Mouse
Index >> Targeted Mutagenesis Resources
Bay Genomics
The major goal of BayGenomics is to identify genes relevant to cardiovascular
and pulmonary disease. We are using gene-trap vectors to inactivate thousands
of genes in mouse embryonic stem (ES) cells for the purpose of generating
knockout mice. We
make all of our ES cell lines readily available to the scientific research
community.
Fred
Hutchison Cancer Research Center
Gene Trap Mutagenesis We are using gene traps in embryonic stem
(ES) cells to identify genes regulated by growth factors. We have begun
large scale sequencing of ES cell clones and sequence tags are deposited
to NCBI's
dbGSS . Sequence searches for genes that we have trapped can
be performed by running a BLAST search
of the dbGSS database. A list of the genes we have trapped can
be found in our gene
trap database.
German
Gene Trap Consortium
The German Genetrap Consortium (GGTC) generates a reference library of
gene trap sequence tags (GTST) from insertional mutations generated in
mouse embryonic stem (ES) cells.The gene trap database represents a repository
of sequences produced in large scale ion or retroviral infections.gene
trap screen in ES cells using various gene trapping vectors which are
delivered either by electroporation or retroviral infections.
Manitoba
Institute of Cell Biology, Centre for Mammalian Functional Genomics
We have developed a process called Tagged-Sequence Mutagenesis to disrupt
genes expressed in mouse embryo-derived stem (ES) cells and to characterize
each mutation by direct DNA sequencing. Comparison of these sequence
tags (PSTs) with the existing databases identifies disruptions of known
genes or genes which may be related by homology or functional domains.
The ability to induce, characterize and maintain mutations in ES cells
circumvents many limitations associated with conventional mammalian genetics,
and will greatly increase the number of mutant alleles (typically loss
of function mutations) by which gene functions can be studied in mice
and in cell lines derived from such mice.
Gene
trap Consortium
A public resource of insertional mutations in mouse embryonic stem cells
Centre
For Modelling Human Disease Gene Trap Core, University of Toronto/Mount
Sinai Hospital
The Gene Trap Laboratory is a core facility of The Centre for Modeling
Human Disease. A database of in vitro expression patterns can now be
searched.
Induced
Mutant Resource
Genetically engineered and induced mutation mouse models for research
Lexicon
Genetics Corp. Lexicon Genetics is a biopharmaceutical company
focused on the discovery of breakthrough treatments for human disease.
We use proprietary gene knockout technology to systematically discover
the physiological functions of genes in mice and to identify which
corresponding human genes encode potential targets for therapeutic
intervention, or drug targets.
Deltagen Deltagen's
unique systems biology approach is designed to help clear the first and
major hurdle of drug target discovery: lead validation. Our high-throughput in
vivo mammalian knockout technology , systematic array analysis
and cell-based array analysis capabilities enable us to identify and
validate the utility of genomic targets within a living model at an extraordinary
scale and speed
Velocigene/Regeneron
Regeneron's Velocigene™ allows custom and precise manipulation
of very large sequences of DNA to produce a highly customized knock-out
of a specified target gene and accelerates the production of knock-out
and transgenic expression models without using either positive/negative
selection or isogenic DNA. In producing knock-out models, a color or
fluorescent marker is substituted in place of the actual gene sequence,
allowing for high-resolution visualization of precisely where the gene
is active in the body, during normal body functioning, as well as in
disease processes. Velocigene uses automated systems for most steps in
the process. Thus, Velocigene allows scientists to identify rapidly the
physical and biological effects of deleting or over-expressing the target
gene
Mouse
Knockout & Mutation Database Fully searchable database of phenotypic
information related to knockouts and classical mutations in mice ,
includingextensive links to MEDLINE on BioMedNet
Deltabase DeltaBase
is a comprehensive, fully featured searchable database of in vivo -derived
mammalian gene function information and gene targets. Built upon our
high-throughput mouse gene knockout technology and standardized phenotypic
analysis protocols, this cutting-edge tool can be used in the pharmaceutical
and biotechnology industries to identify drug targets, validate the therapeutic
potential of specific genes and accelerate the drug discovery process.
DeltaBase currently is licensed by nonexclusive agreement to such leading
global pharmaceutical companies as Pfizer, GlaxoSmithKline and Merck. |