicalTrials.gov Identifier: | NCT00703989 |
Health Authority: | United States: Institutional Review Board |
hyperglycemia diabetic complications advanced glycation endproducts |
hexosamine pathway prostacyclin synthase reactive oxygen species |
Metabolic Diseases Autoimmune Diseases Diabetes Mellitus Endocrine System Diseases Hyperglycemia Diabetes Mellitus, Type 1 Benphothiamine |
Epoprostenol Endocrinopathy Glucose Metabolism Disorders Metabolic disorder Diabetes Complications Thioctic Acid |
Vitamin B Complex Antioxidants Immune System Diseases Molecular Mechanisms of Pharmacological Action Immunologic Factors Growth Substances Physiological Effects of Drugs |
Adjuvants, Immunologic Protective Agents Pharmacologic Actions Vitamins Micronutrients Chelating Agents |