| | Other Issues: previous | next | latest | archiveResearch Articles Quantification of the CBD-FITC conjugates surface coating on cellulose fibres Ricardo Pinto, António L Amaral, Eugénio C Ferreira, Manuel Mota, Manuel Vilanova, Katia Ruel, and Miguel Gama PMCID: PMC2254392 Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations Angelika Wegerer, Tianqi Sun, and Josef Altenbuchner PMCID: PMC2254391 Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment Natalie Gavrilov-Yusim, Ekaterina Hahiashvili, Marina Tashker, Victoria Yavelsky, Ohad Karnieli, and Leslie Lobel PMCID: PMC2254390 Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface Zhengbing Jiang, Bei Gao, Ren Ren, Xingyi Tao, Yushu Ma, and Dongzhi Wei PMCID: PMC2267459 Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells Khajornsak Tragoolpua, Nutjeera Intasai, Watchara Kasinrerk, Sabine Mai, Yuan Yuan, and Chatchai Tayapiwatana PMCID: PMC2258298 Laser surgery of zebrafish (Danio rerio) embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the laser's effect on short- and long-term development Vikram Kohli and Abdulhakem Y Elezzabi PMCID: PMC2270268 Spherezymes: A novel structured self-immobilisation enzyme technology Dean Brady, Justin Jordaan, Clinton Simpson, Avashnee Chetty, Cherise Arumugam, and Francis S Moolman PMCID: PMC2266724 Recombinant gas vesicles from Halobacterium sp. displaying SIV peptides demonstrate biotechnology potential as a pathogen peptide delivery vehicle Marinko Sremac and Elizabeth S Stuart PMCID: PMC2270826 Improved RNA quality and TaqMan® Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials Jinghuan Li, Paul Smyth, Susanne Cahill, Karen Denning, Richard Flavin, Sinead Aherne, Marco Pirotta, Simone M Guenther, John J O'Leary, and Orla Sheils PMCID: PMC2259333 Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection Gudrun Schiedner, Sabine Hertel, Corinna Bialek, Helmut Kewes, Gero Waschütza, and Christoph Volpers PMCID: PMC2262890 Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris Mario Canales, José M Pérez de la Lastra, Victoria Naranjo, Ard M Nijhof, Michelle Hope, Frans Jongejan, and José de la Fuente PMCID: PMC2262073 Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coli protein, RpoS Jin-Seung Park, Kyung-Yeon Han, Jong-Ho Lee, Jong-Am Song, Keum-Young Ahn, Hyuk-Seong Seo, Sang-Jun Jun Sim, Seung-Wook Kim, and Jeewon Lee PMCID: PMC2278137 Simplified approaches for the development of an ELISA to detect circulating autoantibodies to p53 in cancer patients Ratchada Cressey, Saranya Pimpa, Busyamas Chewaskulyong, Nirush Lertprasertsuke, Somchareon Saeteng, Chatchai Tayapiwatana, and Watchara Kasinrerk PMCID: PMC2275332 Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET Catarina Madeira, Luís MS Loura, Manuel Prieto, Aleksander Fedorov, and M Raquel Aires-Barros PMCID: PMC2275333 Systolic and diastolic ventricular function in zebrafish embryos: Influence of norepenephrine, MS-222 and temperature Martin A Denvir, Carl S Tucker, and John J Mullins PMCID: PMC2291041 Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers Charlie Yu Ming Hsu and Hasan Uludağ PMCID: PMC2275331 Intracellular delivery of peptides via association with ubiquitin or SUMO-1 coupled to protein transduction domains Anne-Laure Vitte and Pierre Jalinot PMCID: PMC2287181 Glycosyltransferase efficiently controls phenylpropanoid pathway Anna Aksamit-Stachurska, Alina Korobczak-Sosna, Anna Kulma, and Jan Szopa PMCID: PMC2294120 Design of a zinc finger protein binding a sequence upstream of the A20 gene Yong Wei, Dajun Ying, Chunli Hou, Xiaoping Cui, and Chuhong Zhu PMCID: PMC2278136 Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorpha Loknath Gidijala, Roel AL Bovenberg, Paul Klaassen, Ida J van der Klei, Marten Veenhuis, and Jan AKW Kiel PMCID: PMC2287180 Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection Marine Goffinet, Patrick Chinestra, Isabelle Lajoie-Mazenc, Claire Medale-Giamarchi, Gilles Favre, and Jean-Charles Faye PMCID: PMC2323369 Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice Jason H Williams, Rebecca C Schray, Shashank R Sirsi, and Gordon J Lutz PMCID: PMC2362111 Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins Yoon-Sik Kim, Yong-Hwa