• Predictive significance of melanoma susceptibility gene (MSG) mutations in the CDKN2A gene [ Designated as safety issue: No ]
  
• Susceptibility to other types of cancer as a feature of MSG mutations [ Designated as safety issue: No ]
  
• Risk of other types of cancers in mutation carriers [ Designated as safety issue: No ]
  
• Environmental exposures, in particular sun exposure, that modify risk of melanoma in MSG mutation carriers [ Designated as safety issue: No ]
  

OBJECTIVES:

• Determine the incidence and etiologic significance of variants of known melanoma susceptibility genes (MSGs) in families with multiple cases of melanoma.
• Determine the proportion of multiple-case families that are explained by high-penetrance mutations in known MSGs.
• Determine the proportion of multiple-case families that are explained by these mutations and whether it varies with latitude, as a surrogate for ultraviolet exposure, with number of affected relatives, with average age at onset of melanoma in relatives, with presence of multiple primary melanoma, or with other family-specific variables.
• Determine the penetrance of MSG mutations in these families.
• Determine if the penetrance varies with age, sex, or birth cohort.
• Determine if the penetrance varies with the gene involved or nature of the mutation.
• Assess the penetrance in mutations that also have a deleterious effect on the alternative splice product, p14ARF.
• Determine whether carriers of MSGs have an increased susceptibility to other types of cancer.
• Determine the risk of other types of cancers for mutation carriers.
• Determine environmental exposures, in particular sun exposure, that modify risk of melanoma in MSG mutation carriers.
• Determine the cutaneous phenotypes that correlate with melanoma risk in these families.
• Correlate cutaneous phenotypes with the presence of MSG variants.
• Determine the effect of other covariates, such as sun exposure or the presence of alleles of putative modifying genes (e.g., MC1R or CDKN2A), on phenotype.
• Determine if modifier genes, such as those controlling pigmentation of the skin, and therefore sun susceptibility, modify risk in MSG mutation carriers.
• Identify any histopathological correlates of MSG status in primary tumors arising in melanoma-susceptible individuals in these families.
• Identify any histopathological correlates of primary melanomas in carriers of MSG mutations with other covariates.

OUTLINE: This is a case-control, multicenter study.

Participants complete 2 questionnaires and assist in the creation and expansion of a family pedigree. Blood samples are examined for melanoma susceptibility gene mutations, including CDK4 and CDKN2A.

Participants are also examined for moles and photographed. Physical variables (e.g., skin, eye, and hair pigmentation) and sun damage (solar lentigines and freckling) are also noted.

If available, tissue samples are examined for Clark level, Breslow thickness, and frequency of mitoses. Peri-lesional skin from tumors is examined by p53 staining.

Participants are followed periodically to monitor cancer development.

Peer reviewed and funded or endorsed by Cancer Research UK

PROJECTED ACCRUAL: A total of 5,000 participants will be accrued for this study.