Instruments and Software used are:
GeneChip Hybridization Oven 640
GeneChip Hybridization Oven 320
GeneChip Fluidics Station 450
GeneChip Scanner 3000 7G with AutoLoader- can hold 48 arrays at a time, enabled for High-Resolution Scanning.
GeneChip Operating Software (GCOS) v1.1.1
Technical Documentation: http://www.affymetrix.com/support/technical/index.affx
Manuals : http://www.affymetrix.com/support/technical/manuals.affx
Technical Notes: http://www.affymetrix.com/support/technical/technotesmain.affx
Data Analysis: https://www.affymetrix.com/support/downloads/manuals/data_analysis_fundamentals_manual.pdf (log-in required)
NetAffx: http://www.affymetrix.com/analysis/index.affx (log-in required)
The concentration of the samples are deduced using the Nanodrop, and the RNA integrity is shown using the Bioanalyzer. The samples are then processed through varies steps including IVT labeling, Hybridization and Scanning. The raw data (Image files) is analyzed and packaged by our bioinformatics group.