Lee, Hyun-Soon Kim, Mi-Sun Kim, Kyu-Woong Hahn, Jeong-Heon Ko, Hyouk Joung, and Jae-Heung Jeon PMCID: PMC2335101 Killing cancer cells by targeted drug-carrying phage nanomedicines Hagit Bar, Iftach Yacoby, and Itai Benhar PMCID: PMC2323368 Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections Silvia Panseri, Carla Cunha, Joseph Lowery, Ubaldo Del Carro, Francesca Taraballi, Stefano Amadio, Angelo Vescovi, and Fabrizio Gelain PMCID: PMC2358889 In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system Andrea Predonzani, Francesca Arnoldi, Alejandro López-Requena, and Oscar R Burrone PMCID: PMC2373293 Differential protein expression following low temperature culture of suspension CHO-K1 cells Niraj Kumar, Patrick Gammell, Paula Meleady, Michael Henry, and Martin Clynes PMCID: PMC2386802 Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1 Qing Song and Xiaobo Zhang PMCID: PMC2390534 Hydroxycamptothecin-loaded nanoparticles enhance target drug delivery and anticancer effect Anxun Wang and Su Li PMCID: PMC2386803 Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges Thomas Naiser, Oliver Ehler, Jona Kayser, Timo Mai, Wolfgang Michel, and Albrecht Ott PMCID: PMC2435543 Viral vectors based on bidirectional cell-specific mammalian promoters and transcriptional amplification strategy for use in vitro and in vivo Beihui Liu, Julian F Paton, and Sergey Kasparov PMCID: PMC2396617 Substantially improved pharmacokinetics of recombinant human butyrylcholinesterase by fusion to human serum albumin Yue-Jin Huang, Paul M Lundy, Anthoula Lazaris, Yue Huang, Hernan Baldassarre, Bin Wang, Carl Turcotte, Mélanie Côté, Annie Bellemare, Annie S Bilodeau, Sandra Brouillard, Madjid Touati, Peter Herskovits, Isabelle Bégin, Nathalie Neveu, Eric Brochu, Janice Pierson, Duncan K Hockley, Douglas M Cerasoli, David E Lenz, Harvey Wilgus, Costas N Karatzas, and Solomon Langermann PMCID: PMC2415180 Expression of HIV-1 antigens in plants as potential subunit vaccines Ann Meyers, Ereck Chakauya, Enid Shephard, Fiona L Tanzer, James Maclean, Alisson Lynch, Anna-Lise Williamson, and Edward P Rybicki PMCID: PMC2443125 Three-dimensional culture of human meniscal cells: Extracellular matrix and proteoglycan production Helen E Gruber, David Mauerhan, Yin Chow, Jane A Ingram, H James Norton, Edward N Hanley, Jr, and Yubo Sun PMCID: PMC2443126 Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells Monika Witusik, Sylwester Piaskowski, Krystyna Hulas-Bigoszewska, Magdalena Zakrzewska, Sylwia M Gresner, S Ausim Azizi, Barbara Krynska, Pawel P Liberski, and Piotr Rieske PMCID: PMC2488339 Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR Ian Roberts, Grace Ng, Nicola Foster, Margaret Stanley, Michael T Herdman, Mark R Pett, Andrew Teschendorff, and Nicholas Coleman PMCID: PMC2529285 A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based Jason R Hillebrecht and Shaorong Chong PMCID: PMC2507708 Genomic insertion of lentiviral DNA circles directed by the yeast Flp recombinase Brian Moldt, Nicklas H Staunstrup, Maria Jakobsen, Rafael J Yáñez-Muñoz, and Jacob G Mikkelsen PMCID: PMC2538528 Plasmid selection in Escherichia coli using an endogenous essential gene marker Shan Goh and Liam Good PMCID: PMC2527308 Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells Martin Loignon, Sylvie Perret, John Kelly, Denise Boulais, Brian Cass, Louis Bisson, Fatemeh Afkhamizarreh, and Yves Durocher PMCID: PMC2538527 Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV) Martina Inga Kirsch, Birgit Hülseweh, Christoph Nacke, Torsten Rülker, Thomas Schirrmann, Hans-Jürgen Marschall, Michael Hust, and Stefan Dübel PMCID: PMC2543005 Phosphoribosyl pyrophosphate synthetase activity affects growth and riboflavin production in Ashbya gossypii Alberto Jiménez, María A Santos, and José L Revuelta PMCID: PMC2551608 Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from Yeast Alessandra Mallano, Silvia Zamboni, Giulia Carpinelli, Filippo Santoro, Michela Flego, Alessandro Ascione, Mara Gellini, Marina Tombesi, Franca Podo, and Maurizio Cianfriglia PMCID: PMC2547108 Measuring microRNAs: Comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods Robert A Ach, Hui Wang, and Bo Curry PMCID: PMC2547107 Allogeneic and