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Sponsored by: |
National Cancer Institute (NCI) |
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Information provided by: | National Institutes of Health Clinical Center (CC) |
ClinicalTrials.gov Identifier: | NCT00341991 |
This case-control study was planned to investigate the link of solar radiation with gene damage, host factors, and DNA repair proficiency in cutaneous malignant melanoma (CMM) risk. The hypothesis was that impaired DNA repair proficiency is associated with an increased risk of CMM due to unrepaired DNA damage, particularly in subjects with dysplastic nevi, poor tanning ability or genetic susceptibility.
The study was reviewed as an RO1 Grant from the National Cancer Institute in 1995. Subject enrollment, which included clinical evaluation, epidemiologic questionnaires, and skin and blood sample collection, was completed in 1999 on approximately 180 melanoma cases and 180 controls identified in Italy. The study protocol and consent form both included the measurement of genetic and biochemical factors and DNA repair capacity. DNA repair proficiency was measured in lymphocytes by the host cell reactivation assay, and sun exposure was evaluated by means of a detailed questionaire. Photographs of the back of the subjects were taken to allow nevi count. Minimal erythemal dosage was measured in all subjects to estimate skin sun sensitivity 24 hours after skin's UV-irradiation. Skin color was ascertained on the inner side of the forearm by means of a Minolta chromometer.
The aim of this protocol is to continue analysis of the biological samples already collected, as originally outlined in the study protocol. In particular, we plan to measure polymorphisms in genes that may lead to susceptibility to melanoma. Initially we will concentrate on variation in genes involved in repairing damaged DNA, but plan to look at a broad group of candidate susceptibility genes.
Condition |
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Melanoma |
Study Type: | Observational |
Official Title: | Molecular Epidemiology of Cutaneous Malignant Melanoma |
Estimated Enrollment: | 1600 |
Study Start Date: | October 2001 |
This case-control study was planned to investigate the link of solar radiation with gene damage, host factors, including also genetic variants, and DNA repair proficiency in cutaneous malignant melanoma (CMM) risk. The hypothesis was that impaired DNA repair proficiency is associated with an increased risk of CMM due to unrepaired DNA damage, particularly in subjects with dysplastic nevi, poor tanning ability or genetic susceptibility.
The study was reviewed and funded as an R01 Grant from the National Cancer Institute in 1995. Subject enrollment, which included clinical evaluation, epidemiologic questionnaires, and skin and blood sample collection, was completed in 1999 on approximately 180 melanoma cases and 180 controls identified in Italy. The study protocol and consent form both included the measurement of genetic and biochemical factors, and DNA repair capacity. DNA repair proficiency was measured in lymphocytes by the host cell reactivation assay, and sun exposure was evaluated by means of a detailed questionnaire. Photographs of the back of the subjects were taken to allow nevi count. Minimal erythemal dosage was measured in all subjects to estimate skin sun sensitivity 24 hours after skin's UV-irradiation. Skin color was ascertained on the inner side of the forearm by means of a Minolta chromometer.
The aim of this protocol is to continue analysis of the biological samples already collected, as originally outlined in the study protocol. In particular, we measured polymorphisms in genes that may lead to susceptibility to melanoma by altering the repair of damaged DNA. In addition, we investigated the role of pigmentation genes, including MC1R, ASIP and AGRP in melanogenesis, and their interaction with sun exposure and DNA repair. We have found that variants in the MCIR gene are associated with risk of developing melanoma, and in particular, the type of melanomas that arise on skin with no marked signs of chronic solar damage and that bears mutations in the BRAF oncogene. We are currently analyzing other genes in the pigmentation pathway. We have also completed the laboratory analyses of the protein pattern in plasma of all subjects and we are conducting the related statistical analyses, to investigate whether they differ by case-status, presence of dysplastic nevi, gene polymorphisms or DNA repair proficiency. Moreover, we are studying the effect of variants in KIR genes and protection against melanoma risk.
Ages Eligible for Study: | 20 Years to 75 Years |
Genders Eligible for Study: | Both |
Accepts Healthy Volunteers: | No |
Study ID Numbers: | 999902035, 02-C-N035 |
Study First Received: | June 19, 2006 |
Last Updated: | September 23, 2008 |
ClinicalTrials.gov Identifier: | NCT00341991 |
Health Authority: | United States: Federal Government |
DNA Repair Genes Pigmentation Skin Cancer Sun Exposure |
Neuroectodermal Tumors Skin Diseases Nevus, Pigmented Neoplasms, Germ Cell and Embryonal Melanoma, familial |
Neuroepithelioma Nevus Skin Neoplasms Neuroendocrine Tumors Melanoma |
Neoplasms Neoplasms by Site Neoplasms by Histologic Type Neoplasms, Nerve Tissue Nevi and Melanomas |