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Sponsored by: |
The Catholic University of Korea |
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Information provided by: | The Catholic University of Korea |
ClinicalTrials.gov Identifier: | NCT00570362 |
The purpose of this study is to evaluate whether systemic glutathione level is decreased in patients with normal tension glaucoma.
Condition |
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Normal Tension Glaucoma |
Study Type: | Observational |
Study Design: | Case Control, Cross-Sectional |
Official Title: | Evaluation of Systemic Glutathione Level in Patients With Normal Tension Glaucoma |
serum
Estimated Enrollment: | 60 |
Study Start Date: | March 2008 |
Estimated Study Completion Date: | October 2008 |
Normal tension glaucoma is one of the most common cause of primary open angle glaucoma in Korea. Even though, we still do not know what the cause is. Only IOP-lowering drugs are the currently available therapeutic method. Glutathione is one of the mostly high concentrated intracellular antioxidants. We find apoptosis of neuronal cell in the mouse retina by systemic depletion of glutathione using buthionine sulfoximine. Glutathione is also known to be reduced in primary open angle glaucoma with high IOP. So we are planning to evaluate systemic glutathione level in normal tension glaucoma.
Blood samples will be obtained by venipuncture to the antecubital vein of normal tension glaucoma patients and age-matched normal controls. Five milliliters of blood will be collected in EDTA-treated tubes (to prevent oxidation). Thirty microliters of blood will be then transferred into centrifuge tubes, and will be added 33.3 microliter of 5-sulfosalicylic acid (SSA), 100mg/mL within 10 minutes from the blood collection.Each sample will be then diluted with 936.7 microliter sodium phosphate buffer (pH 7.5), and the content of each tube will be mixed rapidly in a centrifuge at 13,000 rpm for 5 minutes. A portion of supernatant (150 microliter) will be then collected into clean centrifuge tubes and immediately cooled at -70°C. To each well of a 96-well plate, 150 microliter of daily buffer (3 mg NADPH into 10 mL sodium phosphate buffer), 50microliter of DTNB solution, and 25 microliter of standards or samples will be added in quadruplicate, and the plate will be incubated at 37 °C for 3 minutes.Finally, 25 microliter GSSGR will be added to the previous mixture, and the plate will be read at 410 nm using a 96-well plate reader(UV spectrophotometer).
Ages Eligible for Study: | 18 Years to 80 Years |
Genders Eligible for Study: | Both |
Accepts Healthy Volunteers: | Yes |
Sampling Method: | Non-Probability Sample |
patients of tertiary referred center
Inclusion Criteria:
Exclusion Criteria:
The control group:
Exclusion criteria for both groups:
Korea, Republic of | |
College of Medicine, The Catholic University of Korea, St. Mary's Hospital | |
Seoul, Korea, Republic of, 150-713 |
Study Director: | Jung-Il Moon, Professor | Department of Ophthalmology, St.Mary's Hospital, College of Medicine, The Catholic University of Korea |
Responsible Party: | St.Mary's Hospital, College of Medicine, The Catholic University of Korea ( Jung-Il Moon ) |
Study ID Numbers: | Merck-001, CMCEYE-GSH |
Study First Received: | December 7, 2007 |
Last Updated: | December 7, 2007 |
ClinicalTrials.gov Identifier: | NCT00570362 |
Health Authority: | Korea: Food and Drug Administration |
Normal tension glaucoma Glutathione Antioxidant |
Glaucoma Eye Diseases Hypertension Ocular Hypertension |