autogenous transplantations of MSCs in treatment of the physeal bone bridge in rabbits Ladislav Planka, Petr Gal, Helga Kecova, Jiri Klima, Jana Hlucilova, Eva Filova, Evzen Amler, Petr Krupa, Leos Kren, Robert Srnec, Lucie Urbanova, Jana Lorenzova, and Alois Necas PMCID: PMC2556323 The stabilizing effects of immobilization in D-amino acid oxidase from Trigonopsis variabilis Iskandar Dib and Bernd Nidetzky PMCID: PMC2557008 Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii Kateryna Sybirna, Tatiana Antoine, Pia Lindberg, Vincent Fourmond, Marc Rousset, Vincent Méjean, and Hervé Bottin PMCID: PMC2553784 Pharmaceutical induction of ApoE secretion by multipotent mesenchymal stromal cells (MSCs) Suzanne Zeitouni, Brian S Ford, Sean M Harris, Mandolin J Whitney, Carl A Gregory, and Darwin J Prockop PMCID: PMC2596794 Development of a strategy to functionalize a dextrin-based hydrogel for animal cell cultures using a starch-binding module fused to RGD sequence Susana M Moreira, Fábia K Andrade, Lucíla Domingues, and Miguel Gama PMCID: PMC2577101 Pseudomonas fluorescens biofilms subjected to phage phiIBB-PF7A Sanna Sillankorva, Peter Neubauer, and Joana Azeredo PMCID: PMC2584026 Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescens Sanna Sillankorva, Peter Neubauer, and Joana Azeredo PMCID: PMC2582237 Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acid Dae-Kyun Ro, Mario Ouellet, Eric M Paradise, Helcio Burd, Diana Eng, Chris J Paddon, Jack D Newman, and Jay D Keasling PMCID: PMC2588579 Differential solubility of curcuminoids in serum and albumin solutions: implications for analytical and therapeutic applications Wolfgang W Quitschke PMCID: PMC2612664 A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65) Xiaofeng Wang, Martin Brandsma, Reynald Tremblay, Denis Maxwell, Anthony M Jevnikar, Norm Huner, and Shengwu Ma PMCID: PMC2621204 Preparation of alginate coated chitosan microparticles for vaccine delivery XingYi Li, XiangYe Kong, Shuai Shi, XiuLing Zheng, Gang Guo, YuQuan Wei, and ZhiYong Qian PMCID: PMC2603011 Large-scale preparation of active caspase-3 in E. coli by designing its thrombin-activatable precursors Hyo Jin Kang, Young-mi Lee, Yu-Jin Jeong, Kyoungsook Park, Mi Jang, Sung Goo Park, Kwang-Hee Bae, Moonil Kim, and Sang J Chung PMCID: PMC2621203 Methodology Articles Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail Salim Essakali, Dennis Carney, David Westerman, Peter Gambell, John F Seymour, and Alexander Dobrovic PMCID: PMC2254389 Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter Feng Fang, Giovanni Antico, Jiamao Zheng, and Charles V Clevenger PMCID: PMC2268924 High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis Hsueh-Wei Chang, Chun-An Cheng, De-Leung Gu, Chia-Che Chang, San-Hua Su, Cheng-Hao Wen, Yii-Cheng Chou, Ta-Ching Chou, Cheng-Te Yao, Chi-Li Tsai, and Chien-Chung Cheng PMCID: PMC2259332 Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues Dan Frumkin, Adam Wasserstrom, Shalev Itzkovitz, Alon Harmelin, Gideon Rechavi, and Ehud Shapiro PMCID: PMC2266725 Quantification of Cre-mediated recombination by a novel strategy reveals a stable extra-chromosomal deletion-circle in mice Wouter N Leonhard, Jeroen H Roelfsema, Irma S Lantinga-van Leeuwen, Martijn H Breuning, and Dorien JM Peters PMCID: PMC2277394 Enhanced gene expression from retroviral vectors Magnus Blø, David R Micklem, and James B Lorens PMCID: PMC2266726 Protein kinase substrate identification on functional protein arrays Lihao Meng, Gregory A Michaud, Janie S Merkel, Fang Zhou, Jing Huang, Dawn R Mattoon, and Barry Schweitzer PMCID: PMC2270825 Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms Meti Buh Gašparič, Katarina Cankar, Jana Žel, and Kristina Gruden PMCID: PMC2322970 Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography Eva Sewekow, Lars Christian Keßler, Andreas Seidel-Morgenstern, and Hermann-Josef Rothkötter PMCID: PMC2292165 RNAi-mediated gene silencing in tick synganglia: A proof of concept study Shahid Karim, Bronwyn Kenny, Emily Troiano, and Thomas N Mather PMCID: PMC2386130 Gene targeting in adult rhesus macaque fibroblasts Daniel T Meehan, Mary Ann Zink, Melissa Mahlen, Marilu Nelson, Warren G Sanger, Shoukhrat M Mitalipov, Don P