Mice generated in LGP
Over the past two decades LGP has generated transgenic mice and targeted mice, which have been used by researchers around the globe. The mice shown below are available from various sources, including the Jackson lab and the NCI repository. Because of limited resources we are currently not be able to ship mice. Since the mice have been obtained many labs in the US, Europe and Asia these could serve as distribution centers. Labs who would like to obtain mice from LGP should send their requests to Lothar Hennighausen and sign a standard MTA (more info at the end of this page).
Cre expressing mice
The MMTV-LTR targets gene expression primarily to mammary epithelium but also to other cell types. Two lines are available, the A and the D line Wagner et al., 1997 and Wagner et al., 2001. The main difference is that expression in mammary tissue in the A line is more complete. Moreover, in the A line Cre expression also occurs in the female germline, which classifies the A line as a "germline deleter".
The WAP gene promoter targets gene expression exclusively to mammary epithelium. One line is available Wagner et al., 1997 and Wagner et al., 2001.
Gene knockout mice
The Stat5a gene was targeted Liu et al., 1997.
The entire locus containing the Stat5a and Stat5b genes was deleted. This was accomplished using Cre-loxP mediated recombination Cui et al., 2004. Stat5-null mice die perinataly.
Mice carrying "floxed" genes
Stat5a/b - Labs in Possession of these Mice
The entire locus containing the Stat5a and Stat5b genes was targeted with loxP sites Cui et al., 2004. The loxP sites bracket the locus. The 110 kbp locus can be deleted using various Cre expressing strains.
Bcl-x - Labs in Possession of these Mice
The Bcl-x gene was targeted with loxP sites Rucker et al., 2000. The Bcl-x gene can be deleted using a variety of Cre-expressing mice in a variety of tissues.
1. hematopoietic cells Wagner et al., 2000).
The NIDDK Office of Technology Transfer and Development is pleased to initiate access to a newly simplified and expedited process for providing research resources to scientists at academic and non-profit institutions. To request materials of NIDDK scientists, please visit our website at MTA. At this website, academic and non-profit Recipient Scientists and tech transfer professionals can access all the information and tools needed to provide us with a signed MTA. We are committed to processing the signed, unmodified agreements found on that page within about a day of our receipt. Upon our receipt of the properly signed, unmodified document, we will authorize transfer of the materials by the Provider Scientist. We will return a scanned, color Adobe pdf version of the fully executed document to you at the e-mail addresses you provide. We believe you will find the documents acceptable, however, SHOULD YOU REQUIRE ANY MODIFICATIONS TO THESE DOCUMENTS, DOWNLOAD THE MS WORD VERSION OF THE APPLICABLE DOCUMENT AND SEND A REDLINED, UNSIGNED VERSION REQUESTING THE MODIFICATIONS BY E-MAIL ATTACHMENT TO LINDA SCHILLING SO WE MAY CONSIDER YOUR REQUEST. We are pleased to be able to share research resources and seek to make these transfers with minimal administrative impediments so as to avoid delaying the moment when a research tool may be put to use in your laboratory.
For additional information contact Lothar Hennighausen