Nanoscale Detection Macromolecules

Background:
The National Cancer Institute, Center for Cancer Research Nanobiology Program seeks a research partnership to develop devices that can be used to detect and/or selectively modify biomolecules. Possible uses of the nanoprobes include detection of a wide variety of clinical and biowarfare reagents; or to rapidly and simply detect, modify and/or destroy endogenous molecules such as proteins and mRNA involved in a broad range of diseases.

Technology:
The nanoprobes consist of a rigid molecular rod with flexible molecular tethers attached at each end. Each tether has a functional group at the tip. For example, one tip could be a donor fluorophore and the other could be an acceptor fluorophore. The fluorophores form a pair for Forster Resonance Energy Transfer (FRET). In the absence of the target molecule, the rod keeps the tethers apart most of the time, while in the presence of the target molecule, both tips bind to the target. This holds the donor and acceptor fluorophores close together. Subsequent illumination with light excites the donor and the energy is transferred by FRET to the nearby acceptor, which emits a detectable signal.

Possible Applications of Technology:

• Reduction of ELISA, Southern, Northern and Western assays to single molecules
• Single molecule detection of biomolecules

Current State of Development:
Proof of concept

Further R&D Required:
Prototype development

IP Status:

• Filed PCT application filed December, 2006
• US patent application filed December, 2006

Contact Information:
John D. Hewes, Ph.D., NCI Technology Transfer Center
Phone: 301-435-3121
E-mail: Hewesj@mail.nih.gov

Reference:  #245 JH

Updated 10/29/2007