What Makes it Tick? Attempts to Understand the Dynamics of the Ribosome using Cryo-Electron Microscopy |
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| Launch in standalone player | |
| Air date: | Wednesday, May 14, 2003, 3:00:00 PM |
| Category: | Wednesday Afternoon Lectures |
| Description: | The NIH Director's Wednesday Afternoon Lecture Series
Cryo-EM of single particles has become indispensable as a tool for visualizing the dynamics of macromolecular machines. Application of this technique to the ribosome has been particularly fruitful. Several snapshots of the elongation cycle, in the form of three-dimensional cryo-EM density maps, are now available at resolutions in the range of 8 to 15 A. These show the ribosome in different conformations. The largest of those changes involves a "ratchet" rotation of the small subunit relative to the large subunit. By "molding" the static x-ray structure into these density maps, we are now gathering information on the local dynamic rearrangements of RNAs and proteins underlying the global changes. |
| Author: | Joachim Frank, Ph.D., New York University |
| Runtime: | 01:05:08 |
| Rights: | This is a work of the United States Government. No copyright exists on this material. It may be disseminated freely. |
| Download: | Download
Video How to download a Videocast |
| CIT File ID: | 10241 |
| CIT Live ID: | 1895 |
| Permanent link: | http://videocast.nih.gov/launch.asp?10241 |