Home
Search
Study Topics
Glossary
|
|
|
|
|
|
Sponsored by: |
University Hospital, Bordeaux |
---|---|
Information provided by: | University Hospital, Bordeaux |
ClinicalTrials.gov Identifier: | NCT00808730 |
Asthma is a frequent disease characterized by bronchial hyperresponsiveness, inflammation and remodelling. Bronchial remodelling is an abnormal repair process that contributes to the development of poorly reversible airway narrowing. It can appear very early in the evolution of the disease and involves an increased mass of bronchial smooth muscle (BSM). The mechanism of such an increase has been related with an increase in smooth muscle cell proliferation. Recently, we have demonstrated that, BSM increased proliferation is induced by an enhanced mitochondrial biogenesis in severe asthma (T. Trian et al. J Exp Med 2007). The objective of this study is to investigate the role of smooth muscle cell mitochondria in non severe asthma
Condition | Intervention |
---|---|
Asthma |
Procedure: fiberoptic fibroscopy |
Study Type: | Interventional |
Study Design: | Health Services Research, Open Label, Uncontrolled, Single Group Assignment |
Official Title: | Role of Mitochondria in Human Bronchial Smooth Muscle Remodeling in Non Severe Asthma |
Estimated Enrollment: | 30 |
Study Start Date: | January 2009 |
Estimated Study Completion Date: | June 2010 |
Estimated Primary Completion Date: | March 2009 (Final data collection date for primary outcome measure) |
Arms | Assigned Interventions |
---|---|
1: Experimental
fiberoptic fibroscopy
|
Procedure: fiberoptic fibroscopy
Bronchial specimens will be obtained by fiberoptic bronchoscopy within 15 days after the enrolment
|
Bronchial remodelling mainly involves an increased mass of bronchial smooth muscle (BSM), which is related with an increase proliferation of BSM cells. Recently, using BSM cells obtained from severe asthmatics, we have demonstrated that such an increase proliferation was induced by an activation cascade involving an abnormal calcium entry, and the subsequent activation of Calmodulin-kinase IV, PGC-1alpha, NRF-1 and mt-TFA leading to an increase mitochondrial biogenesis (T. Trian et al, J Exp Med 2007). The objective of this study is to investigate the role of BSM cell mitochondria in non severe asthma.
For this purpose, 30 non severe asthmatic adult patients (>18 yr) will be prospectively recruited from the "CHU de Bordeaux" according to the Global Initiative for Asthma (GINA) guidelines. Inclusion visit will include written informed consent, asthma control questionnaire, clinical examination, lung function testing (i.e. arterial gas, exhaled NO, plethysmography), prick tests, chest X Ray and blood sample for total IgE levels. Bronchial specimens will be obtained from all subjects by fiberoptic bronchoscopy. BSM remodelling will be evaluated by morphological analysis. Patients will be divided into 2 groups according to the presence or the absence of BSM remodelling. Using BSM cell culture, the role of mitochondria will be analyzed by electronic microscopy, confocal microscopy, immunoblotting, RT-PCR and oxygraphy.
Ages Eligible for Study: | 18 Years and older |
Genders Eligible for Study: | Both |
Accepts Healthy Volunteers: | No |
Inclusion Criteria:
Exclusion Criteria:
Contact: Pierre-Olivier Girodet, MCU-PH | 05 57 57 15 60 | pierre-olivier.girodet@chu-bordeaux.fr |
Contact: Patrick Berger, MCU-PH | 05 57 65 65 13 | patrick.berger@u-bordeaux2.fr |
France | |
University Hospital Bordeaux, Hôpital Haut-Lévêque | |
Pessac, France, 33604 |
Principal Investigator: | Pierre-Olivier GIRODET, MCU-PH | University Hospital Bordeaux / Département de Pharmacologie, CIC - Université Victor Segalen Bordeaux 2 |
Responsible Party: | University Hospital, Bordeaux ( Jean-Pierre LEROY / Clinical Research and Innovation Director ) |
Study ID Numbers: | CHUBX 2008/29 |
Study First Received: | December 15, 2008 |
Last Updated: | December 15, 2008 |
ClinicalTrials.gov Identifier: | NCT00808730 |
Health Authority: | France: Afssaps - French Health Products Safety Agency |
Asthma, airway remodelling, smooth muscle, mitochondria |
Hypersensitivity Lung Diseases, Obstructive Respiratory Tract Diseases Lung Diseases |
Hypersensitivity, Immediate Asthma Respiratory Hypersensitivity |
Immune System Diseases Bronchial Diseases |