Illumina Sequencing Technology : NIDDK

Scientific Programs

Illumina Sequencing Technology

This technology can sequence millions of fragments using reversible terminator-based sequencing chemistry in a single run (more about this). Samples are provided from researchers for genomic DNA sequencing, small RNA discovery, protein-DNA interaction (ChIPSeq).


Flowcell has 8 lanes which enables the simultaneous processing of eight samples for single-end sequencing. We reserve one lane for internal control.

The Genomic Core Facility generates clonal clusters from single molecule fragments on the flowcells at the cluster station. Different primers can be used in a single flowcell.

Cluster Station

The clusters are sequenced on the Genome Analyzer
. This system can generate highly accurate results, providing more than 3.5 million tags.

Genome Analyzer

Our Bioinformatics team can process the image, and generate the base calling and alignment.
Short sequence reads are aligned against a reference genome and genetic differences are called using specially developed data analysis pipeline software. The Genomic Core Facility offers summary and preliminary statistics along with the delivery of sequencing data.



Page last updated: January 01, 0001

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