Wolf, Michel M Ouellette, and Robert B Norgren, Jr PMCID: PMC2292692 A set of ligation-independent in vitro translation vectors for eukaryotic protein production Viola Bardóczy, Viktória Géczi, Tatsuya Sawasaki, Yaeta Endo, and Tamás Mészáros PMCID: PMC2311287 Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system Maria Ruottinen, Monika Bollok, Martin Kögler, Antje Neubauer, Mirja Krause, Eija-Riitta Hämäläinen, Johanna Myllyharju, Antti Vasala, and Peter Neubauer PMCID: PMC2315644 Comparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices, vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method Ta-Chin Lin, Jui-Mei Yen, Tsung-Cheng Kuo, Kun-Bing Gong, Kung-Hao Hsu, and Teng-Tsao Hsu PMCID: PMC2364622 Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases Takako Noguchi, Masaaki Ikeda, Yoshihiro Ohmiya, and Yoshihiro Nakajima PMCID: PMC2375886 PCR-based gene synthesis to produce recombinant proteins for crystallization Damien Marsic, Ronny C Hughes, Miranda L Byrne-Steele, and Joseph D Ng PMCID: PMC2408586 Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production Josef Schulze-Horsel, Yvonne Genzel, and Udo Reichl PMCID: PMC2408585 A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR Robert G Rutledge and Don Stewart PMCID: PMC2397388 Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis Huajun Qin, Jian Hu, Yuanzhi Hua, Shridhar V Challa, Timothy A Cross, and Fei P Gao PMCID: PMC2396618 Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cells Leonardo D'Aiuto, Clinton S Robison, Margherita Gigante, Edward Nwanegbo, Benjamin Shaffer, Meena Sukhwani, Carlos A Castro, and J Richard Chaillet PMCID: PMC2442052 Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV Michael C Wehr, Lisa Reinecke, Anna Botvinnik, and Moritz J Rossner PMCID: PMC2483975 Detection of N-acyl homoserine lactones using a traI-luxCDABE-based biosensor as a high-throughput screening tool Steve P Bernier, Anne L Beeston, and Pamela A Sokol PMCID: PMC2518144 Comparison of the mismatch-specific endonuclease method and denaturing high-performance liquid chromatography for the identification of HBB gene mutations Chia-Cheng Hung, Yi-Ning Su, Chia-Yun Lin, Yin-Fei Chang, Chien-Hui Chang, Wen-Fang Cheng, Chi-An Chen, Chien-Nan Lee, and Win-Li Lin PMCID: PMC2525636 Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure Natalia I Minaeva, Evgeny R Gak, Danila V Zimenkov, Aleksandra Yu Skorokhodova, Irina V Biryukova, and Sergey V Mashko PMCID: PMC2532685 Display of a Maize cDNA library on baculovirus infected insect cells Helene Y Meller Harel, Veronique Fontaine, Hongying Chen, Ian M Jones, and Paul A Millner PMCID: PMC2527309 Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression Carole Bougault, Anne Paumier, Elisabeth Aubert-Foucher, and Frédéric Mallein-Gerin PMCID: PMC2556324 Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin Xiu-Mei Cai, Hai-Long Xie, Ming-Zhu Liu, and Xi-Liang Zha PMCID: PMC2562374 One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester Maartje MC Bastings, Ingrid van Baal, EW Meijer, and Maarten Merkx PMCID: PMC2570673 A construct with fluorescent indicators for conditional expression of miRNA Linghua Qiu, Hongyan Wang, Xugang Xia, Hongxia Zhou, and Zuoshang Xu PMCID: PMC2569932 Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos Francisca Alcaraz-Pérez, Victoriano Mulero, and María L Cayuela PMCID: PMC2596795 Recombinant amyloid beta-peptide production by coexpression with an affibody ligand Bertil Macao, Wolfgang Hoyer, Anders Sandberg, Ann-Christin Brorsson, Christopher M Dobson, and Torleif Härd PMCID: PMC2606684 Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions Mubasher Dar, Theresa Giesler, Rob Richardson, Christine Cai, Mike Cooper, Shahin Lavasani, Peter Kille, Thierry Voet, and Joris Vermeesch PMCID: PMC2613886 Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems Kevin Rozwadowski, Wen Yang, and Sateesh Kagale PMCID: PMC2601046 Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology Britta Hasemeier, Matthias Christgen, Hans Kreipe, and Ulrich Lehmann PMCID: PMC2605753 Simultaneous tracking of fly movement and gene expression using GFP Dhruv Grover, Junsheng Yang, Simon Tavaré, and John Tower PMCID: PMC2625341 |
